Lactobacillus lactis CKDB001 ameliorate progression of nonalcoholic fatty liver disease through of gut microbiome: addendum

ABSTRACT

According to our recent study (N.Y. LEE et al. Gut Microbes 2020; 11:882–99.)¹, we reported that Lactobacillus and Pediococcus ameliorate progression of nonalcoholic fatty liver disease through modulation of the gut microbiome. According on the analysis method (Previous: 16s rRNA sequencing and Recent: whole gene sequencing), the probiotics named Lactobacillus bulgaricus that we used in the experiment was identified as Lactobacillus delbrueckii subsp. bulgaricus through 16s rRNA sequencing analysis. Recently, we performed a clearer analysis with whole gene sequencing to proceed with the clinical trial, it was identified as Lactobacillus delbrueckii subsp. lactis by whole gene sequencing. Therefore, we inform that the subspecies have been changed to lactis through WGS. Read L. bulgaricus in the previous paper as L. lactis. In this addendum, the results of the change to L. lactis are summarized, and descriptions have been added to Materials & methods and Discussion.

KEYWORDS:

• Word
• Lactobacillus delbrueckii subsp. lactis
• nonalcoholic fatty liver disease
• 16s rRNA sequencing
• Whole gene sequencing

Author contributions

NYL and KTS designed the study, interpreted the work, and wrote the manuscript. All authors participated in the experiments.

Disclosure of potential conflicts of interest

Author BYK were employed by company ChunLab, Inc. Authors BKJ and HCJ were employed by company CKDBiO. All other authors attest that there are no commercial associations that might be a conflict of interest in relation to the submitted manuscript.

Ethical approval statement

The animals received humane care and all procedures were performed in accordance with National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. All procedures were approved by the Institutional Animal Care and Use Committee of the College of Medicine, Hallym University (2018-04).

Additional information

Funding

This research was supported by Hallym University Research Fund, Korea National Research Foundation (NRF-2018M3A9F3020956, NRF-2019R1I1A3A01060447, and NRF-2020R1A6A1A03043026), and Hallym University Research Fund 2018 (HURF-2018-67).