Antibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death

Abstract

P-selectin glycoprotein ligand-1 (PSGL-1) has been proved to serve as the functional receptor for enterovirus 71 (EV71). We found the abundant expression of PSGL-1 on monocyte-derived dendritic cells (MDDCs). However, we have previously demonstrated that MDDCs did not support efficient replication of EV71. Dendritic cells (DCs) have been described to be subverted by various viruses including EV71 for viral dissemination, we thus explore the potential contribution of PSGL-1 on DC-mediated EV71 transmission. We found that the cell-surface-expressing PSGL-1 on MDDCs mediated EV71 binding, and intriguingly, these loaded-viruses on MDDCs could be transferred to encountered target cells; Prior-treatment with PSGL-1 antibodies or interference with PSGL-1 expression diminished MDDC-mediated EV71 transfer and rescued virus-induced cell death. Our data uncover a novel role of PSGL-1 in DC-mediated EV71 spread, and provide an insight into blocking primary EV71 infection.

Keywords:

• Dendritic cell
• enterovirus 71
• viral transmission
• P-selectin glycoprotein ligand-1

Disclosure of Potential Conflicts of Interest

No potential conflicts of interest were disclosed.

Funding

H.-B. W thanks the grant supports from Youth Innovation Promotion Association, the Chinese Academy of Sciences, and Natural Science Foundation of Shanghai (No.13ZR1445400). This work was also supported by grant received by J.-H. W from the Interdisciplinary and Collaboration Team of the Chinese Academy of Sciences.