![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
ABSTRACT
Bacterial lipooligosaccharide (LOS) is an important virulence-associated factor, and its sialylation largely confers its ability to mediate cell adhesion, invasion, inflammation, and immune evasion. Here, we investigated the function of the Haemophilus parasuis α-2,3-sialyltransferase gene, lsgB, which determines the terminal sialylation of LOS, by generating a lsgB deletion mutant as well as a complementation strain. Our data indicate a direct effect of lsgB on LOS sialylation and reveal important roles of lsgB in promoting the pathogenicity of H. parasuis, including adhesion to and invasion of porcine cells in vitro, bacterial load and survival in vivo, as well as a contribution to serum resistance. These observations highlight the function of lsgB in mediating LOS sialylation and more importantly its role in H. parasuis infection. These findings provide a more profound understanding of the pathogenic mechanism of this disease-causing bacterium.
Abbreviations
| LOS | = | lipooligosaccharide |
| NAD | = | nicotinamide adenine dinucleotide |
| CMP | = | Cytidine monophosphate |
| MP-Neu5Ac | = | Cytidine monophosphate-N-Acetylneuraminic acid |
| TSA | = | tryptic soy agar |
| TSB | = | tryptic soy broth |
| PK15 | = | porcine kidney cells |
| PIEC | = | porcine iliac artery endothelial cells |
| OD600 | = | optical density at 600 nm |
| CFUs | = | colony forming units |
| HPLC | = | high performance liquid chromatography |
| SEM | = | scanning electron microscopy |
| DMB | = | 1,2-diamino-4,5-methyleneoxybenzene |
| NS | = | normal serum |
| HS | = | heat-treated serum |
| fH | = | factor H |
| HBSS | = | Hanks’ Balanced Salt Solution |
| MAC | = | membrane attack complex |
| GalR | = | galactose binding lectin domain |
| Kan | = | kanamycin |
| Gm | = | gentamicin |
Acknowledgments
We thank Jeremy Allen, PhD, from Liwen Bianji, Edanz Group China for editing and improving the language.
Disclosure statement
No potential conflict of interest was reported by the authors.