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Research Paper

Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19

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Pages 890-902 | Received 26 Jan 2022, Accepted 28 Apr 2022, Published online: 19 May 2022
 

ABSTRACT

Antibodies to SARS-CoV-2 are central to recovery and immunity from COVID-19. However, the relationship between disease severity and the repertoire of antibodies against specific SARS-CoV-2 epitopes an individual develops following exposure remains incompletely understood. Here, we studied seroprevalence of antibodies to specific SARS-CoV-2 and other betacoronavirus antigens in a well-annotated, community sample of convalescent and never-infected individuals obtained in August 2020. One hundred and twenty-four participants were classified into five groups: previously exposed but without evidence of infection, having no known exposure or evidence of infection, seroconverted without symptoms, previously diagnosed with symptomatic COVID-19, and recovered after hospitalization with COVID-19. Prevalence of IgGs specific to the following antigens was compared between the five groups: recombinant SARS-CoV-2 and betacoronavirus spike and nucleocapsid protein domains, peptides from a tiled array of 22-mers corresponding to the entire spike and nucleocapsid proteins, and peptides corresponding to predicted immunogenic regions from other proteins of SARS-CoV-2. Antibody abundance generally correlated positively with severity of prior illness. A number of specific immunogenic peptides and some that may be associated with milder illness or protection from symptomatic infection were identified. No convincing association was observed between antibodies to Receptor Binding Domain(s) (RBDs) of less pathogenic betacoronaviruses HKU1 or OC43 and COVID-19 severity. However, apparent cross-reaction with SARS-CoV RBD was evident and some predominantly asymptomatic individuals had antibodies to both MERS-CoV and SARS-CoV RBDs. Findings from this pilot study may inform development of diagnostics, vaccines, and therapeutic antibodies, and provide insight into viral pathogenic mechanisms.

Acknowledgements

The authors gratefully acknowledge the study participants and the elected officials and employees of the City of Chelsea, including Mayor Tom Ambrosino. Grace Kirkpatrick, Diane Yang, Ju Cheng, Alex Kuo, Wendy Yang, and Maristela Onozato were instrumental in sample collection.

Disclosure statement

T.E.M. discloses financial interest in Telomere Diagnostics and Care Access Research, unrelated to this manuscript. S.M., M.D.S., R.K.W., and R.D.P. are employees of Cell Signaling Technology, Inc. The other authors report no conflict of interest.

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/21505594.2022.2073025.

Additional information

Funding

This work was supported by the VIC Innovation Fund (M.C.P.), CDC awards U01CK000490 and U01CK000633 (E.T.R., R.C.L., R.C.C.), and grants from the Lambertus Family Foundation (A.J.I.)