A novel aquaporin Aagp contributes to Streptococcus suis H₂O₂ efflux and virulence

ABSTRACT

Streptococcus suis is a bacterium that can cause infections in pigs and humans. Although oxidative stress is common occurrence during bacterial growth and infection, the regulation networks of S. suis under oxidative stress remain poorly understood. To address this, we utilized RNA-Seq to reveal the transcriptional landscape of S. suis in response to H₂O₂ stress. We identified novel genes responsible for S. suis resistance to oxidative stress, including those involved in DNA repair or protection, and essential for the biosynthesis of amino acids and nucleic acids. In addition, we found that a novel aquaporin, Aagp, belonging to atypical aquaglyceroporins and widely distributed in diverse S. suis serotypes, plays a crucial role during H₂O₂ stress. By performing oxidative stress assays and measuring the intracellular H₂O₂ concentrations of the wild-type strain and Aagp mutants during H₂O₂ stress, we found that Aagp facilitated H₂O₂ efflux. Additionally, we found that Aagp might be involved in glycerol transport, as shown by the growth inhibition and H₂O₂ production in the presence of glycerol. Mice infection experiments indicated that Aagp contributed to S. suis virulence. This study contributes to understanding the mechanism of S. suis oxidative stress response, S. suis pathogenesis, and the function of aquaporins in prokaryotes.

KEYWORDS:

• Streptococcus suis
• oxidative stress
• aquaporin
• virulence
• glycerol transport

Disclosure statement

No potential conflict of interest was reported by the authors.

Data Availability statement

The data supporting this study’s findings are available from the corresponding author upon reasonable request.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/21505594.2023.2249789

Additional information

Funding

This work was supported by the National Key Research and Development Program of China (No.2021YFD1800402), National Natural Science Foundation of China (No.32172859), Open Project Program of Jiangsu Key Laboratory of Zoonosis (No. R2103), Open Project Program of Engineering Research Center for the Prevention and Control of Animal Original Zoonosis, Fujian Province University (No.2021ZW001).