Retrieval of functional TCRs from single antigen-specific T cells: Toward individualized TCR-engineered therapies

Abstract

We have developed a highly versatile platform for the systematic retrieval of T-cell receptors (TCRs) from single-antigen-reactive T cells and for characterization of their function and specificity. This approach enables rapid extraction of multiple TCRs from repertoires in individuals and not only broadens the diversity of TCRs suitable for clinical use, but also sets the stage for actively personalized immunotherapeutic strategies.

Keywords:

• adoptive cell transfer
• epitope
• personalized immunotherapy
• T cell engineering
• T cell receptor

Disclosure of Potential Conflicts of Interest

T Omokoko is Head of TCR Discovery at BioNTech Cell & Gene Therapies GmbH and has ownership interest in a patent application. P Simon is Head of Immunoreceptor Validation at BioNTech Cell & Gene Therapies GmbH and has ownership interest in a patent application. O Tureci has ownership interest in a patent. U Sahin is a cofounder of BioNTech Cell & Gene Therapies GmbH, reports receiving a commercial research grant from BioNTech Cell & Gene Therapies GmbH, and has ownership interest (including patents) in TRON and BioNTech Cell & Gene Therapies GmbH.