Improved antibacterial function of Rifampicin-loaded solid lipid nanoparticles on Brucella abortus

Abstract

The objective of this study was to assess the antibacterial activity of Rifampicin-loaded solid lipid nanoparticles on Brucella abortus 544. Rifampicin-loaded solid lipid nanoparticles were prepared by a modified microemulsion/sonication method and characterized. The results showed the average size about 319.7 nm, PI about 0.20 and zeta potential about 18.4 mv, encapsulation efficacy and drug-loading were equal to 95.78 and 34.2%, respectively, with a spherical shape. Drug release lasted for 5 days. The antibacterial activity was statistically significant with p < .05 in bacterial and cell culture media compared to free Rifampicin. It can be concluded that solid lipid nanoparticles can be considered as a promising delivery system for improving the antibacterial activity of Rifampicin against Brucella abortus.

Keywords:

• Solid lipid nanoparticles
• rifampicin
• antibacterial activity
• Brucella abortus

Acknowledgements

We would like to thank, Andrea Schumacher for her kind support regarding providing material (Lipoid S-100, Lipoid Company, Germany), Dr. Mohammad Yousef Alikhani, Administrator of Microbiology Department and Brucellosis Centre for inter-institutional coordination. We also appreciate Dr. Abbas Farmani, Manager of Comprehensive Research Labs for preparing necessary types of equipment, and Ms. Samira Ataei, in charge of the Science and Technology Park for providing required equipment in making nanoparticles.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The findings described in this study are part of Ph.D. thesis that was funded by Vice-Chancellor for Research and Technology, Hamadan University of Medical Sciences [No. 9511126746].