A new method for early detection of pancreatic cancer biomarkers: detection of microRNAs by nanochannels

Abstract

Objective

To develop an effective new method for early detection of pancreatic cancer biomarkers and to aid early clinical diagnosis.

Methods

A DNA probe (Probe) capable of specifically recognizing the target miRNA was designed. The specific probe of miRNA 21 is designed first, and then mixed with the miRNA 21 sample to form a complex molecule, and the complex molecule is added to the nanochannels to detect the received signal. The probe is designed to detect the electrical signal by means of pre-matching and post-matching and observe the stability of the signal. The miRNA 21, miRNA 155, miRNA 196a were added to the nano-single channel to detect the characteristic signals and blocking time. The miRNA 21·probe 21 mixture was mixed with other five cancer-associated microRNAs, and the signal results of the detection were collected and compared.

Results

The signal of miRNA 21 was successfully detected. Whether the probe is designed at the front or the back, there are two signal results. The Probe should be designed to match the middle region of the miRNA. The three microRNA complex molecules have different characteristic signals and blocking times, which can be effectively distinguished.

Conclusion

Nanochannels can effectively detect pancreatic cancer-related microRNAs.

Keywords:

• MicroRNA
• biomarker
• pancreatic cancer
• nanochannels

Acknowledgements

Zhenfang Xiong and Zhonghua Fu are the co-corresponding authors of this article; Xiong Zhenfang is the first of the corresponding authors and Fu Zhonghua is the second of the corresponding authors.

Data availability

All data are available. Please contact us to access if it is needed.

Ethical approval and consent to participate

This article does not contain any studies with animals performed by any of the authors. All methods are carried out in accordance with relevant guidelines and regulations.

Author contribution statement

Jiasheng Xu: research design and drafting the manuscript.

Kialo Liao: literature search and helping to draft the manuscript.

Zhonghua Fu: help modify articles and collate references.

Zhenfang Xiong: review and revision of the manuscript and writing guidance.

Disclosure statement

No potential conflict of interest was reported by the authors.