Long non-coding RNA ROR mitigates cobalt chloride-induced hypoxia injury through regulation of miR-145

Abstract

Obstructive sleep apnoea-hypopnoea syndrome (OSAHS) is a condition causing apnea and hypopnea. LncRNA-ROR revealed properties in regulating hypoxia response. Our study explored the roles of ROR in CoCl₂-induced hypoxia injury in HK-2 cells. HK-2 cells were treated with CoCl₂ to induce hypoxia injury. Cell viability, cell apoptosis and apoptotic proteins were detected using CCK-8, flow cytometry and western blot, respectively. The alter expression of ROR and miR-145 was achieved through transfection. Moreover, the expressions of HIF-α and ERK and MAPK related factors were examined using western blot. We found that CoCl₂ decreased cell viability and increased apoptosis as well as increased the expression of ROR. ROR overexpression increased cell viability, decreased cell apoptosis. ROR overexpression upregulated anti-apoptotic proteins Bcl-2 and decreased p53, Bax and cleaved-Caspase-3. ROR overexpression also increased the expression of HIF-α. On the opposite, ROR silence led to the opposite results as relative to ROR overexpression. ROR overexpression decreased expression of miR-145. Co-transfection with ROR overexpression and miR-145 impaired the promoting effects of ROR in CoCl₂ treated cells. ROR increased phosphorylation of ERK while decreased phosphorylation of MAPK. In conclusion, lncRNA ROR alleviated CoCl₂-induced hypoxia injury through regulation of miR-145 as well as modulating ERK and MAPK signalling.

Highlights

1. CoCl₂ induces ROR upregulation;

2. Overexpression of ROR reduces CoCl₂-induced HK-2 cell injury;

3. Silence of ROR promotes CoCl₂-induced HK-2 cell injury;

4. Overexpression of ROR decreases miR-145 expression;

5. ROR overexpression modulates ERK and MAPK signalling pathways through regulation of miR-145.

Keywords:

• Obstructive sleep apnoea-hypopnoea syndrome
• LncRNA-ROR
• miR-145
• ERK
• MAPK

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Expression of Concern

Disclosure statement

No potential conflict of interest was reported by the authors.