Knockdown of lncRNA Gm11974 protect against cerebral ischemic reperfusion through miR-766-3p/NR3C2 axis

Abstract

Aims

In previous studies, numerous differential lncRNAs in cerebral ischemic reperfusion injury were identified using RNA-Seq analysis. However, little is known about whether and how lncRNAs involved in cerebral I/R injury. In this study, we investigated the function and explored the possible mechanism of lncRNA Gm11974 in cerebral I/R injury.

Methods

Oxygen glucose deprivation model in N2a cells were utilized to mimic the cerebral I/R injury in vitro. Trypan blue staining, Tunel, JC-1 and cell viability were measured to evaluate the function of lncRNA Gm11974. Dual-luciferase reporter assay was used to explore the potential mechanism of lncRNA Gm11974.

Results

Gm11974 was mainly located in cytoplasm. Knockdown of lncRNA Gm11974 alleviated the apoptosis induced by OGD and cell death rates were significantly reduced. We further provided the possible mechanism that Gm11974/miR-766-3p/NR3C2 axis plays important role in cerebral I/R injury.

Conclusions

We evaluated the function and mechanism of lncRNA Gm11974 in ischemic brain injury. LncRNA Gm11974 may serve as a potential target for new therapeutic intervention.

Keywords:

• ischemic stroke
• Gm11974
• Apoptosis
• miR-766-3p

Disclosure statement

All authors declare no conflicts of interest.

Additional information

Funding

The work is supported by Beijing Municipal Administration of Hospitals’Youth Program [grant number: QML20180506]; Natural Science Foundation of Beijing [grant number:17G10258]; Medical Elite Cultivation Program of Fujian, China [grant number:2014-ZQN-JC-38].