Sinomenine restrains breast cancer cells proliferation, migration and invasion via modulation of miR-29/PDCD-4 axis

Abstract

Sinomenine (Sino) is diffusely applied in heal rheumatoid arthritis and neuralgia. Howbeit, the activities of Sino in breast cancer cells remain confused. The research attempted to probe the anti-tumor function of Sino in breast cancer cells and divulge the feasible molecular mechanism. Sion at the 1–16 μM concentrations was exploited for the exposure of MDA-MB-231 or MCF7 cells, and cell growth, migration, invasion, cell cycle-relevant and apoptosis-correlative factors were estimated. Micro RNA (miR)-29 expression was evaluated via enforcing qRT-PCR, and the actions of miR-29 in MDA-MB-231 cells growth, migration and invasion were appraised after the overexpressed or suppressed vectors transfection. The functions of PDCD-4 in JNK and MEK/ERK pathways were estimated by employing western blot. We found that, Sino exposure impeded cell proliferation, provoked cell apoptosis and barricaded cell migration and invasion in MDA-MB-231 and MCF7 cells. Enhancement of miR-29 was observed in Sino-managed cells, and miR-29 overexpression further potentiated the activities of Sino in MDA-MB-231 cells. Additionally, Sino remarkably enhanced PCDC-4 expression via adjusting miR-29 in MDA-MB-231 cells. Beyond that, overexpressed PCDC-4 obstructed JNK and MEK/ERK pathways in MDA-MB-231 cells. Taken together, the explorations unveiled that Sino restrained MDA-MB-231 cells proliferation, migration, invasion, and provoked apoptosis through modulation of miR-29/PDCD-4 axis.

Highlight

1. Sino inhibits MDA-MB-231 and MCF7 cells proliferation and provokes apoptosis;

2. Sino restrains MDA-MB-231 and MCF7 cells migration and invasion;

3. Sino ascends miR-29 expression in MDA-MB-231 and MCF7 cells;

4. Sino adjusts cell growth, migration and invasion via modulating miR-29;

5. Sino up-regulates PDCD-4 expression through mediating miR-29;

6. PDCD-4 obstructs JNK and MEK/ERK pathways in MDA-MB-231 cells.

Keywords:

• Sinomenine
• microRNA-29
• breast cancer
• PDCD-4
• JNK
• MEK/ERK

View addendum:

Expression of Concern

Disclosure statement

Authors declare that there is no conflict of interests.

Author contributions

Conceived and designed the experiments: Guanglei Gao and Wenyan Ma; Performed the experiments and analyzed the data: Guanglei Gao, Xiaolin Liang and Wenyan Ma; Contributed reagents/materials/analysis tools: Xiaolin Liang; Wrote the manuscript: Guanglei Gao and Wenyan Ma.

Data availability statement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.