Isolation and Identification of Antibacterial Compounds from the Extracts of Garcinia indica and Curcuma aromatica, using Bioautography and Mass Spectrometric Techniques

Abstract

In the present study, Ethyl acetate extract of mature but not fully ripe fruit rinds of Garcinia indica and rhizomes of Curcuma aromatica were investigated for antibacterial activity against Xanthomonas campestris and Xanthomonas axonopodis pv. punicae by direct bioautography technique. The bioautography directly provides information about antimicrobial activity of substances separated from a mixture. The bioautography results revealed that, the Zone of inhibition for extract of fruit rinds of G. indica was observed at Rf 0.63 and for extract of rhizomes of C. aromatica zones of inhibition were observed at Rf 0.73 and 0.93. This indicates that the compounds retained at these Rf values have potent antibacterial activity. These compounds were isolated and identified using LC-MS/MS and GC-MS techniques.

Key words:

• Herbal extracts
• Antibacterial activity
• Isolation
• Bioautography
• LC-MS/MS
• GC-MS