![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
The fact that Gymnema sylvestre is often used in various herbal formulations has led us to develop an HPTLC densitometric method for the quantification of its bioactive constituents i.e. gymnemic acids. Gymnemic acids are a group of triterpenoids saponins with gymnemagenin as the common aglycone. For the development of the HPTLC based analysis method, the silica gel 60 F254 TLC plates (20 x 10 cm; 200 μm thickness) were used as the stationary phase and toluene-ethyl acetate-methanol-formic acid (60:20:15:05, v/v) as the mobile phase. The developed HPTLC densitometric method was validated as per ICH guidelines. The modified vanillin-sulphuric acid was used as visualization reagent and the plates were scanned at 500 nm using CAMAG TLC Scanner. The regression analysis of the calibration data showed an excellent linear relationship between peak area vs concentration of gymnemagenin. The linearity for gymnemagenin was found to be in the range of 500-3000 ng/band. The proposed method for the estimation of gymnemagenin was found to be linear (r2=0.9987), precise (RSD <1.5 % and <2 % for intra-day and inter-day precision, respectively), and accurate (mean recovery ranged between 98.43-101.44 %). The developed method wassuccessfully applied for quantification of gymnemic acids as gymnemagenin in extracts and an in-house herbal formulation containing G. sylvestre without any interference.