GRASP55 facilitates autophagosome maturation under glucose deprivation

ABSTRACT

Under growth condition, the Golgi reassembly-stacking protein of 55 kDa (GRASP55)/GORASP2 serves as the “glue” to hold adjacent Golgi cisternae into stacks by forming trans-oligomers. Upon glucose starvation, GRASP55 is de-O-GlcNAcylated and functions as a membrane tether to facilitate autophagosome-lysosome fusion through the interactions with LC3-II and LAMP2.

Keywords:

• GRASP55
• LC3
• LAMP2
• O-GlcNAcylation
• autophagosome-lysosome fusion

Disclosure of potential conflicts of interest

No potential conflicts of interest were disclosed.

Additional information

Funding

This work was supported in part by the National Institutes of Health (Grant GM112786), MCubed and the Fast Forward Protein Folding Disease Initiative of the University of Michigan to Y. Wang.