Complete mitochondrial genome of the non-biting midge Polypedilum henicurum Wang, 1995 (Diptera: Chironomidae)

Abstract

The complete mitochondrial genome of Polypedilum henicurum was sequenced and analyzed. The whole mitogenome is 15,964 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and two ribosomal RNAs (rRNAs). The overall nucleotide composition is 39.5% A, 39.0% T, 13.1% C, and 8.4% G. Most PCGs start with ATN codon excluding COX1 (TTG) and all PCGs end with TAA codon. Gene arrangement stays the same with the ancestral mitochondrial genome. Bayesian inference phylogenetic tree supports its close relationship with P. unifascium. This work enriches the library of Chironomidae mitochondrial genomes and provides a valuable resource for understanding the evolutionary history of Polypedilum.

Keywords:

• Chironomidae
• mitochondrial genome
• Polypedilum henicurum
• phylogeny

Author contributions

Xin Qi conceived the original idea and designed the work. Xuanru Di and Chao Song collected the specimens and carried out experiments. Teng Lei and Kunlun Mou analyzed experimental data. Teng Lei designed the figure and drafted the manuscript. All authors revised the final version of the manuscript and agreed to be accountable for all aspects of the work.

Disclosure statement

The authors report no conflicts of interest and are alone responsible for the content and writing of this paper.

Data availability statement

The complete mitochondrial genome of Polypedilum henicurum for this study is openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov with accession number MZ981735. The associated BioProject, BioSample, and SRA are deposited in the Genome Sequence Archive in National Genomics Data Center at https://ngdc.cncb.ac.cn under accession numbers PRJCA015227, SAMC1125797, and CRA010003.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China (NSFC) [Grant Nos. 32070481, 32100353], the Zhejiang Provincial Natural Science Foundation of China [Grant No. LY22C040003], the Science & Technology Project of Taizhou [Grant Nos. 21hb04, 21nya17], and the Training Program of Undergraduate Innovation in China [Grant Nos. 202210350031, 202110350023, 202110350060].