The complete mitochondrial genome of the Tanichthys flavianalis (Cypriniformes: Tanichthyidae) and its phylogeny

Abstract

In the present study, we have sequenced, annotated and characterized the complete mitogenome of Tanichthys flavianalis Li, Liao & Shen, 2022, which is an endemic minnow to Hainan Province, China. The entire mitogenome size is 16,545 bp, containing 37 genes coding for 13 mitochondrial proteins, 22 tRNAs, 2 rRNAs, and one putative control region. It has the typical fish mitochondrial gene arrangement, though with 9 gene overlaps and 17 intergenic spacers scattering throughout the mitogenome, and a high A + T bias (60.8%) comparing to other Cyprinids. ATG is used as the start codon in most mitochondrial protein coding genes (PCGs) except for cox1 which uses GTG, whereas the canonical TAA/TAG are prevailing stop codons except for cox2, cox3, nad4 and cytb. Phylogenetic analyses with other reported species from Tanichyidae were conducted using IQ-TREE and MrBayes, based on the concatenated 13 PCGs. The results clustered T. flavinalis with the newly described T. albiventris Li Bohlen & Liao, 2022, and proved the reciprocally monophyletic relationship between Chinese and Vietnamese Tanichthys.

Keywords:

• Hainan
• endemic minnow
• mitogenome
• long and accurate PCR
• protein coding genes

Acknowledgements

We thank four anonymous reviewers for their valuable comments on this article.

Ethical approval

Experiment permission was issued by the Ethics Committee of Xinyang Agriculture and Forestry University (XYNLXY20210911), and all procedures involving animal attendances were strictly followed the Chinese Association for the Laboratory Animal Sciences and the Institutional Animal Care and Use Committee (IACUC) protocols.

Author contributions

LZ and FL conceived and designed the study, and conducted fundraising. FL and CS collected the samples. TY performed the experiments and uploaded the sequence information to GenBank. YG analyzed data and wrote the manuscript. All authors have proofread the manuscript and agreed to publish this version.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The genome sequence data of the study are deposited in GenBank of NCBI (https://www.ncbi.nlm.nih.gov/) under the accession no. OM687296. The associated BioProject, Bio-Sample and Run numbers are PRJNA957284, SAMN34248125, and SRR25113585, respectively.

Additional information

Funding

The study was supported by the Opening project of CAS key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences [No. KXYSWS2205], the Research special project (basic research direction) of Shanghai Science & Technology Museum [No. SST M/SOP ZY-03-R2KZ-20220100012 and No. SST M/SOP ZY-03-R2KZ-20230100003], the Innovative Research Team of Ecological fisheries in Reservoirs of the Huai River Basin in Xinyang Agriculture and Forestry University [XNKJTD-016], and the Youth Fund Project of Xinyang Agriculture and Forestry College [No. QN2021020].