DNA barcodes and insights into the phylogenetic relationships of Corvidae (Aves: Passeriformes)

Abstract

DNA barcoding has become a promising tool for species identification and phylogeny in a wide range of animal taxa using mitochondrial cytochrome c oxidase subunit I (COI). The Corvidae (Aves: Passeriformes) is a species rich and morphologically diverse family. In the present study, we analyzed the COI barcodes of 39 species from 12 genera of Corvidae. COI gene was also used to examine phylogenetic relationships of Corvidae. Every species possessed a barcode distinct from that of other species. Kimura two-parameter distances were calculated between species barcodes. The average genetic distance between the species was 22 times higher compared to the average genetic distance within species. Maximum likelihood method was used to construct a phylogenetic tree. All the species could be discriminated by their distinct clades in the phylogenetic tree. COI gene data provided good evidence for the monophyly of the Corvidae. Members of Cyanopica and Pyrrhocorax were the first to split from the Corvidae lineage. Analysis of COI genes supported the others genera fell into two clades. DNA barcoding is an effective molecular tool for Corvidae species identification and phylogenetic inference.

Keywords:

• DNA barcode
• cytochrome c oxidase I
• Corvidae
• phylogenetic relationship

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31260088, 31560590), the Natural Science Foundation of Jiangxi Province (20152ACB21006) and the Science and Technology Foundation of Jiangxi Provincial Department of Education (GJJ150768).

Disclosure statement

The authors report no conflicts of interest.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [31260088, 31560590], the Natural Science Foundation of Jiangxi Province [20152ACB21006] and the Science and Technology Foundation of Jiangxi Provincial Department of Education [GJJ150768].