ABSTRACT
Oil nanocapsules (ONP) can increase oil solubility and also enhance the availability of their bioactive substances; however, little is known about their safety. This research provides an overview of the toxicity of ONP for food applications based on a search in the Scopus, Science Direct, and Pubmed/Medline databases. Among all the studies concerning ONP and food, only 5% have performed toxicity assessments; and 71.3%, 21.4%, and 7.3% involved essential, vegetable, and seaweed oils, respectively. All the studies evaluating cell viability were performed under in vitro conditions and most of them (85%) used MTT reduction assay. Only 1 study used in vivo experimental approaches based on a mouse model, although in vitro (MTT) analyses were also performed. About 43% of the studies reported decreases in the viability of healthy cells in the highest concentrations tested. Concerning the potential beneficial effects of ONP, there were 7 and 6 studies pointing to antioxidant and anti-cancer effects, respectively. Although there is no consensus on safety, there already are 97 patents using ONP in the food industry, indicating the need for further studies in this field to safely use this innovative technology.
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Acknowledgments
The authors are thankful to the National Council for Scientific and Technological Development (CNPq, Brazil) for the research funding (Process nº 423478/2016-8) and the Master’s scholarship (Process nº 132842/2019-0), and to the Coordination for the Training and Improvement of Higher Education Personnel (CAPES, Brazil).
Disclosure statement
No potential conflict of interest was reported by the author(s).
Notes
1. Adapted from Cephamls. Available: https://www.cephamls.com/srb-cytotoxicity-assay-kit-colorimetric/ Accessed: 07 Dec. 2021.
2. Adapted from: R&DSystems. Available: https://www.rndsystems.com/resources/protocols/flow-cytometry-protocol-analysis-cell-viability-using-propidium-iodide Accessed: 07 Dec. 2021.