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Original Articles

Monitoring Spoilage Bacteria and Wild Yeasts in Eastern Chinese Breweries

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Pages 43-47 | Published online: 01 Feb 2018
 

Abstract

Based on 100 isolated strains of beer-spoilage bacteria and 22 wild yeast strains, this article investigates the contamination degree assessment and quality control procedures of seven breweries in eastern China. Samples were collected from five main points in each brewery: brewing water and wort; fermented beer; seed yeasts; equipment, air, and surroundings; and finished beer. All of the isolates were identified to the genus or species level by comparing their 16s rDNA, and species of wild yeast were identified as non-Saccharomyces and Saccharomyces. Determination of microorganism growth in degassed commercial lager beer indicated that approx. 80% of the bacteria had the ability to contaminate beer. This study of the occurrence of contamination in eastern Chinese breweries indicates that monitoring spoilage microorganisms is important for enhancing the sanitation quality control procedures in these plants.

RESUMEN

Sobre la base de 100 cepas aisladas de las bacterias dañinas a la cerveza y 22 cepas de levaduras silvestres, este artículo se investiga la evaluación del grado de contaminación y los métodos de control de calidad de siete cervecerías del este de China. Las muestras fueron recogidas a partir de cinco puntos principales en cada cervecería: agua de cervecero y mosto; la cerveza fermentada; semillas de las levaduras; equipo, el aire, y el entorno; y la cerveza terminada. Todos los aislamientos fueron identificados a nivel de género o especie mediante la comparación de sus 16 rADN, y especies de levaduras silvestres fueron identificados como no-Saccharomyces y Saccharomyces. Determinación del crecimiento de los microorganismos en la cerveza lager comercial desgasificado indicó que aproximadamente 80% de las bacterias tiene la capacidad de contaminar la cerveza. Este estudio de la incidencia de contaminación en las cervecerías del este de China indica que la vigilancia de los microorganismos deterioros es importante para aumentar el saneamiento procedimientos de control de calidad en estas plantas.

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