Stepwise Elution of Hop Resin Constituents from QAE Sephadex A-25 Ion-Exchange Resin¹

1 Contribution of the Oregon Agricultural Experiment Station in cooperation with the Agricultural Research Service, U.S. Department of Agriculture, both at Corvallis, OR 97331; Technical Paper No. 4543 of the former. This work was supported in part by the U.S. Brewers Association.

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2 Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the above agencies, and does not imply its approval to the exclusion of other products that may also be suitable.

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3 Presented at the 43rd Annual Meeting, St. Louis, May 1977.

Abstract

A method was developed for the chromatographic separation of hop constituents by stepwise elution. A column of QAE Sephadex A-25 acetate was used with different concentrations of acetic acid in 80% methanol:water (v/v). The separated constituents included the β-acids, α-acids, humulinic acids, iso-α-acids, and hulupones. Results obtained were comparable to those from gradient elution in accuracy and precision. Resolution was improved, particularly for the separation of β-acids from the less acidic unidentified substances. Stepwise elution eliminates the need for automated gradient elution equipment.

• Alpha-acids
• Beta-acids
• Hulupones
• Humulinic acids
• Iso-alpha-acids