Expression of Wild-Type α-Catenin Protein in Cells with a Mutant α-Catenin Gene Restores Both Growth Regulation and Tumor Suppressor Activities

Abstract

Recent studies indicate that disruption of the E-cadherin-mediated cell-cell adhesion system is frequently associated with human cancers of epithelial origin. Reduced levels of both E-cadherin and the associated protein, α-catenin, have been reported in human tumors. This report describes the characterization of a human ovarian carcinoma-derived cell line (Ov2008) which expresses a novel mutant form of the α-catenin protein lacking the extreme N terminus of the wild-type protein. The altered form of α-catenin expressed in Ov2008 cells fails to bind efficiently to β-catenin and is localized in the cytoplasm. Deletion mapping has localized the β-catenin binding site on α-catenin between amino acids 46 and 149, which encompasses the same region of the protein that is deleted in the Ov2008 variant. Restoration of inducible expression of the wild-type α-catenin protein in these cells caused them to assume the morphology typical of an epithelial sheet and retarded their growth in vitro. Additionally, the induction of α-catenin expression in Ov2008 cells injected into nude mice attenuated the ability of these cells to form tumors. These observations support the classification of α-catenin as a growth-regulatory and candidate tumor suppressor gene.