Abstract
Using a superoxide (O2 −) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG) was identified as an O2 − generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O2 − level in a dose-dependent manner with an IC50 value of 21 μM, comparable to those of rosmarinic acid (RoA, IC50=29 μM) and caffeic acid (CA, IC50=30 μM). While RoA and CA also dose-dependently inhibited O2 − generation in a xanthine-xanthine oxidase system, DLGG had no effect in the same system. Thus DLGG appeared to decrease the O2 − level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O2 − scavengers.