The 1-deoxynojirimycin (DNJ) biosynthetic gene cluster of Bacillus amyloliquefaciens 140N isolated from traditional Korean fermented food was isolated by PCR screening. It showed 78.9% inhibitory activity against α-glucosidase and produced 0.8 g/L of DNJ in an optimized medium containing 2% soluble starch, 1% tryptone, 0.05% KH2PO4, and 0.05% (NH2)4SO4. Soybean paste fermented with B. amyloliquefaciens 140N produced DNJ with 84.4% inhibitory activity.
Bioscience, Biotechnology, and Biochemistry
Volume 77, 2013 - Issue 2
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