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Abstract
Spiciferone A is a major phytotoxin produced by an isolate of Cochliobolus spicifer Nelson (D-5), a pathogen of leaf spot disease in wheat. In addition to spiciferone A, five of its derivatives, including spiciferones B and C, were tested for their phytotoxicity by using wheat cotyledon protoplasts. The results indicate that the ketone function at C-7 as well as the methyl at C-2 are essential to the phytotoxicity of spiciferone A.