Abstract
Human lactoferrin was produced in genetically engineered rice. N-linked glycan structures of recombinant human lactoferrin were determined. The oligosaccharides liberated by hydrazinolysis were labeled with 2-aminopyridine (PA). The PA-labeled glycans were purified by reverse-phase and size-fractionation HPLCs. The structures of these glycans were identified by HPLC, exoglycosidase digestion, and matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) mass spectrometry. The glycan structures determined were ManFucXylGlcNAc2 (3.4%), Man2FucGlcNAc2 (2.1%), Man3FucGlcNAc2 (2.5%), Man3FucXylGlcNAc2 (42.5%), two isomers of Man2FucXylGlcNAc2 (39.1%), Man3XylGlcNAc2 (6.5%), and Man2XylGlcNAc2 (3.9%).