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Abstract
Shoot multiplication was achieved from cotyledon and hypocotyl segments of in vitro germinated seedlings of Enterolobium cyxclocarpum on Murashige and Skoog's medium supplemented with BA (0.5-2.0 mg/L) and IAA (0.1-0.3 mg/L). Among the different concentrations and combination tested, BA (1.0 mg/L) in combination with IAA (0.2 mg/L) stimulated the best response in terms of shoot bud formation from cotyledon expiants in which a maximum of 60% culture responded, with a maximum of 2.7 ± 0.3 shoot buds per expiant with a mean shoot length of 2.0 ±0.2 cm. A maximum of 80% of the hypocotyl expiants exhibited such organogenic response when cultured on MS medium supplemented with 1.5 mg/L BA and 0.2 mg/L IAA with a mean shoot bud number of 4.1 ± 0.3 per expiant and a mean shoot length of 2.7 ±0.1 cm. Elongated shoots could be rooted on the half-strength MS basal medium supplemented with either IAA or IBA. Cotyledonary node expiants showed poor response.