Impact of tobacco smoking on cytokine signaling via interleukin-17A in the peripheral airways

Abstract

There is excessive accumulation of neutrophils in the airways in chronic obstructive pulmonary disease (COPD) but the underlying mechanisms remain poorly understood. It is known that extracellular cytokine signaling via interleukin (IL)-17A contributes to neutrophil accumulation in the airways but nothing is known about the impact of tobacco smoking on extracellular signaling via IL-17A. Here, we characterized the impact of tobacco smoking on extracellular cytokine signaling via IL-17A in the peripheral airways in long-term smokers with and without COPD and in occasional smokers before and after short-term exposure to tobacco smoke. We quantified concentrations of IL-17A protein in cell-free bronchoalveolar lavage (BAL) fluid samples (Immuno-quantitative PCR) and cytotoxic T-cells (immunoreactivity for CD8⁺ and CD3⁺) in bronchial biopsies. Matrix metalloproteinase-8 and human beta defensin 2 proteins were also quantified (enzyme-linked immunosorbent assay) in the BAL samples. The concentrations of IL-17A in BAL fluid were higher in long-term smokers without COPD compared with nonsmoking healthy controls, whereas those with COPD did not differ significantly from either of the other groups. Short-term exposure to tobacco smoke did not induce sustained alterations in these concentrations in occasional smokers. Long-term smokers displayed higher concentrations of IL-17A than did occasional smokers. Moreover, these concentrations correlated with CD8⁺ and CD3⁺ cells in biopsies among long-term smokers with COPD. In healthy nonsmokers, BAL concentrations of matrix metalloproteinase-8 and IL-17A correlated, whereas this was not the case in the pooled group of long-term smokers with and without COPD. In contrast, BAL concentrations of human beta defensin 2 and IL-17A correlated in all study groups. This study implies that long-term but not short-term exposure to tobacco smoke increases extracellular cytokine signaling via IL-17A in the peripheral airways. In the smokers with COPD, this signaling may involve cytotoxic T-cells. Long-term exposure to tobacco smoke leads to a disturbed association of extracellular IL-17A signaling and matrix metalloproteinase-8, of potential importance for the coordination of antibacterial activity.

Keywords:

• BAL
• COPD
• IL-17
• tobacco
• long-term smoking
• occasional smoking

Acknowledgments

The authors wish to acknowledge the excellent technical assistance by Ann-Sofie Lantz, BSc and Elisabeth Henriksson, BSc, and the skillful recruitment of study subjects by Maria Skedinger, MD and Anita Simhag, MD, all from Karolinska University Hospital. The authors also wish to acknowledge the skillful recruitment and investigation of study subjects by Britt-Marie Eldh from Sahlgrenska University Hospital. Project funding was obtained from the Swedish Research Council, the Swedish Heart-Lung Foundation, the Foundation for Rehabilitation and Medical Research in Gothenburg (FRF-stiftelsen), Stockholm County Council, Apotekare Hedbergs Foundation, King Gustaf V’s and Queen Victoria’s Freemason Research Fund, and Karolinska Institutet.

Disclosure

The authors report no conflicts of interest in this work.