https://orcid.org/0000-0003-3458-0528
Abstract
Purpose
TNF-α is a transmembrane protein which requires cleavage by ADAM17 in order to act systemically. The activation of ADAM17 to generate soluble TNF‑α results in an increased inflammatory activity. We hypothesized that variants spanning the ADAM17 gene contribute towards the observed variation in patient response defined by the number of changes in TNF‑α inhibitors.
Patients and Methods
Seven single-nucleotide polymorphisms (SNPs) of ADAM17 in 63 patients with rheumatoid arthritis who received TNF-α inhibitors were analyzed: rs57467365, rs62117540, rs117645314, rs6432013, rs532704607, rs117179141, and rs12692386. Univariate and multivariate regression analysis were employed to investigate the independent predictable factors for changes in TNF-α inhibitors.
Results
ADAM17 rs117645314 and rs117179141 showed significant association with the number of changes in TNF-α inhibitors. Patients with GA in rs117645314 and AT in rs117179141 had significantly higher chance of two or more changes of TNF-α inhibitors than those with wild homozygous alleles. Multivariate analysis showed that rs117179141 explained 5.7% of the 23.8% variability in TNF-α inhibitor response.
Conclusion
This study showed that the number of changes in TNF-α inhibitor is associated with ADAM17 SNPs.
Abbreviations
RA, rheumatoid arthritis; TNFi, tumor necrosis factor-α inhibitor; TACE, TNF-α converting enzyme; ADAM17, A disintegrin and metalloproteinase 17; SNPs, single-nucleotide polymorphisms; DAS, disease activity score; RF, rheumatoid factor; ACPA, anti-cyclic citrullinated peptide antibody; LD, linkage disequilibrium.
Disclosure
The authors report no conflicts of interest in this work.