Treatment of EAE Mice with Treg, G-MDSC and IL-2: a New Insight into Cell Therapy for Multiple Sclerosis

Abstract

Background: This study investigates the therapeutic and protective effects of Tregs, myeloid-derived suppressor cells (MDSCs) and IL-2 on multiple sclerosis (MS) disease model. Materials & methods: C57BL/6 mice were immunized to develop an experimental autoimmune encephalomyelitis (EAE) model. We then investigated effects of pre- and post-treatment EAE mice with Tregs, MDSCs and IL-2 on inflammation and demyelination in brain tissue, and on the number of Treg, granulocytic-MDSC and Th-17 cells in spleen. Results: Pre- and post-treatment of EAE mice by Tregs, MDSCs and IL-2 resulted in no weight change, reduced Th-17 cells and suppression of pathological properties. Conclusion: Pre- and post-treatment of immunized mice by Tregs, MDSCs and IL-2 prevent EAE induction.

Plain language summary

This study investigates the therapeutic and protective effects of suppressive immune cells and pivotal cytokines on multiple sclerosis disease model. In this study, mice were immunized to develop experimental autoimmune model. We then investigated effects of pre- and post-treatment model mice with suppressive immune cells and pivotal cytokines on immunomodulatory and pro-inflammatory cells. Pre- and post-treatment of model mice resulted in no weight change, reduced pro-inflammatory cells and suppression of undesired pathological properties.

Keywords::

• experimental autoimmune encephalomyelitis
• IL-2
• MDSC
• multiple sclerosis
• Treg cell

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/imt-2021-0045

Author contributions

GM Mehdi was responsible for laboratory experimentation, critical analysis and drafting and finalization of the manuscript. S Majid was responsible for concept design, supervision and finalization of the manuscript. F Touraj, A Saeid and H Hadi were responsible for process design optimization, laboratory experimentation, critical analysis and drafting and finalization of the manuscript. All authors read and approved the final manuscript.

Financial & competing interests disclosure

This work was supported by the Research and Technology Council of Golestan University of Medical Sciences (grant no. 960129001). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval for animal experimental investigations(N.IR.GOUMS.REC.1395.249). The animals used in this study were maintained in accordance with the ethical guidelines of Golestan University of Medical Sciences, Gorgan, Iran.

Additional information

Funding

This work was supported by the Research and Technology Council of Golestan University of Medical Sciences (grant no. 960129001). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.