Isolation and Characterization of an Undifferentiated Human Colon Carcinoma Cell Line (MIP-101)

Richard M. Niles Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Sally A. Wilhelm Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Glenn D. Steele Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Bohdana Burke Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Thomas Christensen Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Daniel Dexter Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Michael J. O'Brien Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Peter Thomas Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

Norman Zamcheck Mallory Gastrointestine Research Laboratory Boston City Hospital; Department of Biochemistry and Pathology, Boston University School of Medicine; Department of Surgery, New England Deaconess Hospital; Department of Medicine and Surgery, Harvard Medical School, Boston, Massachusetts; Department of Medicine, Brown University Providence, Rhode Island, ravi

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Abstract

An undifferentiated human colon carcinoma cell line was established from tumor tissue obtained from metastasis to the liver of colonic adenocarcinoma in a patient with fulminant Dukes D colorectal carcinoma. Histological analysis of the tumor biopsy from the liver confirmed the hospital pathology report of poorly differentiated colonic adenocarcinoma. Explants of this tumor tissue xenografted into a nude mouse were used to establish an epithelioid-like cell culture line, MIP-101. The cell line formed tumors in nude mice that hisologically appeared undifferentiated and did not stain for carcinoembryonic antigen (CEA). No CEA was present either by radio-immunoassay (RIA) of the culture supernatant or by immunoperoxidase staining of the tumors or monolayers. MIP-101 appears to be one of the most undifferentiated human colon carcinoma cells lines available. It should prove useful in the search for markers of undifferentiated colonic cancer and in studies of colonic cancer differentiation.

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Isolation and Characterization of an Undifferentiated Human Colon Carcinoma Cell Line (MIP-101)

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Isolation and Characterization of an Undifferentiated Human Colon Carcinoma Cell Line (MIP-101)

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