Enrichment of Residual Tumor Cells in Bone Marrow Or Peripheral Blood Cells in a Neuroectodermal Tumor Model

Abstract

To enrich a small population of malignant cells contaminating human bone marrow or peripheral blood, a method of immunomagnetic depletion of the normal nucleated cells was developed. In a model using neuroectodermal cell lanes, contaminations between 0.1% and 1.4% of tumor cells could be increased by a factor of 7.5 (median; range, 3.8 to 18.0; n = 10). Because incubation with antibodies is restricted to the magnetic beads in this method, th cell population after removal of the beads has not been incubated with antibodies. The risk of unspecific staining of the tumor cells during the enrichment procedure is minimal. This simple method is therefore well suited to the study of the characteristics of a minimal residual disease population.

Key Words:

• bone marrow
• enrichment of tumor cells
• neuroectodermal cell line
• peripheral blood
• residual disease