Processivity and substrate-binding in family 18 chitinases

Abstract

Enzymatic depolymerisation of polysaccharides is a key technology in the biorefining of biomass. The enzymatic conversion of the abundant insoluble polysaccharides cellulose and chitin is of particular interest and complexity, because of the bi-phasic nature of the process, the seemingly complicated tasks faced by the enzymes, and the importance of these conversions for the future biorefinery. Here we review recent work on family 18 chitinases that sheds light on important aspects of the catalytic action of these depolymerising enzymes, including the structural basis of processivity and its direction, the energies involved in substrate-binding and displacement.

Keywords::

• Processivity
• glycoside hydrolase
• substrate-binding
• chitinase

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.