Association Analysis about HLA-DRB1, -DQB1 Polymorphism and Auto-Antibodies Against α₁-Adrenergic Receptors in Chinese Patients with Essential Hypertension

Abstract

The auto-antibodies against α₁-adrenergic receptors (α₁-AAs) with agonist activity likes norepinephrine have been discovered in patients with essential hypertension but the mechanism of α₁-AA production remains unclear. We supposed the α₁-AAs be correlated to the HLA-DQB1 and DRB1 alleles. Three hundred ninety-six patients with essential hypertension (EH) and 224 normotensives were enrolled, and DNA typing was detected by protein coupled receptor (PCR) amplification with sequence-specific primers. Analysis was performed by α² and logistic regression. There were the significant associations of the haplotype HLA-DQB1**0301-DRB1**04 with the prevalence of α₁-AAs in hypertensive patients and it obviously added to the risk for the α₁-AA production (adjusted P == 0.019, OR 4.037, 95%% CI 1.259–12.947). In normotensives, the haplotype HLA-DQB1**05-DRB1**04 provided a strong predisposition in α ₁-AAs production (adjusted P == 0.024, OR 3.922, 95%% CI 1.200–12.817). These results suggest the HLA-DRB1**04 might be the primary risk alleles associated with α₁-AA production on the haplotypes HLA-DQB1**0301-DRB1**04 and HLA‐‐DQB1**05-DRB1**04 and increased the risk for α ₁-AA production.

• auto-antibody
• hypertension
• HLA-DRB1
• HLA-DQB1
• polymorphism

ACKNOWLEDGMENT

This study was supported by grants from National Nature Science Foundation of China (No. 030600235).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.