Nanomaterials as Matrices for Enzyme Immobilization

Abstract

Nanomaterials constitute novel and interesting matrices for enzyme immobilization. While their high surface to volume ratio is an obvious advantage, their Brownian motion can impact the behavior of enzymes immobilized on these matrices. Carbon nanotubes, superparamagnetic nanoparticles, and mesoporous materials constitute some important classes of matrices. Such immobilized enzyme systems have been used in both aqueous and low water media for biocatalysis and resolution of racemates. This overview examines the behavior of enzymes immobilized on nanomaterials and discusses the results reported with such biocatalyst preparations.

Keywords::

• superparamagnetic nanoparticles
• carbon nanotubes
• enzyme immobilization
• enantioselective biocatalysis
• enzyme catalysis in low water media
• quantum dots
• mesoporous materials
• protein refolding

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.