Nutritive Medium Engineering Enhanced Production of Extracellular Lipase by Trichoderma Longibrachiatum

ABSTRACT

The influence of six different plant oils, oleic acid, linoleic acid and different nitrogen sources on the production of extracellular lipase by Trichoderma longibrachiatum in submerged fermentation was studied. It was obtained that enzyme production strongly depended on the nutritive medium composition. The decreasing of oleic acid content and increasing of linoleic acid content decreased the production of extracellular lipase by Trichoderma longibrachiatum. Inorganic nitrogen sources, in particular (NH₄)₂SO₄, stimulated lipase production. As a result of the applied mathematical modeling and optimization procedure the optimal nutritive medium composition was determined. In the optimized nutritive medium maximum lipase activity 13.98 U-cm⁻³ was reached at 72 h, which corresponded to the end of the exponential growth phase. As a result of the applied optimization procedures almost 2.5 times increasing of lipase production was achieved in comparison with basal medium.

Keywords:

• lipase
• mathematical modeling
• optimization
• plant oils
• Trichoderma longibrachiatum