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Original Articles

Purification of avian circulating primordial germ cells by Nycodenz density gradient centrifugation

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Pages 30-35 | Published online: 28 Jun 2010

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (3)

B. Benesova & P. Trefil. (2016) Possibilities for preserving genetic resources in birds. World's Poultry Science Journal 72:3, pages 629-642.
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M. Naito, T. Harumi, T. Minematsu, A. Tajima & T. Kuwana. (2007) Effect of soft X-ray irradiation on the migratory ability of primordial germ cells in chickens. British Poultry Science 48:2, pages 121-126.
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Articles from other publishers (53)

Kornkanok Sritabtim, Sukumal Prukudom, Yanika Piyasanti, Suparat Chaipipat, Takashi Kuwana, Juthathip Jurutha, Rungthiwa Sinsiri, Chanin Tirawattanawanich & Kannika Siripattarapravat. (2024) First study on repeatable culture of primordial germ cells from various embryonic regions with giant feeder cells in Japanese quail (Coturnix japonica). Theriogenology 213, pages 43-51.
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Luiza Chojnacka-Puchta & Dorota Sawicka. 2024. Germ Cell Development. Germ Cell Development 15 25 .
Afsaneh Golkar‐Narenji, Piotr Dziegiel, Bartosz Kempisty, James Petitte, Paul Edward Mozdziak & Artur Bryja. (2023) In vitro culture of reptile PGCS to preserve endangered species. Cell Biology International 47:8, pages 1314-1326.
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Young-Min Kim, Seung-Je Woo & Jae-Yong Han. (2023) Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications. Genes 14:4, pages 899.
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András Ecker, Bence Lázár, Roland Imre Tóth, Martin Urbán, Nikolett Tokodyné Szabadi, Maria Teresa Salinas Aponte, Mohd Adnan, Eszter Várkonyi & Elen Gócza. (2023) The Effects of Freezing Media on the Characteristics of Male and Female Chicken Primordial Germ Cell Lines. Life 13:4, pages 867.
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Natsuko HAMAI, Chihiro KOIDE, Yuki TANSHO, Yukino OOKA, Mayo HIRANO, Effrosyni FATIRA, Masaoki TSUDZUKI & Yoshiaki NAKAMURA. (2023) Development of cryopreservation media for the slow-freezing of cultured primordial germ cells in chicken. Journal of Reproduction and Development 69:2, pages 109-117.
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Stefanie Altgilbers, Sabine Klein, Claudia Dierks, Steffen Weigend & Wilfried A. Kues. (2021) Cultivation and characterization of primordial germ cells from blue layer hybrids (Araucana crossbreeds) and generation of germline chimeric chickens. Scientific Reports 11:1.
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Su-Hyeon Kim, Heeil Do, Gyeongjun Cho, Da-Ran Kim & Youn-Sig Kwak. (2021) Bacterial Community Structure and the Dominant Species in Imported Pollens for Artificial Pollination. The Plant Pathology Journal 37:3, pages 299-306.
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Qisheng Zuo, Kai Jin, Man Wang, Yani Zhang, Guohong Chen & Bichun Li. (2021) BMP4 activates the Wnt– Lin28A – Blimp1 –Wnt pathway to promote primordial germ cell formation via altering H3K4me2 . Journal of Cell Science 134:3, pages jcs249375.
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Rie Goto & Taiju Saito. (2019) A state-of-the-art review of surrogate propagation in fish. Theriogenology 133, pages 216-227.
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Fuxian Yu, Zhiwei Zhu, Xiaoyu Chen, Jing Huang, Ruoxin Jia & Jianzhi Pan. (2019) Isolation, characterization and germline chimera preparation of primordial germ cells from the Chinese Meiling chicken. Poultry Science 98:2, pages 566-572.
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Jae Yong Han & Young Hyun Park. (2018) Primordial germ cell-mediated transgenesis and genome editing in birds. Journal of Animal Science and Biotechnology 9:1.
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Young Sun Hwang & Jae Yong Han. 2018. Application of Genetics and Genomics in Poultry Science. Application of Genetics and Genomics in Poultry Science.
Marek Bednarczyk, Izabela Kozłowska, Paweł Łakota, Agata Szczerba, Katarzyna Stadnicka & Takashi Kuwana. (2018) Generation of transgenic chickens by the non-viral, cell-based method: effectiveness of some elements of this strategy. Journal of Applied Genetics 59:1, pages 81-89.
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Kyung M. Jung, Young M. Kim, Tamao Ono & Jae Y. Han. (2017) Size-dependent isolation of primordial germ cells from avian species. Molecular Reproduction and Development 84:6, pages 508-516.
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Yoshiaki Nakamura. 2017. Avian Reproduction. Avian Reproduction 187 214 .
Yoshiaki NAKAMURA. (2016) Poultry genetic resource conservation using primordial germ cells. Journal of Reproduction and Development 62:5, pages 431-437.
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. 2016. Avian Medicine. Avian Medicine 522 566 .
Mitsuru Naito, Takashi Harumi & Takashi Kuwana. (2015) Long-term culture of chicken primordial germ cells isolated from embryonic blood and production of germline chimaeric chickens. Animal Reproduction Science 153, pages 50-61.
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L. Yan, J.S. Gao, L. Rui, Y.N. Lu, Z.Y. Wan, X.X. Yu, W.X. Zhang, H.T. Li, Q. Shao, J. Zhang & Z.D. Li. (2014) A Review of Strategies for Producing Chimeric Birds. Avian Biology Research 7:1, pages 3-9.
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Kazuhiro Rikimaru, Yoshiaki Nakamura, Daiki Takahashi, Megumi Komatsu, Natsuki Ito, Kazuei Matsubara & Takahiro Tagami. (2014) Production of Pure Hinai-dori with Normal Reproductive Capability from Transferred Primordial Germ Cells. The Journal of Poultry Science 51:3, pages 297-306.
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Daichi Miyahara, Takafumi Mori, Ryuichi Makino, Yoshiaki Nakamura, Isao Oishi, Tamao Ono, Keijiro Nirasawa, Takahiro Tagami & Hiroshi Kagami. (2014) Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells. The Journal of Poultry Science 51:1, pages 87-95.
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Atsushi Tajima. (2013) Conservation of Avian Genetic Resources. The Journal of Poultry Science 50:1, pages 1-8.
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Yoshiaki NAKAMURA, Mariko TASAI, Kumiko TAKEDA, Keijiro NIRASAWA & Takahiro TAGAMI. (2013) Production of Functional Gametes from Cryopreserved Primordial Germ Cells of the Japanese Quail. Journal of Reproduction and Development 59:6, pages 580-587.
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Ken‐ichi Nishijima & Shinji Iijima. (2012) Transgenic chickens. Development, Growth & Differentiation 55:1, pages 207-216.
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Yoshiaki Nakamura, Hiroshi Kagami & Takahiro Tagami. (2013) Development, differentiation and manipulation of chicken germ cells. Development, Growth & Differentiation 55:1, pages 20-40.
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Michael J. McGrew. 2013. Sustainable Food Production. Sustainable Food Production 236 249 .
Ana De Melo Bernardo, Kaylee Sprenkels, Gabriela Rodrigues, Toshiaki Noce & Susana M. Chuva De Sousa Lopes. (2012) Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation. Biology Open 1:11, pages 1146-1152.
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Yoshiaki NAKAMURA, Fumitake USUI, Daichi MIYAHARA, Takafumi MORI, Tamao ONO, Hiroshi KAGAMI, Kumiko TAKEDA, Keijiro NIRASAWA & Takahiro TAGAMI. (2012) X-irradiation Removes Endogenous Primordial Germ Cells (PGCs) and Increases Germline Transmission of Donor PGCs in Chimeric Chickens. Journal of Reproduction and Development 58:4, pages 432-437.
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Michael J. McGrew. 2012. Encyclopedia of Sustainability Science and Technology. Encyclopedia of Sustainability Science and Technology 638 652 .
Takahiro Tagami & Yoshiaki Nakamura. (2019) Improving Rates of Germline Transmission in Chimeric Chickens Using Busulfan Solubilised Sustained-Release Emulsion. Avian Biology Research 4:2, pages 74-77.
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Manabu Onuma & Takashi Kuwana. (2011) Preliminary Research on Mycoplasma synoviae Vertical Transmission Rate into Primordial Germ Cells. The Journal of Poultry Science 48:3, pages 212-215.
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Yoshiaki Nakamura, Fumitake Usui, Daichi Miyahara, Takafumi Mori, Haruhi Watanabe, Tamao Ono, Kumiko Takeda, Keijiro Nirasawa, Hiroshi Kagami & Takahiro Tagami. (2011) Viability and Functionality of Primordial Germ Cells after Freeze-thaw in Chickens. The Journal of Poultry Science 48:1, pages 57-63.
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Yanfei Yang & Ali Honaramooz. (2011) Efficient purification of neonatal porcine gonocytes with Nycodenz and differential plating. Reproduction, Fertility and Development 23:3, pages 496.
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Yoshiaki Nakamura, Fumitake Usui, Tamao Ono, Kumiko Takeda, Keijiro Nirasawa, Hiroshi Kagami & Takahiro Tagami. (2010) Germline Replacement by Transfer of Primordial Germ Cells into Partially Sterilized Embryos in the Chicken1. Biology of Reproduction 83:1, pages 130-137.
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Makoto Motono, Yuki Yamada, Yuki Hattori, Ryo Nakagawa, Ken-ichi Nishijima & Shinji Iijima. (2010) Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector. Journal of Bioscience and Bioengineering 109:4, pages 315-321.
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Mitsuru Naito, Takashi Harumi & Takashi Kuwana. (2010) Long Term in vitro Culture of Chicken Primordial Germ Cells Isolated from Embryonic Blood and Incorporation into Germline of Recipient Embryo. The Journal of Poultry Science 47:1, pages 57-64.
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Yoshiaki Nakamura, Fumitake Usui, Daichi Miyahara, Takafumi Mori, Tamao Ono, Kumiko Takeda, Keijiro Nirasawa, Hiroshi Kagami & Takahiro Tagami. (2010) Efficient system for preservation and regeneration of genetic resources in chicken: concurrent storage of primordial germ cells and live animals from early embryos of a rare indigenous fowl (Gifujidori). Reproduction, Fertility and Development 22:8, pages 1237.
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Yoshiaki Nakamura, Hiroshi Kagami & Takahiro Tagami. (2009) . The Journal of Animal Genetics 37:1, pages 41-57.
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Mitsuru Naito, Takeo Minematsu, Takashi Harumi & Takashi Kuwana. (2009) Preferential Migration of Transferred Primordial Germ Cells to Left Germinal Ridge of Recipient Embryos in Chickens. The Journal of Poultry Science 46:1, pages 40-45.
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Yoshiaki Nakamura, Fumitake Usui, Yusuke Atsumi, Asako Otomo, Ayumi Teshima, Tamao Ono, Kumiko Takeda, Keijiro Nirasawa, Hiroshi Kagami & Takahiro Tagami. (2009) Effects of Busulfan Sustained-release Emulsion on Depletion and Repopulation of Primordial Germ Cells in Early Chicken Embryos. The Journal of Poultry Science 46:2, pages 127-135.
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Susumu ISHIGURO, Takeo MINEMATSU, Mitsuru NAITO, Yukio KANAI & Atsushi TAJIMA. (2009) Migratory Ability of Chick Primordial Germ Cells Transferred into Quail Embryos. Journal of Reproduction and Development 55:2, pages 183-186.
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Takaharu Kawashima, Urszula Stępińska, Takashi Kuwana & BoŻenna Olszańska. (2008) Melatonin receptor genes (mel‐1a, mel‐1b, mel‐1c) are differentially expressed in the avian germ line. Molecular Reproduction and Development 75:9, pages 1408-1417.
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Yoshiaki Nakamura, Yasuhiro Yamamoto, Fumitake Usui, Yusuke Atsumi, Yohei Ito, Tamao Ono, Kumiko Takeda, Keijiro Nirasawa, Hiroshi Kagami & Takahiro Tagami. (2008) Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens. Reproduction, Fertility and Development 20:8, pages 900.
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Y. Nakamura, Y. Yamamoto, F. Usui, T. Mushika, T. Ono, A.R. Setioko, K. Takeda, K. Nirasawa, H. Kagami & T. Tagami. (2007) Migration and Proliferation of Primordial Germ Cells in the Early Chicken Embryo. Poultry Science 86:10, pages 2182-2193.
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Yasuhiro Yamamoto, Fumitake Usui, Yoshiaki Nakamura, Yohei Ito, Takahiro Tagami, Keijiro Nirasawa, Yuko Matsubara, Tamao Ono & Hiroshi Kagami. (2007) A Novel Method to Isolate Primordial Germ Cells and Its Use for the Generation of Germline Chimeras in Chicken1. Biology of Reproduction 77:1, pages 115-119.
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Argono R. Setioko, Takahiro TagamiHideki TaseYoshiaki Nakamura, Kumiko TakedaKeijiro Nirasawa. (2007) Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants. The Journal of Poultry Science 44:1, pages 73-77.
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Mitsuru Naito, Takeo Minematsu, Takashi Harumi & Takashi Kuwana. (2007) Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells <I>in vitro</I> and <I>in vivo</I>. The Journal of Poultry Science 44:4, pages 416-425.
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D.T. Moore, P.H. Purdy & H.D. Blackburn. (2006) A Method for Cryopreserving Chicken Primordial Germ Cells. Poultry Science 85:10, pages 1784-1790.
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J.N. Petitte. (2006) Avian Germplasm Preservation: Embryonic Stem Cells or Primordial Germ Cells?. Poultry Science 85:2, pages 237-242.
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Takaharu Kawashima, Koji Kumata, Yoshinori Tamaki, Takashi Kuwana & Koichiro Hashimoto. (2005) An avian embryo culture system for embryogenesis using an artificial vessel: possible conservation benefits in the rescue and management of endangered avian species. Zoo Biology 24:6, pages 519-529.
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P.E. Mozdziak, J. Angerman-Stewart, B. Rushton, S.L. Pardue & J.N. Petitte. (2005) Isolation of chicken primordial germ cells using fluorescence-activated cell sorting. Poultry Science 84:4, pages 594-600.
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Mitsuru Naito, Takashi Harumi, Yuko Matsubara & Takashi Kuwana. (2005) An Attempt at Fusing Primordial Germ Cell with Embryonic Blood Cell in Chickens Using Inactivated Sendai Virus or Electrical Stimulation. The Journal of Poultry Science 42:4, pages 369-374.
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