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Original Articles

Culture of Bacillus larvae from bulk honey samples for the detection of American foulbrood

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Pages 13-16 | Received 19 Oct 1990, Accepted 28 Jun 1991, Published online: 24 Mar 2015

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (17)

Beqë Hulaj, Izedin Goga, Armend Cana, Xhavit Merovci, Franca Rossi, Simone Crudele, Luciano Ricchiuti & Franco Mutinelli. (2023) Passive surveillance of American foulbrood in the Republic of Kosovo: geographic distribution and genotype characterization. Journal of Apicultural Research 62:2, pages 320-325.
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Stefano Bassi, Giovanni Formato, Marcella Milito, Karin Trevisiol, Cristian Salogni & Elena Carra. (2015) Phenotypic characterization and ERIC–PCR based genotyping of Paenibacillus larvae isolates recovered from American foulbrood outbreaks in honey bees from Italy. Veterinary Quarterly 35:1, pages 27-32.
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Dirk C de Graaf, Adriana M Alippi, Karina Antúnez, Katherine A Aronstein, Giles Budge, Dieter De Koker, Lina De Smet, Douglas W Dingman, Jay D Evans, Leonard J Foster, Anne Fünfhaus, Eva Garcia-Gonzalez, Aleš Gregore, Hannelie Human, K Daniel Murray, Bach Kim Nguyen, Lena Poppinga, Marla Spivak, Dennis van Engelsdorp, Selwyn Wilkins & Elke Genersch. (2013) Standard methods for American foulbrood research. Journal of Apicultural Research 52:1, pages 1-28.
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Norman L Carreck. (2011) Fifty years of the Journal of Apicultural Research. Journal of Apicultural Research 50:4, pages 249-256.
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LieselB. Gende, MatiasD. Maggi, Rosalía Fritz, MartinJ. Eguaras, PedroN. Bailac & MartaI. Ponzi. (2009) Antimicrobial Activity of Pimpinella anisum and Foeniculum vulgare Essential Oils Against Paenibacillus larvae . Journal of Essential Oil Research 21:1, pages 91-93.
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Adriana M Alippi, Francisco J Reynaldi, Ana C López, Marisa R De Giusti & O Mario Aguilar. (2004) Molecular epidemiology of Paenibacillus larvae larvae and incidence of American foulbrood in Argentinean honeys from Buenos Aires province. Journal of Apicultural Research 43:3, pages 135-143.
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M L Del Hoyo, M Basualdo, A Lorenzo, M A Palacio, E M Rodriguez & E Bedascarrasbure. (2001) Effect of shaking honey bee colonies affected by American foulbrood on Paenibacillus larvae larvae spore loads. Journal of Apicultural Research 40:2, pages 65-69.
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Dulce Maria Tocchetto Schuch, Robert Heaviside Madden & Aroni Sattler. (2001) An improved method for the detection and presumptive identification of Paenibacillus larvae subsp. larvae spores in honey. Journal of Apicultural Research 40:2, pages 59-64.
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Camilla J Brødsgaard, Henrik Hansen & Wolfgang Ritter. (2000) Progress of Paenibacillus larvae larvae infection in individually inoculated honey bee larvae reared singly in vitro, in micro colonies, or in full-size colonies. Journal of Apicultural Research 39:1-2, pages 19-27.
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Henrik Hansen & Camilla Juul Brødsgaard. (1999) American foulbrood: a review of its biology, diagnosis and control. Bee World 80:1, pages 5-23.
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Michael A Z Hornitzky & Lisa Smith. (1998) Procedures for the Culture of Melissococcus Pluton from Diseased Brood and Bulk Honey Samples. Journal of Apicultural Research 37:4, pages 293-294.
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Denis Anderson, Helen Giacon & Nicole Gibson. (1997) Detection and thermal destruction of the chalkbrood fungus (Ascosphaera apis) in honey. Journal of Apicultural Research 36:3-4, pages 163-168.
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Susann Nordström & Ingemar Fries. (1995) A comparison of media and cultural conditions for identification of Bacillus larvae in honey. Journal of Apicultural Research 34:2, pages 97-103.
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Steven Djordjevic, Michael Ho-Shon & Michael Hornitzky. (1994) DNA restriction endonuclease profiles and typing of geographically diverse isolates of Bacillus larvae. Journal of Apicultural Research 33:2, pages 95-103.
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M A Z Hornitzky & P J Nicholls. (1993) J Medium is Superior to Sheep Blood Agar and Brain Heart Infusion Agar for the Isolation of Bacillus Larvae from Honey Samples. Journal of Apicultural Research 32:1, pages 51-52.
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Articles from other publishers (24)

Elena Carra, Giorgio Galletti, Emanuele Carpana, Federica Bergamini, Giulio Loglio, Filippo Bosi, Stefano Palminteri & Stefano Bassi. (2022) A Probe-Based qPCR Method, Targeting 16S rRNA Gene, for the Quantification of Paenibacillus larvae Spores in Powdered Sugar Samples. Applied Sciences 12:19, pages 9895.
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Michael W. Zabrodski, Tasha Epp, Geoff Wilson, Igor Moshynskyy, Mohsen Sharafi, Lara Reitsma, Mateo Castano Ospina, Jessica E. DeBruyne, Alexandra Wentzell, Sarah C. Wood, Ivanna V. Kozii, Colby D. Klein, Jenna Thebeau, LaRhonda Sobchishin, Antonio C. Ruzzini & Elemir Simko. (2022) Establishment of apiary-level risk of American foulbrood through the detection of Paenibacillus larvae spores in pooled, extracted honey in Saskatchewan. Scientific Reports 12:1.
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Stefano Bassi, Giorgio Galletti, Emanuele Carpana, Stefano Palminteri, Filippo Bosi, Giulio Loglio & Elena Carra. (2022) Powdered Sugar Examination as a Tool for the Assessment of Paenibacillus larvae Infection Levels in Honey Bee Colonies. Frontiers in Veterinary Science 9.
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Michael W. Zabrodski, Jessica E. DeBruyne, Geoff Wilson, Igor Moshynskyy, Mohsen Sharafi, Sarah C. Wood, Ivanna V. Kozii, Jenna Thebeau, Colby D. Klein, Igor Medici de Mattos, LaRhonda Sobchishin, Tasha Epp, Antonio C. Ruzzini & Elemir Simko. (2022) Comparison of individual hive and apiary-level sample types for spores of Paenibacillus larvae in Saskatchewan honey bee operations. PLOS ONE 17:2, pages e0263602.
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Nur BOZBEYOGLU, Seher ARSLAN, Aykut GUVENSEN & Nazime MERCAN DOGAN. (2019) Bacteriological, Physicochemical, and Melissopalynologic Properties of Some Turkish HoneysBazı Türk Ballarının Bakteriyolojik, Fizikokimyasal ve Melissopalinolojik Analizi. Akademik Gıda 17:2, pages 167-175.
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Linde Morawetz, Hemma Köglberger, Antonia Griesbacher, Irmgard Derakhshifar, Karl Crailsheim, Robert Brodschneider & Rudolf Moosbeckhofer. (2019) Health status of honey bee colonies (Apis mellifera) and disease-related risk factors for colony losses in Austria. PLOS ONE 14:7, pages e0219293.
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Mohammad Javed Ansari, Ahmad Al-Ghamdi, Adgaba Nuru, Ashraf Mohamed Ahmed, Tahany H. Ayaad, Khalid Ali Khan & Noori Al-Waili. (2017) Diagnosis and molecular detection of Paenibacillus larvae, the causative agent of American foulbrood in honey bees in Saudi Arabia. International Journal of Tropical Insect Science 37:03, pages 137-148.
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Bryan D Merrill, Julianne H Grose, Donald P Breakwell & Sandra H Burnett. (2014) Characterization of Paenibacillus larvae bacteriophages and their genomic relationships to firmicute bacteriophages. BMC Genomics 15:1.
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Ignazio Floris, Emanuele Carpana, Stefano Bassi, Giovanni Formato, Antonella Cersini & Marco Lodesani. 2014. Patologia e avversità dell’alveare. Patologia e avversità dell’alveare 49 121 .
Stefano Bassi, Elena Carra, Emanuele Carpana, Gian Luca Paganelli & Stefano Pongolini. (2010) A scientific note on the detection of spores of Paenibacillus larvae in naturally and artificially contaminated honey: comparison of cultural and molecular methods . Apidologie 41:4, pages 425-427.
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Dirk C. de Graaf, Wolfgang Ritter, Frans J. Jacobs, Marleen Brunain, Hein Imberechts, Koen Mintiens, Yves Van der Stede, Bart Verheyden, Aud Kari Fauske, Patrick Boujon, Gabriela Chioveanu, Daniel Dezmirean, Giovanni Formato, Franco Mutinelli, Hendrik-Jan Roest, Dalibor Titĕra, Stephen F. Pernal & Katia Knapen. (2009) Lessons from the first international proficiency test for the detection of spores from the honey bee pathogen Paenibacillus larvae. Accreditation and Quality Assurance 14:5, pages 273-276.
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Anders Lindström, Seppo Korpela & Ingemar Fries. (2008) The distribution of Paenibacillus larvae spores in adult bees and honey and larval mortality, following the addition of American foulbrood diseased brood or spore-contaminated honey in honey bee (Apis mellifera) colonies. Journal of Invertebrate Pathology 99:1, pages 82-86.
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M. Gillard, J.D. Charriere & L. Belloy. (2008) Distribution of Paenibacillus larvae spores inside honey bee colonies and its relevance for diagnosis. Journal of Invertebrate Pathology 99:1, pages 92-95.
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Eva Forsgren, Jevrosima Stevanovic & Ingemar Fries. (2008) Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes. Veterinary Microbiology 129:3-4, pages 342-349.
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D.C. de Graaf, A.M. Alippi, M. Brown, J.D. Evans, M. Feldlaufer, A. Gregorc, M. Hornitzky, S.F. Pernal, D.M.T. Schuch, D. Titĕra, V. Tomkies & W. Ritter. (2006) Diagnosis of American foulbrood in honey bees: a synthesis and proposed analytical protocols. Letters in Applied Microbiology 43:6, pages 583-590.
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Miriam O. Iurlina, Amelia I. Saiz, Sandra R. Fuselli & Rosalia Fritz. (2006) Prevalence of Bacillus spp. in different food products collected in Argentina. LWT - Food Science and Technology 39:2, pages 105-110.
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Xi-Yang Wu, Mark J. Walker, Michael Hornitzky & James Chin. (2006) Development of a group-specific PCR combined with ARDRA for the identification of Bacillus species of environmental significance. Journal of Microbiological Methods 64:1, pages 107-119.
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Miriam O. Iurlina & Rosalia Fritz. (2005) Characterization of microorganisms in Argentinean honeys from different sources. International Journal of Food Microbiology 105:3, pages 297-304.
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G. A. L. Broggini, B. Duffy, E. Holliger, H. -J. Sch�rer, C. Gessler & A. Patocchi. (2005) Detection of the fire blight biocontrol agent Bacillus subtilis BD170 (Biopro�) in a Swiss apple orchard. European Journal of Plant Pathology 111:2, pages 93-100.
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Tamás Bakonyi, Irmgard Derakhshifar, Elvira Grabensteiner & Norbert Nowotny. (2003) Development and Evaluation of PCR Assays for the Detection of Paenibacillus larvae in Honey Samples: Comparison with Isolation and Biochemical Characterization . Applied and Environmental Microbiology 69:3, pages 1504-1510.
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U. Riessberger-Gallé, W. von der Ohe & K. Crailsheim. (2001) Adult Honeybee's Resistance against Paenibacillus larvae larvae, the Causative Agent of the American Foulbrood. Journal of Invertebrate Pathology 77:4, pages 231-236.
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V. A. Govan, M. H. Allsopp & S. Davison. (1999) A PCR Detection Method for Rapid Identification of Paenibacillus larvae. Applied and Environmental Microbiology 65:5, pages 2243-2245.
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Rekha S Singhal, Pushpa R Kulkarni & Dinanath V Rege. 1997. Handbook of Indices of Food Quality and Authenticity. Handbook of Indices of Food Quality and Authenticity 358 385 .
K. H. Steinkraus & R. A. Morse. (2004) Media for the detection of Bacillus larvae spores in honey . Acta Biotechnologica 16:1, pages 57-64.
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