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Research Article

Isolation of monocytes from whole blood by density gradient centrifugation and counter-current elutriation followed by cryopreservation: six years' experience

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Pages 357-366 | Published online: 08 Jul 2009

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Yu Li, Yaju Deng & Jie He. (2021) Monocyte-related gene biomarkers for latent and active tuberculosis. Bioengineered 12:2, pages 10799-10811.
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Elisabeth Øya, Anita Solhaug, Anette K. Bølling, Reidun Øvstebø, Tonje B. Steensen, Anani K.J. Afanou & Jørn A. Holme. (2019) Pro-inflammatory responses induced by A. fumigatus and A. versicolor in various human macrophage models. Journal of Toxicology and Environmental Health, Part A 82:8, pages 483-501.
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Beate Vestad, Alicia Llorente, Axl Neurauter, Santosh Phuyal, Bente Kierulf, Peter Kierulf, Tore Skotland, Kirsten Sandvig, Kari Bente F. Haug & Reidun Øvstebø. (2017) Size and concentration analyses of extracellular vesicles by nanoparticle tracking analysis: a variation study. Journal of Extracellular Vesicles 6:1.
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Per Kr. Lund, Reidun Øvstebø, Anne‐Sophie W. Møller, Ole Kristoffer Olstad, Kirsti S. Landsverk, Marit Hellum & Peter Kierulf. (2009) Using global gene expression patterns to characterize Annexin V positive and negative human monocytes in culture. Scandinavian Journal of Clinical and Laboratory Investigation 69:2, pages 251-264.
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Ernst K. Rødland, Morten Mattingsdal, Ole K. Olstad, Reidun Øvstebø, Peter Kierulf, Fredrik Műller & Stig S. Frøland. (2008) Expression of genes in normal human monocytes in response to Aspergillus fumigatus. Medical Mycology 46:4, pages 327-336.
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Articles from other publishers (22)

Tonje Bjørnetrø, Lilly Alice Steffensen, Beate Vestad, Berit Sletbakk Brusletto, Ole Kristoffer Olstad, Anne‐Marie Trøseid, Hans Christian Dalsbotten Aass, Kari Bente Foss Haug, Alicia Llorente, Stig Ove Bøe, Anna Lång, Rampradeep Samiappan, Kathrine Røe Redalen, Reidun Øvstebø & Anne Hansen Ree. (2021) Uptake of circulating extracellular vesicles from rectal cancer patients and differential responses by human monocyte cultures. FEBS Open Bio 11:3, pages 724-740.
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Lidija Gradišnik, Marko Milojević, Tomaž Velnar & Uroš Maver. (2020) Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood. Molecular Biology Reports 47:9, pages 6929-6940.
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E. Øya, F.D. Zegeye, A.K. Bølling, R. Øvstebø, A.K.J. Afanou, J. Øvrevik, M. Refsnes & J.A. Holme. (2019) Hyphae fragments from A. fumigatus sensitize lung cells to silica particles (Min-U-Sil): Increased release of IL-1β. Toxicology in Vitro 55, pages 1-10.
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Unni Gopinathan, Kathrine Røe Redalen, Anne-Marie Trøseid, Peter Kierulf, Petter Brandtzaeg, Anne Hansen Ree, Jens Petter Berg & Reidun Øvstebø. (2018) Large-scale reduction of tyrosine kinase activities in human monocytes stimulated in vitro with N. meningitidis. PLOS ONE 13:1, pages e0181912.
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Marit Hellum, Anne-Marie S Trøseid, Jens P Berg, Petter Brandtzaeg, Reidun Øvstebø & Carola E Henriksson. (2016) The Neisseria meningitidis lpxL1 mutant induces less tissue factor expression and activity in primary human monocytes and monocyte-derived microvesicles than the wild type meningococcus . Innate Immunity 23:2, pages 196-205.
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Tyler A. Allen, David Gracieux, Maliha Talib, Debra A. Tokarz, M. Taylor Hensley, Jhon Cores, Adam Vandergriff, Junnan Tang, James B.M. de Andrade, Phuong-Uyen Dinh, Jeffrey A. Yoder & Ke Cheng. (2017) Angiopellosis as an Alternative Mechanism of Cell Extravasation. Stem Cells 35:1, pages 170-180.
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Unni Gopinathan, Berit Sletbakk Brusletto, Ole Kristoffer Olstad, Peter Kierulf, Jens Petter Berg, Petter Brandtzaeg & Reidun Øvstebø. (2014) IL-10 immunodepletion from meningococcal sepsis plasma induces extensive changes in gene expression and cytokine release in stimulated human monocytes. Innate Immunity 21:4, pages 429-449.
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Faith HA Osier, Gaoqian Feng, Michelle J Boyle, Christine Langer, Jingling Zhou, Jack S Richards, Fiona J McCallum, Linda Reiling, Anthony Jaworowski, Robin F Anders, Kevin Marsh & James G Beeson. (2014) Opsonic phagocytosis of Plasmodium falciparummerozoites: mechanism in human immunity and a correlate of protection against malaria. BMC Medicine 12:1.
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Erik K. Amundsen, Petter Urdal, Mette R. Holthe & Carola E. Henriksson. (2014) Aggregation of monocytes and platelets interferes in measurement of monocyte viability with phosphatidylserine expression but not with mitochondrial membrane potential in whole blood. Cytometry Part B: Clinical Cytometry, pages n/a-n/a.
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Unni Gopinathan, Reidun Øvstebø, Ole Kristoffer Olstad, Berit Brusletto, Hans Christian Dalsbotten Aass, Peter Kierulf, Petter Brandtzaeg & Jens Petter Berg. (2012) Global Effect of Interleukin-10 on the Transcriptional Profile Induced by Neisseria meningitidis in Human Monocytes. Infection and Immunity 80:11, pages 4046-4054.
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Reidun Øvstebø, Hans C Dalsbotten Aass, Kari Bente Foss Haug, Anne-Marie Siebke Trøseid, Unni Gopinathan, Peter Kierulf, Jens P Berg, Petter Brandtzaeg & Carola E Henriksson. (2011) LPS from Neisseria meningitidis is crucial for inducing monocyte- and microparticle-associated tissue factor activity but not for tissue factor expression . Innate Immunity 18:4, pages 580-591.
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Hans Christian D. Aass, Reidun Øvstebø, Anne-Marie S. Trøseid, Peter Kierulf, Jens Petter Berg & Carola Elisabeth Henriksson. (2011) Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry Part A 79A:12, pages 990-999.
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C.E. Henriksson, M. Hellum, K.B.F. Haug, H.C. Aass, G.B. Joø, R. Øvstebø, A.M. Trøseid, O. Klingenberg & P. Kierulf. (2011) Anticoagulant effects of an antidiabetic drug on monocytes in vitro. Thrombosis Research 128:5, pages e100-e106.
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Reidun Øvstebø, Ole Kristoffer Olstad, Berit Brusletto, Anne Sophie Møller, Audun Aase, Kari Bente Foss Haug, Petter Brandtzaeg & Peter Kierulf. (2008) Identification of Genes Particularly Sensitive to Lipopolysaccharide (LPS) in Human Monocytes Induced by Wild-Type versus LPS-Deficient Neisseria meningitidis Strains . Infection and Immunity 76:6, pages 2685-2695.
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Angela Best, Giovi Hidalgo, Katherine Mitchell & John R. Yannelli. (2007) Issues concerning the large scale cryopreservation of peripheral blood mononuclear cells (PBMC) for immunotherapy trials. Cryobiology 54:3, pages 294-297.
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Sjur Reppe, Lis Stilgren, Bo Abrahamsen, Ole K. Olstad, Fadila Cero, Kim Brixen, Lise Sofie Nissen-Meyer & Kaare M. Gautvik. (2007) Abnormal muscle and hematopoietic gene expression may be important for clinical morbidity in primary hyperparathyroidism. American Journal of Physiology-Endocrinology and Metabolism 292:5, pages E1465-E1473.
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C.E. Henriksson, O. Klingenberg, M. Hellum, K.S. Landsverk, G.B. Joø, Å.B. Westvik & P. Kierulf. (2007) Calcium ionophore-induced de-encryption of tissue factor in monocytes is associated with extensive cell death. Thrombosis Research 119:5, pages 621-630.
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Christine Krueger, Jonathan P. Schneck & Mathias Oelke. (2004) Quality and quantity: new strategies to improve immunotherapy of cancer. Trends in Molecular Medicine 10:5, pages 205-208.
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J Seager Danciger, M Lutz, S Hama, D Cruz, A Castrillo, J Lazaro, R Phillips, B Premack & J Berliner. (2004) Method for large scale isolation, culture and cryopreservation of human monocytes suitable for chemotaxis, cellular adhesion assays, macrophage and dendritic cell differentiation. Journal of Immunological Methods 288:1-2, pages 123-134.
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Anne-Sophie W. MøllerReidun Øvstebø, Åse-Brit Westvik, Gun Britt Joø, Kari-Bente F. Haug & Peter Kierulf. (2016) Effects of bacterial cell wall components (PAMPs) on the expression of monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α) and the chemokine receptor CCR2 by purified human blood monocytes. Journal of Endotoxin Research 9:6, pages 349-360.
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Reidun Øvstebø, Kari Bente Foss Haug, Knut Lande & Peter Kierulf. (2003) PCR-based Calibration Curves for Studies of Quantitative Gene Expression in Human Monocytes: Development and Evaluation. Clinical Chemistry 49:3, pages 425-432.
Crossref
Per Kr Lund, Ellen Namork, Sverre Henning Brorson, Åse-Brit Westvik, Gun Britt Joø, Reidun Øvstebø & Peter Kierulf. (2002) The fate of monocytes during 24 h of culture as revealed by flow cytometry and electron microscopy. Journal of Immunological Methods 270:1, pages 63-76.
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