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Diatom Research Volume 26, 2011 - Issue 1
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Original Articles

A protocol for a single-cell PCR of diatoms from fixed samples: method validation using Ditylum brightwellii (T. West) Grunow

Imke Lang Cyano Biofuels GmbH, Berlin, Germany; Department of Biology, Mount Allison University, Sackville, CanadaCorrespondence[email protected]
&
Irena Kaczmarska Department of Biology, Mount Allison University, Sackville, Canada
Pages 43-49 | Received 08 Jun 2009, Accepted 26 Aug 2010, Published online: 08 Jun 2011

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Adrian Kryk, MaŁgorzata BA˛k, Ewa GÓrecka, Catherine Riaux-Gobin, John Bemiasa, Etienne Bemanaja, Chunlian Li, PrzemysŁaw DA˛Bek & Andrzej Witkowski. (2020) Marine diatom assemblages of the Nosy Be Island coasts, NW Madagascar: species composition and biodiversity using molecular and morphological taxonomy. Systematics and Biodiversity 18:2, pages 161-180.
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Oliver Skibbe, Jonas Zimmermann, Wolf-Henning Kusber, Nélida Abarca, Krisztina Buczkó & Regine Jahn. (2018) Gomphoneis tegelensis sp. nov. (Bacillariophyceae): a morphological and molecular investigation based on selected single cells. Diatom Research 33:2, pages 251-262.
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Diba A. Khan-Bureau, Eduardo A. Morales, Luc Ector, Michael S. Beauchene & Louise A. Lewis. (2016) Characterization of a new species in the genus Didymosphenia and of Cymbella janischii (Bacillariophyta) from Connecticut, USA. European Journal of Phycology 51:2, pages 203-216.
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Articles from other publishers (21)

Andréa de Oliveira da Rocha Franco, Matt Peter Ashworth, Debbie Du Preez, Eileen Campbell, Rodrigo Maggioni, Schonna R. Manning & Clarisse Odebrecht. (2023) Surf-diatoms from sandy beaches on the Eastern Coast of South America (Brazil): the identification of Anaulus Ehrenberg and Asterionellopsis Round . Botanica Marina 66:5, pages 405-421.
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Minseok Jeong, Yitong Wang, Jong Im Kim & Woongghi Shin. (2023) Multigene phylogeny reveals a cryptic diversity in the genus Dinobryon (Chrysophyceae) with integrative description of five new species. Frontiers in Plant Science 14.
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Mónika Duleba, Angéla Földi, Adrienn Micsinai, Gábor Várbíró, Anita Mohr, Rita Sipos, Gyula Szabó, Krisztina Buczkó, Zsuzsa Trábert, Keve T. Kiss, Tibor Bíró, Edit Vadkerti & Éva Ács. (2021) Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats. Ecological Indicators 130, pages 108105.
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Daniel I. Wolf & Morgan L. Vis. (2019) Stream Algal Biofilm Community Diversity Along An Acid Mine Drainage Recovery Gradient Using Multimarker Metabarcoding. Journal of Phycology 56:1, pages 11-22.
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A. Goldenberg-Vilar, R. Álvarez-Troncoso, V. Roldán & Saúl Blanco. 2020. Modern Trends in Diatom Identification. Modern Trends in Diatom Identification 221 237 .
Demetrio Mora, Nélida Abarca, Sebastian Proft, José H. Grau, Neela Enke, Javier Carmona, Oliver Skibbe, Regine Jahn & Jonas Zimmermann. (2019) Morphology and metabarcoding: a test with stream diatoms from Mexico highlights the complementarity of identification methods. Freshwater Science 38:3, pages 448-464.
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Brajogopal Samanta, James M. Ehrman & Irena Kaczmarska. (2018) A consensus secondary structure of ITS2 for the diatom Order Cymatosirales (Mediophyceae, Bacillariophyta) and reappraisal of the order based on DNA, morphology, and reproduction. Molecular Phylogenetics and Evolution 129, pages 117-129.
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N. V. Annenkova. (2018) Identification of Lake Baikal Plankton Dinoflagellates from the Genera Gyrodinium and Gymnodinium Using Single-Cell PCR. Russian Journal of Genetics 54:11, pages 1302-1313.
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A. Hernández-Rosas, M. E. Meave del Castillo, J. Díaz-Larrea & F. Rodríguez. (2017) Single-cell PCR amplification of thecate dinoflagellates: a case study of Tripos (Dinophyceae). Journal of Applied Phycology 30:2, pages 1117-1124.
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Irena Kaczmarska, Benjamin S. Gray, James M. Ehrman & Mary Thaler. (2017) Sexual reproduction in plagiogrammacean diatoms: First insights into the early pennates. PLOS ONE 12:8, pages e0181413.
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Sung Min An, Dong Han Choi, Jung Ho Lee, Howon Lee & Jae Hoon Noh. (2017) Identification of benthic diatoms isolated from the eastern tidal flats of the Yellow Sea: Comparison between morphological and molecular approaches. PLOS ONE 12:6, pages e0179422.
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Keely E. Lefebvre, Paul B. Hamilton & Frances R. Pick. (2017) A comparison of molecular markers and morphology for Neidium taxa (Bacillariophyta) from eastern North America . Journal of Phycology 53:3, pages 680-702.
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Eveline Pinseel, Bart Van de Vijver, Jan Kavan, Elie Verleyen & Kateřina Kopalová. (2016) Diversity, ecology and community structure of the freshwater littoral diatom flora from Petuniabukta (Spitsbergen). Polar Biology 40:3, pages 533-551.
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Andréa de Oliveira da Rocha Franco, Ng Haig They, Luis Gustavo de Castro Canani, Rodrigo Maggioni & Clarisse Odebrecht. (2016) Asterionellopsis tropicalis (Bacillariophyceae): a new tropical species found in diatom accumulations . Journal of Phycology 52:5, pages 888-895.
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Ian A. Luddington, Connie Lovejoy & Irena Kaczmarska. (2016) Species-rich meta-communities of the diatom order Thalassiosirales in the Arctic and northern Atlantic Ocean. Journal of Plankton Research 38:4, pages 781-797.
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Paul B. Hamilton, Keely E. Lefebvre & Roger D. Bull. (2015) Single cell PCR amplification of diatoms using fresh and preserved samples. Frontiers in Microbiology 6.
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Joana Amorim Visco, Laure Apothéloz-Perret-Gentil, Arielle Cordonier, Philippe Esling, Loïc Pillet & Jan Pawlowski. (2015) Environmental Monitoring: Inferring the Diatom Index from Next-Generation Sequencing Data. Environmental Science & Technology 49:13, pages 7597-7605.
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Irena Kaczmarska, Laura Mather, Ian A. Luddington, France Muise & James M. Ehrman. (2014) Cryptic diversity in a cosmopolitan diatom known as Asterionellopsis glacialis (Fragilariaceae): Implications for ecology, biogeography, and taxonomy . American Journal of Botany 101:2, pages 267-286.
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X.G. Chen, J. Zhang, Y. Huang & Y.P. Hou. (2013) Diatom taxa identification based on single-cell isolation and rDNA sequencing. Forensic Science International: Genetics Supplement Series 4:1, pages e308-e309.
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Michael L. MacGillivaryIrena Kaczmarska. (2012) Genetic differentiation within the Paralia longispina (Bacillariophyta) species complex . Botany 90:3, pages 205-222.
Crossref
Michael L. MacGillivary & Irena Kaczmarska. (2011) Survey of the Efficacy of a Short Fragment of the rbc L Gene as a Supplemental DNA Barcode for Diatoms . Journal of Eukaryotic Microbiology 58:6, pages 529-536.
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