Advanced search
Publication Cover
Connective Tissue Research Volume 61, 2020 - Issue 3-4: Ex Vivo Models of Musculoskeletal Tissues
Submit an article Journal homepage
873
Views
23
CrossRef citations to date
0
Altmetric
Reviews

Intervertebral disc organ culture for the investigation of disc pathology and regeneration – benefits, limitations, and future directions of bioreactors

Judith-Johanna PfannkucheAO Research Institute Davos, Davos, Switzerland;Department of Orthopedic and Trauma Surgery, University Medical Center Freiburg, Albert-Ludwigs University of Freiburg, Freiburg, GermanyORCID Iconhttps://orcid.org/0000-0003-2302-7671View further author information
ORCID Icon,
Wei GuoAO Research Institute Davos, Davos, Switzerland;The first affiliated hospital of Sun Yat-sen University, Guangzhou, ChinaORCID Iconhttps://orcid.org/0000-0002-0218-9500View further author information
ORCID Icon,
Shangbin CuiAO Research Institute Davos, Davos, Switzerland;The first affiliated hospital of Sun Yat-sen University, Guangzhou, ChinaORCID Iconhttps://orcid.org/0000-0002-2069-7891View further author information
ORCID Icon,
Junxuan MaAO Research Institute Davos, Davos, SwitzerlandORCID Iconhttps://orcid.org/0000-0001-8923-9832View further author information
ORCID Icon,
Gernot LangDepartment of Orthopedic and Trauma Surgery, University Medical Center Freiburg, Albert-Ludwigs University of Freiburg, Freiburg, GermanyORCID Iconhttps://orcid.org/0000-0003-0685-1909View further author information
ORCID Icon,
Marianna PeroglioAO Research Institute Davos, Davos, SwitzerlandORCID Iconhttps://orcid.org/0000-0002-5458-3446View further author information
ORCID Icon,
R. Geoff RichardsAO Research Institute Davos, Davos, Switzerland;Department of Orthopedic and Trauma Surgery, University Medical Center Freiburg, Albert-Ludwigs University of Freiburg, Freiburg, GermanyORCID Iconhttps://orcid.org/0000-0002-7778-2480View further author information
ORCID Icon,
Mauro AliniAO Research Institute Davos, Davos, SwitzerlandORCID Iconhttps://orcid.org/0000-0002-0262-1412View further author information
ORCID Icon,
Sibylle GradAO Research Institute Davos, Davos, SwitzerlandORCID Iconhttps://orcid.org/0000-0001-9552-3653View further author information
ORCID Icon &
Zhen LiAO Research Institute Davos, Davos, SwitzerlandCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-9754-6389View further author information
ORCID Icon show all
Pages 304-321 | Received 30 Jul 2019, Accepted 04 Sep 2019, Published online: 26 Sep 2019

Citations (23)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (1)

Spencer E. Szczesny. (2020) Ex vivo models of musculoskeletal tissues. Connective Tissue Research 61:3-4, pages 245-247.
Read now

Articles from other publishers (22)

Hosni Cherif, Li Li, Joseph Snuggs, Xuan Li, Christopher Sammon, Jianyu Li, Lorne Beckman, Lisbet Haglund & Christine. L． Le Maitre. (2024) Injectable hydrogel induces regeneration of naturally degenerate human intervertebral discs in a loaded organ culture model. Acta Biomaterialia 176, pages 201-220.
Crossref
Junxuan Ma, Janick Eglauf, Sibylle Grad, Mauro Alini & Tiziano Serra. (2023) Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth. Advanced Science 11:11.
Crossref
Zihan Wang, Xin Chen, Nan Chen, Hongjie Yan, Ke Wu, Jitao Li, Qingyuan Ru, Rongrong Deng, Xin Liu & Ran Kang. (2023) Mechanical Factors Regulate Annulus Fibrosus (AF) Injury Repair and Remodeling: A Review. ACS Biomaterials Science & Engineering 10:1, pages 219-233.
Crossref
Andrea Vernengo, Helen Bumann, Nadine Kluser, Astrid Soubrier, Amra Šećerović, Jan Gewiess, Jan Ulrich Jansen, Cornelia Neidlinger-Wilke, Hans-Joachim Wilke & Sibylle Grad. (2023) Chemonucleolysis combined with dynamic loading for inducing degeneration in bovine caudal intervertebral discs. Frontiers in Bioengineering and Biotechnology 11.
Crossref
Aapo Ristaniemi, Amra Šećerović, Vincent Dischl, Francesco Crivelli, Sarah Heub, Diane Ledroit, Gilles Weder, Sibylle Grad & Stephen J. Ferguson. (2023) Physiological and degenerative loading of bovine intervertebral disc in a bioreactor: A finite element study of complex motions. Journal of the Mechanical Behavior of Biomedical Materials 143, pages 105900.
Crossref
Elias Salzer, Vivian H. M. Mouser, Jurgen A. Bulsink, Marianna A. Tryfonidou & Keita Ito. (2023) Dynamic loading leads to increased metabolic activity and spatial redistribution of viable cell density in nucleus pulposus tissue. JOR SPINE 6:1.
Crossref
Shirley N. Tang, Andres F. Bonilla, Nadeen O. Chahine, Aimee C. Colbath, Jeremiah T. Easley, Sibylle Grad, Lisbet Haglund, Christine L. Le Maitre, Victor Leung, Annette M. McCoy, Devina Purmessur, Simon Y. Tang, Stephan Zeiter & Lachlan J. Smith. (2022) Controversies in spine research: Organ culture versus in vivo models for studies of the intervertebral disc. JOR SPINE 5:4.
Crossref
Xiao‐Chuan Li, Shao‐Jian Luo, Wu Fan, Tian‐Li Zhou, Chun‐Ming Huang & Mao‐Sheng Wang. (2022) M2 macrophage‐conditioned medium inhibits intervertebral disc degeneration in a tumor necrosis factor‐α‐rich environment. Journal of Orthopaedic Research 40:11, pages 2488-2501.
Crossref
Amra Šećerović, Aapo Ristaniemi, Shangbin Cui, Zhen Li, Astrid Soubrier, Mauro Alini, Stephen J Ferguson, Gilles Weder, Sarah Heub, Diane Ledroit & Sibylle Grad. (2022) Toward the Next Generation of Spine Bioreactors: Validation of an Ex Vivo Intervertebral Disc Organ Model and Customized Specimen Holder for Multiaxial Loading. ACS Biomaterials Science & Engineering 8:9, pages 3969-3976.
Crossref
Shangbin Cui, Wenyue Li, Graciosa Q. Teixeira, Cornelia Neidlinger‐Wilke, Hans‐Joachim Wilke, Lisbet Haglund, Hongwei Ouyang, R. Geoff Richards, Sibylle Grad, Mauro Alini & Zhen Li. (2022) Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration. JOR SPINE 5:3.
Crossref
Elias Salzer, Vivian H. M. Mouser, Marianna A. Tryfonidou & Keita Ito. (2021) A bovine nucleus pulposus explant culture model. Journal of Orthopaedic Research 40:9, pages 2089-2102.
Crossref
Paola Bermudez-Lekerika, Katherine B. Crump, Sofia Tseranidou, Andrea Nüesch, Exarchos Kanelis, Ahmad Alminnawi, Laura Baumgartner, Estefano Muñoz-Moya, Roger Compte, Francesco Gualdi, Leonidas G. Alexopoulos, Liesbet Geris, Karin Wuertz-Kozak, Christine L. Le Maitre, Jérôme Noailly & Benjamin Gantenbein. (2022) Immuno-Modulatory Effects of Intervertebral Disc Cells. Frontiers in Cell and Developmental Biology 10.
Crossref
Andrea Mainardi, Elena Cambria, Paola Occhetta, Ivan Martin, Andrea Barbero, Stefan Schären, Arne Mehrkens & Olga Krupkova. (2022) Intervertebral Disc-on-a-Chip as Advanced In Vitro Model for Mechanobiology Research and Drug Testing: A Review and Perspective. Frontiers in Bioengineering and Biotechnology 9.
Crossref
Shuo Zhang, Peng Wang, Binwu Hu, Weijian Liu, Xiao Lv, Songfeng Chen & Zengwu Shao. (2022) HSP90 Inhibitor 17-AAG Attenuates Nucleus Pulposus Inflammation and Catabolism Induced by M1-Polarized Macrophages. Frontiers in Cell and Developmental Biology 9.
Crossref
Sweety Evangeli Malli, Pranav Kumbhkarn, Ankush Dewle & Akshay Srivastava. (2021) Evaluation of Tissue Engineering Approaches for Intervertebral Disc Regeneration in Relevant Animal Models. ACS Applied Bio Materials 4:11, pages 7721-7737.
Crossref
Penghui Zhang, Xizhe Liu, Peng Guo, Xianlong Li, Zhongyuan He, Zhen Li, Martin J. Stoddart, Sibylle Grad, Wei Tian, Dafu Chen, Xuenong Zou, Zhiyu Zhou & Shaoyu Liu. (2021) Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration. Bioactive Materials 6:10, pages 3097-3108.
Crossref
Thomas Christiani, Karen Mys, Karl Dyer, Jennifer Kadlowec, Cristina Iftode & Andrea Jennifer Vernengo. (2021) Using embedded alginate microparticles to tune the properties of in situ forming poly( N ‐isopropylacrylamide)‐graft‐chondroitin sulfate bioadhesive hydrogels for replacement and repair of the nucleus pulposus of the intervertebral disc . JOR SPINE 4:3.
Crossref
Shangbin Cui, Zhiyu Zhou, Xu Chen, Fuxin Wei, R. Geoff Richards, Mauro Alini, Sibylle Grad & Zhen Li. (2021) Transcriptional profiling of intervertebral disc in a post‐traumatic early degeneration organ culture model. JOR SPINE 4:3.
Crossref
Minhao Zhou, Shiyin Lim & Grace D. O’Connell. (2021) A Robust Multiscale and Multiphasic Structure-Based Modeling Framework for the Intervertebral Disc. Frontiers in Bioengineering and Biotechnology 9.
Crossref
Wuwei Ren, Shangbin Cui, Mauro Alini, Sibylle Grad, Quanyu Zhou, Zhen Li & Daniel Razansky. (2021) Noninvasive multimodal fluorescence and magnetic resonance imaging of whole-organ intervertebral discs. Biomedical Optics Express 12:6, pages 3214.
Crossref
Fabrizio Russo, Luca Ambrosio, Marianna Peroglio, Wei Guo, Sebastian Wangler, Jan Gewiess, Sibylle Grad, Mauro Alini, Rocco Papalia, Gianluca Vadalà & Vincenzo Denaro. (2021) A Hyaluronan and Platelet-Rich Plasma Hydrogel for Mesenchymal Stem Cell Delivery in the Intervertebral Disc: An Organ Culture Study. International Journal of Molecular Sciences 22:6, pages 2963.
Crossref
Benjamin Gantenbein, Andreas S. Croft & Marie Larraillet. 2020. Fluorescence Methods for Investigation of Living Cells and Microorganisms. Fluorescence Methods for Investigation of Living Cells and Microorganisms.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.