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Original Articles

Immobilized Metal Ion Affinity Chromatography (IMAC) Chemistry and Bioseparation Applications

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Pages 49-106 | Published online: 24 Sep 2006

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (8)

Hüseyin Alkan, Şeyda Ceylan Cömert, Fatma Gürbüz, Mehmet Doğru & Mehmet Odabaşı. (2017) Cu2+-attached pumice particles embedded composite cryogels for protein purification. Artificial Cells, Nanomedicine, and Biotechnology 45:1, pages 90-97.
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Birnur Akkaya & Recep Akkaya. (2012) Cross-Linked Bentonite-Acrylamide-histidine-Based Metal-Chelate Affinity Microcomposites for Lysozyme Separation From Egg White. Separation Science and Technology 48:2, pages 272-280.
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Rong Li, Yan Wang, Guo Liang Chen, Mei Shi, Xiao Gang Wang, Bin Chen & Jian Bin Zheng. (2010) A Novel Silica-Based Metal Chelate Stationary Phase—L-Glutamic Acid–Copper(II). Separation Science and Technology 46:2, pages 309-314.
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R. Gutiérrez, E. M. Martín del Valle & M. A. Galán. (2007) Immobilized Metal‐Ion Affinity Chromatography: Status and Trends. Separation & Purification Reviews 36:1, pages 71-111.
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Sadhana Sharma & Gopal P. Agarwal. (2002) COMPARATIVE STUDIES ON THE METAL SORPTION CHARACTERISTICS OF CHELATING GELS FOR IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY. Separation Science and Technology 37:15, pages 3491-3511.
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LeonardD. Holmes & MartinR. Schiller. (1997) Immobilized Iron(III) Metal Affinity Chromatography for the Separation of Phosphorylated Macromolecules: Ligands and Applications. Journal of Liquid Chromatography & Related Technologies 20:1, pages 123-142.
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JaimeE. Ramírez-Vick & AntonioA. García. (1996) Recent Developments in the Use of Group-Specific Ligands for Affinity Bioseparations. Separation and Purification Methods 25:2, pages 85-129.
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Articles from other publishers (108)

Omayma Abdelrazak Amin, Amira Fawzy El-Yazbi, Doha Mohamed Elmoghny & Rania Bakry. (2022) Immobilized metal affinity-silica based support for the solid phase extraction of antimicrobials from water. Microchemical Journal 183, pages 107968.
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Rachel Irankunda, Jairo Andrés Camaño Echavarría, Cédric Paris, Loïc Stefan, Stéphane Desobry, Katalin Selmeczi, Laurence Muhr & Laetitia Canabady-Rochelle. (2022) Metal-Chelating Peptides Separation Using Immobilized Metal Ion Affinity Chromatography: Experimental Methodology and Simulation. Separations 9:11, pages 370.
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Raquel Bromberg, Kai Cai, Yirui Guo, Daniel Plymire, Tabitha Emde, Maciej Puzio, Dominika Borek & Zbyszek Otwinowski. (2022) The His-tag as a decoy modulating preferred orientation in cryoEM. Frontiers in Molecular Biosciences 9.
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Alexandra Rak, Svetlana Donina, Yana Zabrodskaya, Larisa Rudenko & Irina Isakova-Sivak. (2022) Cross-Reactivity of SARS-CoV-2 Nucleocapsid-Binding Antibodies and Its Implication for COVID-19 Serology Tests. Viruses 14:9, pages 2041.
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Juan M. Bolivar, John M. Woodley & Roberto Fernandez-Lafuente. (2022) Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization. Chemical Society Reviews 51:15, pages 6251-6290.
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Yaling Li, Hao Ai, Liangzhi Qiao, Yinghong Wang & Kaifeng Du. (2022) Fabrication and characterization of hierarchical porous Ni2+ doped hydroxyapatite microspheres and their enhanced protein adsorption capacity. Chinese Journal of Chemical Engineering 45, pages 238-247.
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İhsan Alacabey, Ömür Acet, Burcu Önal, Emrah Dikici, Veyis Karakoç, Fatma Gürbüz, Hüseyin Alkan & Mehmet Odabaşı. (2020) Pumice particle interface: a case study for immunoglobulin G purification. Polymer Bulletin 78:10, pages 5593-5607.
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Sarah El Hajj, Tatiana Sepulveda-Rincon, Cédric Paris, Tristan Giraud, Gizella Csire, Loic Stefan, Katalin Selmeczi, Jean-Michel Girardet, Stéphane Desobry, Said Bouhallab, Laurence Muhr, Caroline Gaucher & Laetitia Canabady-Rochelle. 2021. Biologically Active Peptides. Biologically Active Peptides 455 494 .
Meijia Huang, Xin Li, Ping Tong, Jinyan Gao, Juanli Yuan, Anshu Yang, Hongbing Chen & Yong Wu. (2020) Potential allergenicity assessment after bovine apo‐α‐lactalbumin binding to calcium ion. Journal of Food Biochemistry 44:9.
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Vlad-Stefan Raducanu, Ioannis Isaioglou, Daniela-Violeta Raducanu, Jasmeen S. Merzaban & Samir M. Hamdan. (2020) Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair. Journal of Biological Chemistry 295:34, pages 12214-12223.
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Igor Tadeu Lazzarotto Bresolin, Iara Rocha Antunes Pereira Bresolin & Sônia Maria Alves Bueno. (2019) Evaluation of Iminodiacetic Acid (IDA) as an Ionogenic Group for Adsorption of IgG1 Monoclonal Antibodies by Membrane Chromatography. Applied Biochemistry and Biotechnology 191:2, pages 810-823.
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Laurence Muhr, Steve Pontvianne, Katalin Selmeczi, Cédric Paris, Sandrine Boschi‐Muller & Laetitia Canabady‐Rochelle. (2020) Chromatographic separation simulation of metal‐chelating peptides from surface plasmon resonance binding parameters. Journal of Separation Science 43:11, pages 2031-2041.
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Vishwanath Hebbi, Devendra Kumar & Anurag S. Rathore. (2020) Process intensification in peptide manufacturing: Recombinant lethal toxin neutralizing factor (rLTNF) as a case study. Process Biochemistry 90, pages 193-203.
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Evangelia G. Chronopoulou, Christina Varotsou, Nikolaos Georgakis, Georgios Premetis, Elisavet Ioannou & Nikolaos E. Labrou. 2020. Targeting Enzymes for Pharmaceutical Development. Targeting Enzymes for Pharmaceutical Development 235 243 .
Man Zhao, Junhui Rong, Juan Han, Yang Zhou, Chunmei Li, Lei Wang, Yanli Mao & Yun Wang. (2019) Novel Synthesis Strategy for Biocatalyst: Fast Purification and Immobilization of His- and ELP-Tagged Enzyme from Fermentation Broth. ACS Applied Materials & Interfaces 11:35, pages 31878-31888.
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Tacias-Pascacio, Ortiz, Rueda, Berenguer-Murcia, Acosta, Aranaz, Civera, Fernandez-Lafuente & Alcántara. (2019) Dextran Aldehyde in Biocatalysis: More Than a Mere Immobilization System. Catalysts 9:7, pages 622.
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Álvaro González-Garcinuño, Sonia Ruiz, Álvaro Sánchez-Muñoz, Antonio Tabernero & Eva Martín del Valle. (2019) Biotechnological strategies to produce levan: Mass transfer and techno-economical evaluation. Chemical Engineering and Processing - Process Intensification 141, pages 107529.
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Rong Zeng, Bu‐Kun Jin, Zhong‐Hua Yang, Rong Guan & Can Quan. (2018) Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His‐tagged protein. Journal of Applied Polymer Science 136:15.
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Christine F. Markwalter, Andrew G. Kantor, Carson P. Moore, Kelly A. Richardson & David W. Wright. (2018) Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics. Chemical Reviews 119:2, pages 1456-1518.
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Vishwanath Hebbi, Kathiresan Pandi, Devendra Kumar, Claire Komives & Anurag S Rathore. (2018) Process for production and purification of lethal toxin neutralizing factor (LTNF) from E. coli and its economic analysis . Journal of Chemical Technology & Biotechnology 93:4, pages 959-967.
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Bin Chen, Ning Zhang, Chen Li, Rong Li & An Fan. (2017) Synthesis and chromatographic characteristics of iminodisuccinic acid-functionalized silica stationary phase. Journal of Chromatography A 1530, pages 120-128.
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Aleicia Holland, Chris M. Wood, D. Scott Smith, Tiago Gabriel Correia & Adalberto L. Val. (2017) Nickel toxicity to cardinal tetra (Paracheirodon axelrodi) differs seasonally and among the black, white and clear river waters of the Amazon basin. Water Research 123, pages 21-29.
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Qianwei Li & Geoffrey Michael Gadd. (2017) Biosynthesis of copper carbonate nanoparticles by ureolytic fungi. Applied Microbiology and Biotechnology 101:19, pages 7397-7407.
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Dmitry A. Kurdyukov, Ekaterina N. Chernova, Yana V. Russkikh, Daniil A. Eurov, Vasily V. Sokolov, Anna A. Bykova, Vladimir V. Shilovskikh, Olga A. Keltsieva, Eugenii V. Ubyivovk, Yuri A. Anufrikov, Anna V. Fedorova, Artem A. Selyutin, Nicolay G. Sukhodolov, Ekaterina P. Podolskaya & Valery G. Golubev. (2017) Ni-functionalized submicron mesoporous silica particles as a sorbent for metal affinity chromatography. Journal of Chromatography A 1513, pages 140-148.
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Meng-Meng Wang, Qing Chen, Dan-Dan Zhang, Xu-Wei Chen & Ming-Li Chen. (2017) Tetra-nickel substituted polyoxotungsate as an efficient sorbent for the isolation of His6-tagged proteins from cell lysate. Talanta 171, pages 173-178.
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Ekaterina V. Shreyner, Marina L. Alexandrova, Nikolay G. Sukhodolov, Artem A. Selyutin & Ekaterina P. Podolskaya. (2017) Extraction of the insecticide dieldrin from water and biological samples by metal affinity chromatography. Mendeleev Communications 27:3, pages 304-306.
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M.S. Mohy Eldin, S. Abdel Rahman & G.F. El fawal. (2017) Novel immobilized Cu+2 ion grafted cellophane membranes for affinity separation of His-Tag Chitinase. Arabian Journal of Chemistry 10, pages S3652-S3663.
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Nuray Yılmaz Baran, Ömür Acet & Mehmet Odabaşı. (2017) Efficient adsorption of hemoglobin from aqueous solutions by hybrid monolithic cryogel column. Materials Science and Engineering: C 73, pages 15-20.
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Khadijeh Alishah, Sedigheh Asad, Khosro Khajeh & Neda Akbari. (2016) Utilizing intein-mediated protein cleaving for purification of uricase, a multimeric enzyme. Enzyme and Microbial Technology 93-94, pages 92-98.
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Jane T. Mooney, Dale P. Fredericks, Thorkild Christensen, Christine Bruun Schiødt & Milton T. W. Hearn. (2015) N-terminal processing of affinity-tagged recombinant proteins purified by IMAC procedures. Journal of Molecular Recognition 28:7, pages 401-412.
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Chunfang Zhang, Dale Fredericks, Darant Longford, Eva Campi, Tamsin Sawford & Milton T. W. Hearn. (2014) Changed loading conditions and lysate composition improve the purity of tagged recombinant proteins with tacn‐based IMAC adsorbents. Biotechnology Journal 10:3, pages 480-489.
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J. Gu & R. Codd. (2015) The resolution of two clinical agents, bleomycin and desferrioxamine B, from a Streptomyces verticillus fermentation mixture using multi-dimensional immobilised metal ion affinity chromatography. RSC Advances 5:5, pages 3443-3453.
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Ke-Xue Zhu, Xiao-Ping Wang & Xiao-Na Guo. (2015) Isolation and characterization of zinc-chelating peptides from wheat germ protein hydrolysates. Journal of Functional Foods 12, pages 23-32.
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Jane T. Mooney, Dale Fredericks, Thorkild Christensen & Milton T. W. Hearn. (2014) Removal of cleavage slow points from affinity tags used in the IMAC purification of recombinant proteins. Biotechnology Journal 9:8, pages 1023-1032.
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Xiaofeng Wang, Ligang Xing, Yang Shu, Xuwei Chen & Jianhua Wang. (2014) Novel polymeric ionic liquid microspheres with high exchange capacity for fast extraction of plasmid DNA. Analytica Chimica Acta 837, pages 64-69.
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Jane T. Mooney, Dale P. Fredericks, Chunfang Zhang, Thorkild Christensen, Christina Jespergaard, Christine Bruun Schiødt & Milton T.W. Hearn. (2014) Purification of a recombinant human growth hormone by an integrated IMAC procedure. Protein Expression and Purification 94, pages 85-94.
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Jane T. Mooney, Dale P. Fredericks & Milton T.W. Hearn. (2013) Application of an IMAC cassette for the purification of N-terminally tagged proteins. Separation and Purification Technology 120, pages 265-274.
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Beg?m Akduman, Murat Uygun, Deniz Akta? Uygun & Mari?n Antal?k. (2013) Fe3O4 magnetic core coated by silver and functionalized with N-acetyl cysteine as novel nanoparticles in ferritin adsorption. Journal of Nanoparticle Research 15:4.
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Chan Wang, Bo Li & Jing Ao. (2012) Separation and identification of zinc-chelating peptides from sesame protein hydrolysate using IMAC-Zn2+ and LC–MS/MS. Food Chemistry 134:2, pages 1231-1238.
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Aming Zhang, Cheng Zhang, Veena Warikoo, Jonathan Forstrom & Frank Riske. (2011) A modified IMAC method for the capture of target protein from mammalian cell culture harvest containing metal chelating species. Biotechnology and Bioengineering 109:3, pages 747-753.
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Ya‐Ching Lin, Shu‐Ting Lin, Cheng‐Yi Chen & Sheng‐Chi Wu. (2011) Enterovirus 71 adsorption on metal ion‐composite chitosan beads. Biotechnology Progress 28:1, pages 206-214.
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Nuri Ünlü, Şeyda Ceylan, Mahmut Erzengin & Mehmet Odabaşı. (2011) Investigation of protein adsorption performance of Ni 2+ ‐attached diatomite particles embedded in composite monolithic cryogels . Journal of Separation Science 34:16-17, pages 2173-2180.
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Rong Li, Yan Wang, Guo‐Liang Chen, Mei‐Shi, Xiao‐Gang Wang, Jian‐Bin Zheng & Bin Chen. (2011) Effects of Immobilized Metal Ion on Retention Behaviors of Proteins in Metal Chelate Affinity Chromatography. Journal of the Chinese Chemical Society 58:2, pages 215-221.
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Lidiana Cristina de Góes, Everson Alves Miranda & Sonia Maria Alves Bueno. (2010) Interaction of histidine-tagged human proinsulin with immobilized nickel ion: Effect of chelating ligand and thermodynamics analysis. Colloids and Surfaces A: Physicochemical and Engineering Aspects 369:1-3, pages 176-185.
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