Advanced search
Publication Cover
Critical Reviews in Biotechnology Volume 39, 2019 - Issue 2
Submit an article Journal homepage
42,876
Views
58
CrossRef citations to date
0
Altmetric
Review Article

Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production

Wan-Cang LiuState Key Laboratory of Bioactive Substance and Function of Natural Medicines; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China; ;Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, U.S.A; ;Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Medicinal Biotechnology, Beijing, P. R. China; View further author information
,
Sarah InwoodBiotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, U.S.A; ;Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USAView further author information
,
Ting GongState Key Laboratory of Bioactive Substance and Function of Natural Medicines; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China; View further author information
,
Ashish SharmaBiotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, U.S.A; View further author information
,
Li-Yan YuChinese Academy of Medical Sciences & Peking Union Medical College, Institute of Medicinal Biotechnology, Beijing, P. R. China; Correspondence[email protected] [email protected]
View further author information
&
Ping ZhuState Key Laboratory of Bioactive Substance and Function of Natural Medicines; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China; Correspondence[email protected] [email protected]
View further author information
Pages 258-271 | Received 22 May 2018, Accepted 21 Oct 2018, Published online: 02 Jan 2019

Citations (58)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (2)

Mohammad Mahmoodi & Ehsan Nassireslami. (2022) Control algorithms and strategies of feeding for fed-batch fermentation of Escherichia coli: a review of 40 years of experience. Preparative Biochemistry & Biotechnology 52:7, pages 823-834.
Read now
Lin Cui, Huoqing Huang, Honglian Zhang, Xiaolu Wang, Xing Qin, Tao Tu, Jie Zhang, Xiaoyun Su, Huimin Yu, Yingguo Bai, Huiying Luo, Bin Yao & Yuan Wang. (2022) Recombinant expression of hen egg white lysozyme with the assistance of xylanase fusion partner in Pichia pastoris. Bioengineered 13:5, pages 13860-13871.
Read now

Articles from other publishers (56)

Satya Sai Pavan Allampalli, Sandhya Sekhar & Senthilkumar Sivaprakasam. (2024) Enhanced production of human interferon α2b in glycoengineered Pichia pastoris by robust control of methanol feeding and implications of various control strategies. Biochemical Engineering Journal 201, pages 109152.
Crossref
Azadeh Eskandari, Nima Ghahremani Nezhad, Thean Chor Leow, Mohd Basyaruddin Abdul Rahman & Siti Nurbaya Oslan. (2023) Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system. World Journal of Microbiology and Biotechnology 40:1.
Crossref
Nils Wagner, Linxuan Wen, Cláudio J.R. Frazão & Thomas Walther. (2023) Next-generation feedstocks methanol and ethylene glycol and their potential in industrial biotechnology. Biotechnology Advances 69, pages 108276.
Crossref
Mengtong Jiang, Yuxin Liu, Hongjian Xue, Yiqi Wang, Conggang Wang, Fan Yang & Xianzhen Li. (2023) Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33. Protein Expression and Purification 208-209, pages 106277.
Crossref
Mohammad Amin Boojari, Fatemeh Rajabi Ghaledari, Ehsan Motamedian, Mehdi Soleimani & Seyed Abbas Shojaosadati. (2023) Developing a metabolic model‐based fed‐batch feeding strategy for Pichia pastoris fermentation through fine‐tuning of the methanol utilization pathway . Microbial Biotechnology 16:6, pages 1344-1359.
Crossref
Vivien Jessica Klein, Luciana Fernandes Brito, Fernando Perez‐Garcia, Trygve Brautaset & Marta Irla. (2023) Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol . Microbial Biotechnology 16:5, pages 1011-1026.
Crossref
Chao Liu, Jin-Song Gong, Chang Su, Heng Li, Jiufu Qin, Zheng-Hong Xu & Jin-Song Shi. (2023) Increasing gene dosage and chaperones co-expression facilitate the efficient dextranase expression in Pichia pastoris. LWT 181, pages 114753.
Crossref
Jewel Ann Joseph, Simen Akkermans, Elke Cornillie, Jerome Deberlanger & Jan F.M. Van Impe. (2023) Optimal culture medium selection and supplementation for recombinant thaumatin II production by Komagataella phaffii. Food and Bioproducts Processing 139, pages 190-203.
Crossref
Zheng Zhang, Penghui He, Shiying Hu, Yanqing Yu, Xiaoting Wang, Ali Raza Ishaq & Shouwen Chen. (2023) Promoting cell growth for bio-chemicals production via boosting the synthesis of L/D-alanine and D-alanyl-D-alanine in Bacillus licheniformis. World Journal of Microbiology and Biotechnology 39:5.
Crossref
Yanjie Jin, Na Yang, Da Teng, Ya Hao, Ruoyu Mao & Jianhua Wang. (2023) Molecular Modification of Kex2 P1’ Site Enhances Expression and Druggability of Fungal Defensin. Antibiotics 12:4, pages 786.
Crossref
Yangyang Li, Siqi Xie, Minhua Chen, Hao Li, Yehai Wang, Yan Fan, Kang An, Yu Wu & Weihua Xiao. (2023) Development of an antibody-ligand fusion protein scFvCD16A-sc4-1BBL in Komagataella phaffii with stimulatory activity for Natural Killer cells. Microbial Cell Factories 22:1.
Crossref
Xian Li, Beilei Shi, Jian-Wen Huang, Ziyin Zeng, Yu Yang, Lilan Zhang, Jian Min, Chun-Chi Chen & Rey-Ting Guo. (2023) Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris. Bioresources and Bioprocessing 10:1.
Crossref
Anamika Singh & Atul Narang. (2023) PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source. Frontiers in Bioengineering and Biotechnology 11.
Crossref
Sirirat Changming, Prachumporn Kongsaree, Imrana Niaz Sultan, Afrasiab Khan Tareen, Wirat Vanichsriratana, Sarote Sirisansaneeyakul & Pramuk Parakulsuksatid. (2022) β-glucosidase production by recombinant Pichia pastoris strain Y1433 under optimal feed profiles of fed-batch cultivation. Folia Microbiologica 68:2, pages 245-256.
Crossref
Tatiana Ianshina, Anton Sidorin, Kristina Petrova, Maria Shubert, Anastasiya Makeeva, Elena Sambuk, Anastasiya Govdi, Andrey Rumyantsev & Marina Padkina. (2023) Effect of Methionine on Gene Expression in Komagataella phaffii Cells. Microorganisms 11:4, pages 877.
Crossref
Chatchol Kongsinkaew, Supenya Chittapun, Chanitchote Piyapittayanun, Viroj Boonyaratanakornkit, Sarintip Sooksai, Kittisak Ajariyakhajorn, Soisuda Pornpukdeewattana, Warawut Krusong, Tunyaboon Laemthong & Theppanya Charoenrat. (2023) Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate. Fermentation 9:3, pages 277.
Crossref
John H. Dupuis, Lennie K.Y. Cheung, Lenore Newman, Derek R. Dee & Rickey Y. Yada. (2022) Precision cellular agriculture: The future role of recombinantly expressed protein as food. Comprehensive Reviews in Food Science and Food Safety 22:2, pages 882-912.
Crossref
Jorge Barroca-Ferreira, Claudio J. Maia & Luís A. Passarinha. 2023. Advanced Methods in Structural Biology. Advanced Methods in Structural Biology 35 54 .
Gang Luo, Zijian Geng, Buayisham Kuerban, Yingqing Xu, Jingjing Yang, Jiying Liu & Muwang Li. (2022) Enhancement of HSA-pFSHβ production by disrupting YPS1 and supplementing N-acetyl-L-cysteine in Pichia pastoris. Frontiers in Microbiology 13.
Crossref
Anna Jéssica Duarte Silva, Crislaine Kelly da Silva Rocha & Antonio Carlos de Freitas. (2022) Standardization and Key Aspects of the Development of Whole Yeast Cell Vaccines. Pharmaceutics 14:12, pages 2792.
Crossref
Rongkang Hu, Ruiguo Cui, Qingqing Xu, Dongming Lan & Yonghua Wang. (2022) Controlling Specific Growth Rate for Recombinant Protein Production by Pichia pastoris Under Oxidation Stress in Fed-batch Fermentation. Applied Biochemistry and Biotechnology 194:12, pages 6179-6193.
Crossref
Clara Vida Galrão Corrêa Carneiro, Luana Assis Serra, Thályta Fraga Pacheco, Letícia Maria Mallmann Ferreira, Lívia Teixeira Duarte Brandão, Mariana Nogueira de Moura Freitas, Débora Trichez & João Ricardo Moreira de Almeida. (2022) Advances in Komagataella phaffii Engineering for the Production of Renewable Chemicals and Proteins. Fermentation 8:11, pages 575.
Crossref
Luqiang Jia, Teng Li, Zhenquan Yang, Tianyi Li & Jian Ding. (2022) Enhancing monellin production in Pichia pastoris fed-batch fermentation through an efficient on-line methanol/sorbitol co-feeding strategy. Journal of Cleaner Production 369, pages 133350.
Crossref
Vikas Kumar Dagar, Babbal, Shilpa Mohanty & Yogender Pal Khasa. (2022) Effect of N-glycosylation on secretion, stability, and biological activity of recombinant human interleukin-3 (hIL-3) in Pichia pastoris. 3 Biotech 12:9.
Crossref
Emils Bolmanis, Oskars Grigs, Andris Kazaks & Vytautas Galvanauskas. (2022) High-level production of recombinant HBcAg virus-like particles in a mathematically modelled P. pastoris GS115 Mut+ bioreactor process under controlled residual methanol concentration. Bioprocess and Biosystems Engineering 45:9, pages 1447-1463.
Crossref
Aibo Feng, Jingyao Zhou, Hongli Mao, Hualan Zhou & Jianguo Zhang. (2022) Heterologous protein expression enhancement of Komagataella phaffii by ammonium formate induction based on transcriptomic analysis. Biochemical Engineering Journal 185, pages 108503.
Crossref
Cristina Bustos, Johan Quezada, Rhonda Veas, Claudia Altamirano, Stephanie Braun-Galleani, Patrick Fickers & Julio Berrios. (2022) Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production. Metabolites 12:4, pages 346.
Crossref
Yuanke Guo, Yang Liao, Jing Wang, Chen Ma, Jialun Qin, Jiao Feng, Yan Li, Xin Wang & Kequan Chen. (2022) Methylotrophy of Pichia pastoris : Current Advances, Applications, and Future Perspectives for Methanol-Based Biomanufacturing . ACS Sustainable Chemistry & Engineering 10:5, pages 1741-1752.
Crossref
Robert E. Speight, Laura Navone, Leigh K. Gebbie, Jo-Anne L. Blinco & Wayne L. Bryden. (2022) Platforms to accelerate biomanufacturing of enzyme and probiotic animal feed supplements: discovery considerations and manufacturing implications. Animal Production Science 62:12, pages 1113-1128.
Crossref
Inês Farinha, Sílvia Baptista, Maria A. M. Reis & Filomena Freitas. (2022) Influence of Dissolved Oxygen Level on Chitin–Glucan Complex and Mannans Production by the Yeast Pichia pastoris. Life 12:2, pages 161.
Crossref
Hua ZHAO, Zhe ZHENG, Man ZHANG, Yihui WANG, Min ZHANG & Zhennai YANG. (2022) Fermentation optimization of rennet-producing Bacillus amyloliquefaciens GSBa-1 for high-density culture and its kinetic model. Food Science and Technology 42.
Crossref
Huan Xu, Mingyang Shi, Changsheng Shao, Hao Li, Jing Wu, Yin Yu, Fang Fang, Yugang Guo & Weihua Xiao. (2021) Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives. Microbial Cell Factories 20:1.
Crossref
Minjie Gao, Feiyang Duan, Liping Liu, Xiuyu Hu, Li Zhu, Yun Jiang & Xiaobei Zhan. (2021) An innovative strategy of recycling miscellaneous waste carbohydrates from high-fructose syrup production for Pichia pastoris fermentation. Journal of Cleaner Production 326, pages 129404.
Crossref
Francisco Ibáñez, Pedro A. Saa, Lisbel Bárzaga, Manuel A. Duarte-Mermoud, Mario Fernández-Fernández, Eduardo Agosin & José Ricardo Pérez-Correa. (2021) Robust control of fed-batch high-cell density cultures: a simulation-based assessment. Computers & Chemical Engineering 155, pages 107545.
Crossref
Talita G.S. Ramos, Fernanda Justen, Clara V.G.C. Carneiro, Victor M. Honorato, Paula F. Franco, Flávia S. Vieira, Débora Trichez, Clenilson M. Rodrigues & João R.M. Almeida. (2021) Xylonic acid production by recombinant Komagataella phaffii strains engineered with newly identified xylose dehydrogenases. Bioresource Technology Reports 16, pages 100825.
Crossref
Deepti Yadav, Bibhuti Ranjan, Nokuthula Mchunu, Marilize Le Roes-Hill & Tukayi Kudanga. (2021) Enhancing the expression of recombinant small laccase in Pichia pastoris by a double promoter system and application in antibiotics degradation. Folia Microbiologica 66:6, pages 917-930.
Crossref
Denggang Wang, Wenjie Li, Xinying Zhang, Shuli Liang & Ying Lin. (2021) Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation. Frontiers in Bioengineering and Biotechnology 9.
Crossref
Jin-Hua Mou, Imen Ben Tahar, Zhen-Yao Wang, Khai Lun Ong, Chong Li, Zi-Hao Qin, Xiang Wang, Carol Sze Ki Lin & Patrick Fickers. (2021) Enhancing the recombinant protein productivity of Yarrowia lipolytica using insitu fibrous bed bioreactor. Bioresource Technology 340, pages 125672.
Crossref
Julia Jodlbauer, Thomas Rohr, Oliver Spadiut, Marko D. Mihovilovic & Florian Rudroff. (2021) Biocatalysis in Green and Blue: Cyanobacteria. Trends in Biotechnology 39:9, pages 875-889.
Crossref
Ting Miao, Abdul Basit, Jiaqi Wen, Junquan Liu, Fengzhen Zheng, Yunhe Cao & Wei Jiang. (2021) High efficient degradation of glucan/glucomannan to cello-/mannan-oligosaccharide by endoglucanase via tetrasaccharide as intermediate. Food Chemistry 350, pages 129175.
Crossref
Anurag S. Rathore, Somesh Mishra, Saxena Nikita & Priyanka Priyanka. (2021) Bioprocess Control: Current Progress and Future Perspectives. Life 11:6, pages 557.
Crossref
Zeynep Efsun Duman-Özdamar & Barış Binay. (2021) Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects. The Protein Journal 40:3, pages 367-376.
Crossref
Hana Raschmanová, Astrid Weninger, Zdeněk Knejzlík, Karel Melzoch & Karin Kovar. (2021) Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins. Applied Microbiology and Biotechnology 105:11, pages 4397-4414.
Crossref
Gavino Carboni, Ivana Marova, Giacomo Zara, Severino Zara, Marilena Budroni & Ilaria Mannazzu. (2021) Evaluation of Recombinant Kpkt Cytotoxicity on HaCaT Cells: Further Steps towards the Biotechnological Exploitation Yeast Killer Toxins. Foods 10:3, pages 556.
Crossref
Yapeng Wang, Jianguang Lu, Zongqing Huang, Mengxin Qian, Qingbin Zhang & Jun Feng. (2021) Process development of recombinant Aspergillus flavus urate oxidase production in Pichia pastoris intracellularly and its characterization as a potential biosimilar. Process Biochemistry 102, pages 376-385.
Crossref
Wenchao Liu, Min Zhang, Bhesh Bhandari & Dongxing Yu. (2021) A novel combination of LF-NMR and NIR to intelligent control in pulse-spouted microwave freeze drying of blueberry. LWT 137, pages 110455.
Crossref
Juan David Rosas Cabrera & Carlos Andrés Díaz Rodríguez. (2021) Pichia pastoris as a biological platform to produce recombinant proteins: relevance for development of biotechnological products in Colombia. Revista de la Facultad de Ciencias 10:1, pages 20-44.
Crossref
Leidy D. Ardila-Leal, Raul A. Poutou-Piñales, Edwin D. Morales-Álvarez, Claudia M. Rivera-Hoyos, Aura M. Pedroza-Rodríguez, Balkys E. Quevedo-Hidalgo & Alejandro Pérez-Flórez. (2021) Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit. SN Applied Sciences 3:1.
Crossref
Gavino Carboni, Francesco Fancello, Giacomo Zara, Severino Zara, Luca Ruiu, Ivana Marova, Giovanni Pinna, Marilena Budroni & Ilaria Mannazzu. (2020) Production of a lyophilized ready-to-use yeast killer toxin with possible applications in the wine and food industries. International Journal of Food Microbiology 335, pages 108883.
Crossref
Wancang Liu, Haibo Xiang, Tao Zhang, Xu Pang, Jing Su, Hongyu Liu, Baiping Ma & Liyan Yu. (2020) Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris. Frontiers in Microbiology 11.
Crossref
Zeynep Efsun Duman, Bedri Burak Duraksoy, Fatih Aktaş, John M. Woodley & Barış Binay. (2020) High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris. Enzyme and Microbial Technology 137, pages 109552.
Crossref
Lucile Guyot, Lucie Hartmann, Sarah Mohammed‐Bouteben, Lydia Caro & Renaud Wagner. (2020) Preparation of Recombinant Membrane Proteins from Pichia pastoris for Molecular Investigations . Current Protocols in Protein Science 100:1.
Crossref
Emmanuelle Lautié, Olivier Russo, Pierre Ducrot & Jean A. Boutin. (2020) Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes. Frontiers in Pharmacology 11.
Crossref
Abraham Efraim Rodríguez-Mata, Ricardo Luna, Jose Ricardo Pérez-Correa, Alejandro Gonzalez-Huitrón, Rafael Castro-Linares & Manuel A. Duarte-Mermoud. (2020) Fractional Sliding Mode Nonlinear Procedure for Robust Control of an Eutrophying Microalgae Photobioreactor. Algorithms 13:3, pages 50.
Crossref
Ahmad Beiroti, Seyed Nezamedin Hosseini, Mohammad Reza Aghasadeghi & Dariush Norouzian. (2019) Comparative study of μ ‐stat methanol feeding control in fed‐batch fermentation of Pichia pastoris producing HBsAg: an open‐loop control versus recurrent artificial neural network‐based feedback control . Journal of Chemical Technology & Biotechnology 94:12, pages 3924-3931.
Crossref
Wan‐cang Liu, Fei Zhou, Di Xia & Joseph Shiloach. (2019) Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism . Microbial Biotechnology 12:6, pages 1226-1236.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.