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Bioscience, Biotechnology, and Biochemistry Volume 78, 2014 - Issue 1
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Biochemistry & Molecular Biology (Notes)

Stabilization of Moloney murine leukemia virus reverse transcriptase by site-directed mutagenesis of surface residue Val433

Atsushi KonishiDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
,
Xiaochen MaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
&
Kiyoshi YasukawaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, JapanCorrespondence[email protected]
Pages 75-78 | Received 13 Sep 2013, Accepted 02 Oct 2013, Published online: 15 Apr 2014

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Yuta Katano, Tongyang Li, Misato Baba, Miyo Nakamura, Masaaki Ito, Kenji Kojima, Teisuke Takita & Kiyoshi Yasukawa. (2017) Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system. Bioscience, Biotechnology, and Biochemistry 81:12, pages 2339-2345.
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Articles from other publishers (7)

Javier Martínez del Río, Nerea López-Carrobles, Jesús I. Mendieta-Moreno, Óscar Herrera-Chacón, Adrián Sánchez-Ibáñez, Jesús Mendieta & Luis Menéndez-Arias. (2023) Charge engineering of the nucleic acid binding cleft of a thermostable HIV-1 reverse transcriptase reveals key interactions and a novel mechanism of RNase H inactivation. Journal of Molecular Biology, pages 168219.
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A. A. Arteaga-Castrejón, M. R. Trejo-Hernández, Y. Mekmouche, A. Amouric, P. Rousselot-Pailley, V. Robert, T. Tron & F. Martínez-Morales. (2022) Relevance of Surface-Exposed Lysine Residues Designed for Functionalization of Laccase. Molecular Biology 56:4, pages 600-609.
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Igor P. Oscorbin & Maxim L. Filipenko. (2021) M-MuLV reverse transcriptase: Selected properties and improved mutants. Computational and Structural Biotechnology Journal 19, pages 6315-6327.
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Ryan C Heller, Suhman Chung, Katarzyna Crissy, Kyle Dumas, David Schuster & Thomas W Schoenfeld. (2019) Engineering of a thermostable viral polymerase using metagenome-derived diversity for highly sensitive and specific RT-PCR. Nucleic Acids Research 47:7, pages 3619-3630.
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S Palikša, G Alzbutas & R Skirgaila. (2018) Decreased Km to dNTPs is an essential M-MuLV reverse transcriptase adoption required to perform efficient cDNA synthesis in One-Step RT-PCR assay. Protein Engineering, Design and Selection 31:3, pages 79-89.
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Hiroyuki Okano, Misato Baba, Katsuhiro Kawato, Ryota Hidese, Itaru Yanagihara, Kenji Kojima, Teisuke Takita, Shinsuke Fujiwara & Kiyoshi Yasukawa. (2018) High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies. Journal of Bioscience and Bioengineering 125:3, pages 275-281.
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Atsushi Konishi, Tetsuro Hisayoshi, Kanta Yokokawa, Verónica Barrioluengo, Luis Menéndez-Arias & Kiyoshi Yasukawa. (2014) Amino acid substitutions away from the RNase H catalytic site increase the thermal stability of Moloney murine leukemia virus reverse transcriptase through RNase H inactivation. Biochemical and Biophysical Research Communications 454:2, pages 269-274.
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