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International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine Volume 48, 1985 - Issue 4
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Original Article

125I-induced DNA Double Strand Breaks: Use in Calibration of the Neutral Filter Elution Technique and Comparison with X-ray Induced Breaks

Ian R. Radford Biological Research Unit, Cancer Institute, 481 Little Lonsdale Street, Melbourne, Victoria, 3000, Australia
&
George S. Hodgson Biological Research Unit, Cancer Institute, 481 Little Lonsdale Street, Melbourne, Victoria, 3000, Australia
Pages 555-566 | Received 22 Nov 1984, Accepted 25 Mar 1985, Published online: 03 Jul 2009

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Amin I. Kassis. (2004) The Amazing World of Auger Electrons. International Journal of Radiation Biology 80:11-12, pages 789-803.
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K. M. PRISE, G. AHNSTRÖM, M. BELLI, J. CARLSSON, D. FRANKENBERG, J. KIEFER, M. LOÖBRICH, B. D. MICHAEL, J. NYGREN, G. SIMONE & B. STENERLÖW. (1998) A review of dsb induction data for varying quality radiations. International Journal of Radiation Biology 74:2, pages 173-184.
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G. LUDWIKOW. (1996) The effect of 125I decay at different stages of S-phase on survival, expression of micronuclei and chromosome aberrations in CHO cells. International Journal of Radiation Biology 70:2, pages 177-187.
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K.H. Chadwick & H.P. Leenhouts. (1994) On the Linearity of the Dose—effect Relationship of DNA Double Strand Breaks. International Journal of Radiation Biology 66:5, pages 549-552.
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M. Löbrich, S. Ikpeme & J. Kiefer. (1994) DNA Double-strand Break Measurement in Mammalian Cells by Pulsed-field Gel Electrophoresis: An Approach Using Restriction Enzymes and Gene Probing. International Journal of Radiation Biology 65:6, pages 623-630.
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I.R. Radford. (1994) Phorbol Esters Can Protect Mouse Pre-T Cell Lines from Radiation-induced Rapid Interphase Apoptosis. International Journal of Radiation Biology 65:3, pages 345-355.
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I.R. Radford. (1994) Radiation Response of Mouse Lymphoid and Myeloid Cell Lines. Part I. Sensitivity to Killing by Ionizing Radiation, Rate of Loss of Viability, and Cell Type of Origin. International Journal of Radiation Biology 65:2, pages 203-215.
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M. Flentje, B. Asadpour, D. Latz & K.J. Weber. (1993) Sensitivity of Neutral Filter Elution but Not PFGE Can Be Modified by Non-dsb Chromatin Damage. International Journal of Radiation Biology 63:6, pages 715-724.
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P.L. Olive & J.P. Banáth. (1993) Detection of DNA Double-strand Breaks through the Cell Cycle after Exposure to X-rays, Bleomycin, Etoposide and 125IdUrd. International Journal of Radiation Biology 64:4, pages 349-358.
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K.J. Weber & M. Flentje. (1993) Lethality of Heavy Ion-induced DNA Double-strand Breaks in Mammalian Cells. International Journal of Radiation Biology 64:2, pages 169-178.
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C. Baumstark-Khan. (1993) X-ray-induced DNA Double-strand Breaks as Lethal Lesions in Diploid Human Fibroblasts Compared to Chinese Hamster Ovary Cells. International Journal of Radiation Biology 63:3, pages 305-311.
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M.D. Story, K.C. Garrett, P.J. Tofilon & R.E. Meyn. (1993) Influence of Irradiation Conditions on the Measurement of DNA Double-strand Breaks by Pulsed-field Gel Electrophoresis. International Journal of Radiation Biology 63:3, pages 297-304.
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A.A. Friedl, W. Beisker, K. Hahn, F. Eckardt-Schupp & A.M. Kellerer. (1993) Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules. International Journal of Radiation Biology 63:2, pages 173-181.
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J. Nygren & G. Ahnström. (1992) The Filter Does Not Act as a DNA Size Discriminator in the Neutral Filter Elution Technique. International Journal of Radiation Biology 62:4, pages 397-399.
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G. Luduikow, F. Ludwikow & K.J. Johanson. (1992) Kinetics of Micronucleus Induction by 125I-labelled Thyroid Hormone in Hormone-responsive Cells. International Journal of Radiation Biology 61:5, pages 639-653.
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C. Baumstark-Khan. (1992) Effect of Aphidicolin on DNA Synthesis, PLD-recovery and DNA Repair of Human Diploid Fibroblasts. International Journal of Radiation Biology 61:2, pages 191-197.
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M.J. Peak, L. Wang, C.K. Hill & J.G. Peak. (1991) Comparison of Repair of DNA Double-strand Breaks Caused by Neutron or Gamma Radiation in Cultured Human Cells. International Journal of Radiation Biology 60:6, pages 891-898.
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O. Sapora, F. Barone, M. Belli, A. Maggi, M. Quintiliani & M.A. Tabocchini. (1991) Relationships Between Cell Killing, Mutation Induction and DNA Damage in X-irradiated V79 Cells: The Influence of Oxygen and DMSO. International Journal of Radiation Biology 60:3, pages 467-482.
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I.R. Radford. (1991) Mouse Lymphoma Cells that Undergo Interphase Death Show Markedly Increased Sensitivity to Radiation-induced DNA Double-strand Breakage as Compared with Cells that Undergo Mitotic Death. International Journal of Radiation Biology 59:6, pages 1353-1369.
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G.E. Iliakis, O. Cicilioni & L. Metzger. (1991) Measurement of DNA Double-strand Breaks in CHO Cells at Various Stages of the Cell Cycle Using Pulsed Field Gel Electrophoresis: Calibration by Means of 125I Decay. International Journal of Radiation Biology 59:2, pages 343-357.
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G.E. Iliakis, L. Metzger, N. Denko & T.D. Stamato. (1991) Detection of DNA Double-strand Breaks in Synchronous Cultures of CHO Cells by Means of Asymmetric Field Inversion Gel Electrophoresis. International Journal of Radiation Biology 59:2, pages 321-341.
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K. Eguchi-Kasai, T. Kosaka, K. Sato & I. Kaneko. (1991) Reparability of DNA Double-strand Breaks and Radiation Sensitivity in Five Mammalian Cell Lines. International Journal of Radiation Biology 59:1, pages 97-104.
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I.R. Radford. (1990) Rapid Communication. International Journal of Radiation Biology 57:3, pages 479-483.
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K.M. Prise, M. Folkard, S. Davies & B.D. Michael. (1990) The Irradiation of V79 Mammalian Cells by Protons with Energies below 2 MeV. Part II. Measurement of Oxygen Enhancement Ratios and DNA Damage. International Journal of Radiation Biology 58:2, pages 261-277.
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D.D. Ager & W.C. Dewey. (1990) Calibration of Pulsed Field Gel Electrophoresis for Measurement of DNA Double-strand Breaks. International Journal of Radiation Biology 58:2, pages 249-259.
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J.F. Ward. (1990) The Yield of DNA Double-strand Breaks Produced Intracellularly by Ionizing Radiation: A Review. International Journal of Radiation Biology 57:6, pages 1141-1150.
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G.W. Barendsen. (1990) Mechanisms of Cell Reproductive Death and Shapes of Radiation Dose-survival Curves of Mammalian Cells. International Journal of Radiation Biology 57:4, pages 885-896.
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D. Blöcher. (1990) In CHEF Electrophoresis a Linear Induction of Dsb Corresponds to a Nonlinear Fraction of Extracted DNA with Dose. International Journal of Radiation Biology 57:1, pages 7-12.
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K.M. Prise, S. Davies & B.D. Michael. (1989) Non-linear Dose—effect Curve for DNA Double-strand Breaks by Low LET Radiation: The Effect of Eluting Buffer Composition on the Measurement of Breaks by the Filter Elution Technique. International Journal of Radiation Biology 56:6, pages 943-950.
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D.E. Charlton, H. Nikjoo & J.L. Humm. (1989) Calculation of Initial Yields of Single- and Double-strand Breaks in Cell Nuclei from Electrons, Protons and Alpha Particles. International Journal of Radiation Biology 56:1, pages 1-19.
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Ryuichi Okayasu & George Iliakis. (1989) Linear DNA Elution Dose Response Curves Obtained in CHO Cells with Non-unwinding Filter Elution after Appropriate Selection of the Lysis Conditions. International Journal of Radiation Biology 55:4, pages 569-581.
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Kevin M. Prise, Susan Davies & Barry D. Michael. (1989) An Improved Method for the Treatment of Data from DNA Strand Break Measurements Using Filter Elution Techniques with an Internal Standard. International Journal of Radiation Biology 55:3, pages 323-330.
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George Iliakis & Ryuichi Okayasu. (1988) The Level of Induced DNA Double-strand Breaks Does Not Correlate with Cell Killing in X-irradiated Mitotic and G1-phase CHO Cells. International Journal of Radiation Biology 53:3, pages 395-404.
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D.E. Charlton & J.L. Humm. (1988) A Method of Calculating Initial DNA Strand Breakage Following the Decay of Incorporated 125I. International Journal of Radiation Biology 53:3, pages 353-365.
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Thomas M. Koval & Elizabeth R. Kazmar. (1988) Eluting Solution Composition Affects DNA Double-strand Break Analysis by Filter Elution. International Journal of Radiation Biology 54:5, pages 739-747.
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Ian R. Radford, George S. Hodgson & Jane P. Matthews. (1988) Critical DNA Target Size Model of Ionizing Radiation-induced Mammalian Cell Death. International Journal of Radiation Biology 54:1, pages 63-79.
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George Iliakis, Ryuichi Okayasu & Robert Seaner. (1988) Radiosensitive Xrs-5 and Parental CHO Cells Show Identical DNA Neutral Filter Elution Dose—response: Implications for a Relationship between Cell Radiosensitivity and Induction of DNA Double-strand Breaks. International Journal of Radiation Biology 54:1, pages 55-62.
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Ian R. Radford. (1988) The Dose—response for Low-LET Radiation-induced DNA Double-strand Breakage: Methods of Measurement and Implications for Radiation Action Models. International Journal of Radiation Biology 54:1, pages 1-11.
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Ryuichi Okayasu, Detlef Bloecher & George Iliakis. (1988) Variation through the Cell Cycle in the Dose-response of DNA Neutral Filter Elution in X-irradiated Synchronous CHO-cells. International Journal of Radiation Biology 53:5, pages 729-747.
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Ian R. Radford & Sam Broadhurst. (1988) Aphidicolin Synchronization of Mouse L Cells Perturbs the Relationship between Cell Killing and DNA Double-strand Breakage after X-irradiation. International Journal of Radiation Biology 53:2, pages 205-215.
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Kevin M. Prise, Susan Davies & Barry D. Michael. (1987) The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or 238Pu α-particles. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 52:6, pages 893-902.
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Ian R. Radford. (1987) Use of ‘Nuclear Monolayers’ to Identify Factors Influencing DNA Double-Strand Breakage by X-rays. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 52:6, pages 853-858.
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George Iliakis, Gabriel E. Pantelias, Ryuichi Okayasu & Robert Seaner. (1987) 125IdUrd-induced Chromosome Fragments, Assayed by Premature Chromosome Condensation, and DNA Double-strand Breaks Have Similar Repair Kinetics in G1-phase CHO-cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 52:5, pages 705-722.
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Ian R. Radford. (1987) Effect of Cell-cycle Position and Dose on the Kinetics of DNA Double-strand Breakage Repair in X-irradiated Chinese Hamster Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 52:4, pages 555-563.
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Ian R. Radford & George S. Hodgson. (1987) Effect of Ploidy on the Response of V79 Cells to Ionizing Radiation. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 51:5, pages 765-778.
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Ian R. Radford & Sam Broadhurst. (1986) Enhanced Induction by X-irradiation of DNA Double-strand Breakage in Mitotic as Compared with S-phase V79 Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 49:6, pages 909-914.
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Ian R. Radford. (1985) Evidence for a General Relationship between the Induced Level of DNA Double-strand Breakage and Cell-killing after X-irradiation of Mammalian Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 49:4, pages 611-620.
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