1,497
Views
39
CrossRef citations to date
0
Altmetric
PAPERS

Insight into factors directing high production of eukaryotic membrane proteins; production of 13 human AQPs in Pichia pastoris

, , , , , , , & show all
Pages 215-227 | Received 16 Oct 2008, Published online: 09 Jul 2009

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (3)

Kristina Hedfalk. (2013) Further advances in the production of membrane proteins in . Bioengineered 4:6, pages 363-367.
Read now
Fredrik Öberg & Kristina Hedfalk. (2013) Recombinant production of the human aquaporins in the yeast Pichia pastoris (Invited Review). Molecular Membrane Biology 30:1, pages 15-31.
Read now
Fredrik Öberg, Jennie Sjöhamn, Matthew T. Conner, Roslyn M. Bill & Kristina Hedfalk. (2011) Improving recombinant eukaryotic membrane protein yields in Pichia pastoris: The importance of codon optimization and clone selection. Molecular Membrane Biology 28:6, pages 398-411.
Read now

Articles from other publishers (36)

Mechthild Stoeckelhuber, Florian D. Grill, Klaus-Dietrich Wolff, Marco R. Kesting, Constantin T. Wolff, Andreas M. Fichter, Denys J. Loeffelbein, Christoph Schmitz & Lucas M. Ritschl. (2023) Infantile human labial glands: Distribution of aquaporins and claudins in the context of paracellular and transcellular pathways. Tissue and Cell 82, pages 102052.
Crossref
Abouzar Azarafza, Muhammad Amirul Islam, Yekta Golpazirsorkheh, Irene Efteghar, Mohtada Sadrzadeh, Milad Kamkar, Arsalan Faghih Shojaei, Mohammad Younas, Tejraj M. Aminabhavi & Mashallah Rezakazemi. (2023) Aquaporin‐Based Biomimetic Membranes for Low Energy Water Desalination and Separation Applications. Advanced Functional Materials 33:21.
Crossref
Michael Groszmann, Annamaria De Rosa, Weihua Chen, Jiaen Qiu, Samantha A. McGaughey, Caitlin S. Byrt & John R. Evans. (2023) A high-throughput yeast approach to characterize aquaporin permeabilities: Profiling the Arabidopsis PIP aquaporin sub-family. Frontiers in Plant Science 14.
Crossref
Antonio Carlesso, Raquel Delgado, Oriol Ruiz Isant, Owens Uwangue, Dylan Valli, Roslyn M Bill & Kristina Hedfalk. (2022) Yeast as a tool for membrane protein production and structure determination. FEMS Yeast Research 22:1.
Crossref
Roslyn M. Bill & Kristina Hedfalk. (2021) Aquaporins – Expression, purification and characterization. Biochimica et Biophysica Acta (BBA) - Biomembranes 1863:9, pages 183650.
Crossref
Yuewen Zhang, Therese W. Herling, Stefan Kreida, Quentin A. E. Peter, Tadas Kartanas, Susanna Törnroth-Horsefield, Sara Linse & Tuomas P. J. Knowles. (2020) A microfluidic strategy for the detection of membrane protein interactions. Lab on a Chip 20:17, pages 3230-3238.
Crossref
Athanasios Kesidis, Peer Depping, Alexis Lodé, Afroditi Vaitsopoulou, Roslyn M. Bill, Alan D. Goddard & Alice J. Rothnie. (2020) Expression of eukaryotic membrane proteins in eukaryotic and prokaryotic hosts. Methods 180, pages 3-18.
Crossref
Rachel Munro, Jeffrey de Vlugt, Vladimir Ladizhansky & Leonid S. Brown. (2020) Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in Pichia pastoris for Solid-State NMR. Biomolecules 10:3, pages 434.
Crossref
Augusto Quaresma Pedro, João António Queiroz & Luís António Passarinha. (2019) Smoothing membrane protein structure determination by initial upstream stage improvements. Applied Microbiology and Biotechnology 103:14, pages 5483-5500.
Crossref
P.M. Gotovtsev, A.V. Komova, K.V. Gorin, Ya E. Sergeeva, I.A. Konova & R.G. Vasilov. (2019) Biotechnology for thermal power plants. A review of recent and perspective technologies. Sustainable Energy Technologies and Assessments 31, pages 132-141.
Crossref
Amira Abdelrasoul, Huu Doan, Ali Lohi & Chil-Hung Cheng. (2018) Aquaporin-Based Biomimetic and Bioinspired Membranes for New Frontiers in Sustainable Water Treatment Technology: Approaches and Challenges. Polymer Science, Series A 60:4, pages 429-450.
Crossref
Xuefeng Zhang, Yun Chen, Liming Dong & Benkang Shi. (2018) Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells. Oncology Letters.
Crossref
Stefan Kreida, Jennifer Virginia Roche, Caroline Olsson, Sara Linse & Susanna Törnroth-Horsefield. (2018) Protein–protein interactions in AQP regulation – biophysical characterization of AQP0–CaM and AQP2–LIP5 complex formation. Faraday Discussions 209, pages 35-54.
Crossref
Amira Abdelrasoul, Huu Doan & Ali Lohi. 2017. Biomimetic and Bioinspired Membranes for New Frontiers in Sustainable Water Treatment Technology. Biomimetic and Bioinspired Membranes for New Frontiers in Sustainable Water Treatment Technology.
Frederik Bühring Bjørkskov, Simon Lyngaa Krabbe, Casper Normann Nurup, Julie Winkel Missel, Mariana Spulber, Julie Bomholt, Karen Molbaek, Claus Helix-Nielsen, Kamil Gotfryd, Pontus Gourdon & Per Amstrup Pedersen. (2017) Purification and functional comparison of nine human Aquaporins produced in Saccharomyces cerevisiae for the purpose of biophysical characterization. Scientific Reports 7:1.
Crossref
Jennie Sjöhamn, Petra Båth, Richard Neutze & Kristina Hedfalk. (2016) Applying bimolecular fluorescence complementation to screen and purify aquaporin protein:protein complexes. Protein Science 25:12, pages 2196-2208.
Crossref
Sarah J. Routledge, Lina Mikaliunaite, Anjana Patel, Michelle Clare, Stephanie P. Cartwright, Zharain Bawa, Martin D.B. Wilks, Floren Low, David Hardy, Alice J. Rothnie & Roslyn M. Bill. (2016) The synthesis of recombinant membrane proteins in yeast for structural studies. Methods 95, pages 26-37.
Crossref
Stephanie P. Cartwright, Lina Mikaliunaite & Roslyn M. Bill. 2016. Heterologous Expression of Membrane Proteins. Heterologous Expression of Membrane Proteins 23 35 .
Mustafa Erbakan, Brandon S. Curtis, B. Tracy Nixon, Manish Kumar & Wayne R. Curtis. (2015) Advancing Rhodobacter sphaeroides as a platform for expression of functional membrane proteins. Protein Expression and Purification 115, pages 109-117.
Crossref
Roslyn M Bill & Tobias von der Haar. (2015) Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects. Current Opinion in Structural Biology 32, pages 147-155.
Crossref
A. Q. Pedro, D. Oppolzer, M. J. Bonifácio, C. J. Maia, J. A. Queiroz & L. A. Passarinha. (2015) Evaluation of MutS and Mut+ Pichia pastoris Strains for Membrane-Bound Catechol-O-Methyltransferase Biosynthesis. Applied Biochemistry and Biotechnology 175:8, pages 3840-3855.
Crossref
Ying Fan, Sanaz Emami, Rachel Munro, Vladimir Ladizhansky & Leonid S. Brown. 2015. Isotope Labeling of Biomolecules - Labeling Methods. Isotope Labeling of Biomolecules - Labeling Methods 193 212 .
Anita Emmerstorfer, Tamara Wriessnegger, Melanie Hirz & Harald Pichler. (2014) Overexpression of membrane proteins from higher eukaryotes in yeasts. Applied Microbiology and Biotechnology 98:18, pages 7671-7698.
Crossref
Piero Pingitore, Carlo Pirazzi, Rosellina M. Mancina, Benedetta M. Motta, Cesare Indiveri, Arturo Pujia, Tiziana Montalcini, Kristina Hedfalk & Stefano Romeo. (2014) Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of function. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1841:4, pages 574-580.
Crossref
Nadia Gul, Daniel M. Linares, Franz Y. Ho & Bert Poolman. (2014) Evolved Escherichia coli Strains for Amplified, Functional Expression of Membrane Proteins. Journal of Molecular Biology 426:1, pages 136-149.
Crossref
Annika Müller-Lucks, Patrizia Gena, Daniele Frascaria, Nicola Altamura, Maria Svelto, Eric Beitz & Giuseppe Calamita. (2013) Preparative scale production and functional reconstitution of a human aquaglyceroporin (AQP3) using a cell free expression system. New Biotechnology 30:5, pages 545-551.
Crossref
Sanaz Emami, Ying Fan, Rachel Munro, Vladimir Ladizhansky & Leonid S. Brown. (2013) Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra. Journal of Biomolecular NMR 55:2, pages 147-155.
Crossref
C.Y. Tang, Y. Zhao, R. Wang, C. Hélix-Nielsen & A.G. Fane. (2013) Desalination by biomimetic aquaporin membranes: Review of status and prospects. Desalination 308, pages 34-40.
Crossref
Linnéa Isaksson, Johan Enberg, Richard Neutze, B. Göran Karlsson & Anders Pedersen. (2012) Expression screening of membrane proteins with cell-free protein synthesis. Protein Expression and Purification 82:1, pages 218-225.
Crossref
Kristina Hedfalk. 2012. Recombinant Protein Production in Yeast. Recombinant Protein Production in Yeast 47 55 .
Fredrik Öberg, Jennie Sjöhamn, Gerhard Fischer, Andreas Moberg, Anders Pedersen, Richard Neutze & Kristina Hedfalk. (2011) Glycosylation Increases the Thermostability of Human Aquaporin 10 Protein. Journal of Biological Chemistry 286:36, pages 31915-31923.
Crossref
Fatima Alkhalfioui, Christel Logez, Olivier Bornert & Renaud Wagner. 2011. Production of Membrane Proteins. Production of Membrane Proteins 75 108 .
Ana Ramón & Mónica Marín. (2011) Advances in the production of membrane proteins in Pichia pastoris. Biotechnology Journal 6:6, pages 700-706.
Crossref
Ying Fan, Lichi Shi, Vladimir Ladizhansky & Leonid S. Brown. (2011) Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environment. Journal of Biomolecular NMR 49:2, pages 151-161.
Crossref
Nicola Altamura & Giuseppe Calamita. 2012. Biomimetic Membranes for Sensor and Separation Applications. Biomimetic Membranes for Sensor and Separation Applications 233 250 .
Susanna Törnroth-Horsefield, Kristina Hedfalk, Gerhard Fischer, Karin Lindkvist-Petersson & Richard Neutze. (2010) Structural insights into eukaryotic aquaporin regulation. FEBS Letters 584:12, pages 2580-2588.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.