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Original Article

Topology-informed strategies for the overexpression and purification of membrane proteins

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Pages 407-418 | Received 16 Oct 2006, Published online: 09 Jul 2009

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Read on this site (4)

Xiaobing Xia, Vincent L. G. Postis, Moazur Rahman, Gareth S. A. Wright, Peter C. J. Roach, Sarah E. Deacon, Jean C. Ingram, Peter J. F. Henderson, John B. C. Findlay, Simon E. V. Phillips, Vincent L. G. Postis, Michael J. McPherson & Stephen A. Baldwin. (2008) Investigation of the structure and function of a Shewanella oneidensis arsenical-resistance family transporter. Molecular Membrane Biology 25:8, pages 691-701.
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Peter C. J. Roach, Vincent L. G. Postis, Sarah E. Deacon, Gareth S. A. Wright, Jean C. Ingram, Xiaobing Xia, Michael J. McPherson & Stephen A. Baldwin. (2008) Large-scale preparation of bacterial cell membranes by tangential flow filtration. Molecular Membrane Biology 25:8, pages 609-616.
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Sarah E. Deacon, Peter C. J. Roach, Vincent L.G. Postis, Gareth S. A. Wright, Xiaobing Xia, Simon E. V. Phillips, J. Paul Knox, Peter J. F. Henderson, Michael J. McPherson & Stephen A. Baldwin. (2008) Reliable scale-up of membrane protein over-expression by bacterial auto-induction: From microwell plates to pilot scale fermentations. Molecular Membrane Biology 25:8, pages 588-598.
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Moazur Rahman, Simon G. Patching, Fouzia Ismat, Peter J. F. Henderson, Richard B. Herbert, Stephen A. Baldwin & Michael J. McPherson. (2008) Probing metal ion substrate-binding to the E. coli ZitB exporter in native membranes by solid state NMR. Molecular Membrane Biology 25:8, pages 683-690.
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Articles from other publishers (27)

Cheng Ma & Caixia Gong. 2023. 1 30 .
Aqsa Shaheen, Fouzia Ismat, Mazhar Iqbal, Abdul Haque, Zaheer Ul‐Haq, Osman Mirza, Rita De Zorzi, Thomas Walz & Moazur Rahman. (2021) Characterization of the multidrug efflux transporter styMdtM from Salmonella enterica serovar Typhi . Proteins: Structure, Function, and Bioinformatics 89:9, pages 1193-1204.
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Jiyoung Shim, Chuqing Zhou, Ting Gong, Dasha Aleksandra Iserlis, Hamad Abdullah Linjawi, Matthew Wong, Tingrui Pan & Cheemeng Tan. (2021) Building protein networks in synthetic systems from the bottom-up. Biotechnology Advances 49, pages 107753.
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Sarah Spruce Preisler, Anders Drabaek Wiuf, Marc Friis, Lasse Kjaergaard, Molly Hurd, Eva Ramos Becares, Casper Normann Nurup, Frederik Bühring Bjoerkskov, Zsófia Szathmáry, Pontus Emanuel Gourdon, Kirstine Calloe, Dan A. Klaerke, Kamil Gotfryd & Per Amstrup Pedersen. (2021) Saccharomyces cerevisiae as a superior host for overproduction of prokaryotic integral membrane proteins. Current Research in Structural Biology 3, pages 51-71.
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Maryam Zafar, Majid Ali Shah, Aamir Shehzad, Anam Tariq, Mudasser Habib, Muhammad Muddassar, Muhammad Salahuddin Shah, Mazhar Iqbal, Farhid Hemmatzadeh & Moazur Rahman. (2020) Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family. Applied Microbiology and Biotechnology 104:8, pages 3391-3402.
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Yixin Liu, Ana Pavić, Joshua T. Farley, Carine de Marcos Lousa, Adrian Goldman & Vincent L. G. Postis. 2020. Biophysics of Membrane Proteins. Biophysics of Membrane Proteins 3 49 .
Hongmin Cai, Hebang Yao, Tingting Li, Yannan Tang & Dianfan Li. (2019) High-level heterologous expression of the human transmembrane sterol Δ8,Δ7-isomerase in Pichia pastoris. Protein Expression and Purification 164, pages 105463.
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Augusto Quaresma Pedro, João António Queiroz & Luís António Passarinha. (2019) Smoothing membrane protein structure determination by initial upstream stage improvements. Applied Microbiology and Biotechnology 103:14, pages 5483-5500.
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Aalishaa A. Azam, Jean M. Kinder, G. Nasir Khan, Ade Alase, Pikyee Ma, Yang Liu, James R. Ault, Peter J. F. Henderson, Babur Z. Chowdhry, Bruce D. Alexander, Stephen E. Harding & Mary K. Phillips-Jones. (2018) Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions. European Biophysics Journal 47:7, pages 723-737.
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Marvin V. Dilworth, Mathilde S. Piel, Kim E. Bettaney, Pikyee Ma, Ji Luo, David Sharples, David R. Poyner, Stephane R. Gross, Karine Moncoq, Peter J.F. Henderson, Bruno Miroux & Roslyn M. Bill. (2018) Microbial expression systems for membrane proteins. Methods 147, pages 3-39.
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V. Vasilca, A. Sadeghpour, S. Rawson, L.E. Hawke, S.A. Baldwin, T. Wilkinson, D. Bannister, V.L.G. Postis, M. Rappolt, S.P. Muench & L.J.C. Jeuken. (2018) Spherical-supported membranes as platforms for screening against membrane protein targets. Analytical Biochemistry 549, pages 58-65.
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Moazur Rahman, Fouzia Ismat, Li Jiao, Jocelyn M. Baldwin, David J. Sharples, Stephen A. Baldwin & Simon G. Patching. (2016) Characterisation of the DAACS Family Escherichia coli Glutamate/Aspartate-Proton Symporter GltP Using Computational, Chemical, Biochemical and Biophysical Methods. The Journal of Membrane Biology 250:2, pages 145-162.
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Yanke Chen, Qichang Li, Jun Yang & Hao Xie. (2016) Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease. Applied Biochemistry and Biotechnology 181:3, pages 939-947.
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Aditya PandeyKyungsoo ShinRobin E. PattersonXiang-Qin LiuJan K. Rainey. (2016) Current strategies for protein production and purification enabling membrane protein structural biology. Biochemistry and Cell Biology 94:6, pages 507-527.
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Anasztázia Hetényi, Zsófia Hegedűs, Roberta Fajka-Boja, Éva Monostori, Katalin E. Kövér & Tamás A. Martinek. (2016) Target-specific NMR detection of protein–ligand interactions with antibody-relayed 15N-group selective STD. Journal of Biomolecular NMR 66:4, pages 227-232.
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Cheng Ma, Zhenyu Hao, Gerard Huysmans, Amelia Lesiuk, Per Bullough, Yingying Wang, Mark Bartlam, Simon E. Phillips, James D. Young, Adrian Goldman, Stephen A. Baldwin & Vincent L. G. Postis. (2015) A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters. PLOS ONE 10:11, pages e0143010.
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Aqsa Shaheen, Fouzia Ismat, Mazhar Iqbal, Abdul Haque, Rita De Zorzi, Osman Mirza, Thomas Walz & Moazur Rahman. (2015) Characterization of putative multidrug resistance transporters of the major facilitator-superfamily expressed in Salmonella Typhi. Journal of Infection and Chemotherapy 21:5, pages 357-362.
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Masao Yamashita, Mark Shepherd, Wesley I. Booth, Hao Xie, Vincent Postis, Yvonne Nyathi, Svetomir B. Tzokov, Robert K. Poole, Stephen A. Baldwin & Per A. Bullough. (2014) Structure and Function of the Bacterial Heterodimeric ABC Transporter CydDC. Journal of Biological Chemistry 289:33, pages 23177-23188.
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Bala K. Prabhala, Nanda G. Aduri, Johanne M. Jensen, Heidi A. Ernst, Nida Iram, Moazur Rahman & Osman Mirza. (2014) New insights into the substrate specificities of proton‐coupled oligopeptide transporters from E. coli by a pH sensitive assay . FEBS Letters 588:4, pages 560-565.
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Pikyee Ma, Filipa Varela, Malgorzata Magoch, Ana Rita Silva, Ana Lúcia Rosário, José Brito, Tânia Filipa Oliveira, Przemyslaw Nogly, Miguel Pessanha, Meike Stelter, Arnulf Kletzin, Peter J. F. Henderson & Margarida Archer. (2013) An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli. PLoS ONE 8:10, pages e76913.
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Hae Joo Kang, Chiara Lee & David Drew. (2013) Breaking the barriers in membrane protein crystallography. The International Journal of Biochemistry & Cell Biology 45:3, pages 636-644.
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Vincent G. L. Postis, Andrea E. Rawlings, Amelia Lesiuk & Stephen A. Baldwin. 2013. Ion Channels. Ion Channels 33 54 .
Gerard H. M. HuysmansNathan ChanJocelyn M. BaldwinVincent L. G. PostisSvetomir B. TzokovSarah E. DeaconSylvia Y. M. Yao, James D. Young, Michael J. McPhersonPer A. BulloughStephen A. Baldwin. (2012) A urea channel from Bacillus cereus reveals a novel hexameric structure . Biochemical Journal 445:2, pages 157-166.
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Florent Bernaudat, Annie Frelet-Barrand, Nathalie Pochon, Sébastien Dementin, Patrick Hivin, Sylvain Boutigny, Jean-Baptiste Rioux, Daniel Salvi, Daphné Seigneurin-Berny, Pierre Richaud, Jacques Joyard, David Pignol, Monique Sabaty, Thierry Desnos, Eva Pebay-Peyroula, Elisabeth Darrouzet, Thierry Vernet & Norbert Rolland. (2011) Heterologous Expression of Membrane Proteins: Choosing the Appropriate Host. PLoS ONE 6:12, pages e29191.
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Annie Frelet-Barrand, Sylvain Boutigny, Edmund R. S. Kunji & Norbert Rolland. 2010. Heterologous Expression of Membrane Proteins. Heterologous Expression of Membrane Proteins 67 85 .
Heidi A. Ernst, Antony Pham, Helle Hald, Jette S. Kastrup, Moazur Rahman & Osman Mirza. (2009) Ligand binding analyses of the putative peptide transporter YjdL from E. coli display a significant selectivity towards dipeptides. Biochemical and Biophysical Research Communications 389:1, pages 112-116.
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Pascal Courville, Eva Urbankova, Christopher Rensing, Roman Chaloupka, Matthias Quick & Mathieu F.M. Cellier. (2008) Solute Carrier 11 Cation Symport Requires Distinct Residues in Transmembrane Helices 1 and 6. Journal of Biological Chemistry 283:15, pages 9651-9658.
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