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Preparative Biochemistry Volume 14, 1984 - Issue 1
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Original Articles

Large Scale Preparation and Characterization of Membrane-Bound and Detergent-Solubilized Muscarinic Acetylcholine Receptor from Pig Atria

Gary L. Peterson Department of Biochemistry and Biophysics, Oregon State University Corvallis, Oregon, 97331
&
Michael I. Schimerlik Department of Biochemistry and Biophysics, Oregon State University Corvallis, Oregon, 97331
Pages 33-74 | Published online: 13 Dec 2006

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Marcelo J. Alfonzo, Ramona González de Alfonzo, Marcelo Alfonzo González & Itala Lippo de Becemberg. (2015) Muscarinic drugs regulate the PKG-II-dependent phosphorylation of M3 muscarinic acetylcholine receptors at plasma membranes from airway smooth muscle. Journal of Receptors and Signal Transduction 35:4, pages 319-328.
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Marcelo J. Alfonzo, Ramona González De Alfonzo, Marcelo A. Alfonzo-González & Itala Lippo De Becemberg. (2013) Cyclic GMP regulates M3AChR activity at plasma membranes from Airway smooth muscle. Molecular Membrane Biology 30:8, pages 403-417.
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J. G. Van Gelderen, L. Daeffler, D. Scherrer, M. Mousli, Y. Landry & J. P. Gies. (1996) M2-Muscarinic Receptors: How Does Ligand Binding Affinity Relate to Intrinsic Activity?. Journal of Receptors and Signal Transduction 16:1-2, pages 135-148.
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J. Šmisterová, K. Ensing, J. de Boer & R.A. de Zeeuw. (1995) Evaluation of A Purification Procedure for the Muscarinic Receptor for the Purpose of Quantitative Receptor Assays of Anticholinergics. Preparative Biochemistry 25:4, pages 223-251.
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Articles from other publishers (36)

Rabindra V. Shivnaraine, Xi-Ping Huang, Margaret Seidenberg, John Ellis & James W. Wells. (2012) Heterotropic Cooperativity within and between Protomers of an Oligomeric M 2 Muscarinic Receptor . Biochemistry 51:22, pages 4518-4540.
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Luca F. Pisterzi, David B. Jansma, John Georgiou, Michael J. Woodside, Judy Tai-Chieh Chou, Stéphane Angers, Valerică Raicu & James W. Wells. (2010) Oligomeric Size of the M 2 Muscarinic Receptor in Live Cells as Determined by Quantitative Fluorescence Resonance Energy Transfer . Journal of Biological Chemistry 285:22, pages 16723-16738.
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Charles E. Whitehurst, Naim Nazef, D. Allen Annis, Yongmin Hou, Denise M. Murphy, Peter Spacciapoli, Zhiping Yao, Michael R. Ziebell, Cliff C. Cheng, Gerald W. Shipps, Jr.Jason S. Felsch, David Lau & Huw M. Nash. (2006) Discovery and Characterization of Orthosteric and Allosteric Muscarinic M2 Acetylcholine Receptor Ligands by Affinity Selection–Mass Spectrometry. SLAS Discovery 11:2, pages 194-207.
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Bin Li, Ellen G. Duysen, Laura A. Volpicelli-Daley, Allan I. Levey & Oksana Lockridge. (2003) Regulation of muscarinic acetylcholine receptor function in acetylcholinesterase knockout mice. Pharmacology Biochemistry and Behavior 74:4, pages 977-986.
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Chi Shing Sum, Paul S.-H. Park & James W. Wells. (2002) Effects of N-Ethylmaleimide on Conformational Equilibria in Purified Cardiac Muscarinic Receptors. Journal of Biological Chemistry 277:39, pages 36188-36203.
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Chi Shing Sum, Norman Pyo & James W. Wells. (2001) Apparent capacity of cardiac muscarinic receptors for different radiolabeled antagonists 6 6Abbreviations: ABT, 3-(2′-aminobenzhydryloxy)tropane; ECL, enhanced chemiluminescence; NMS, N-methylscopolamine; PMSF, phenylmethylsulfonyl fluoride; and QNB, l-quinuclidinylbenzilate.. Biochemical Pharmacology 62:7, pages 829-851.
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Joshua A. Bomser & John E. Casida. (2001) Diethylphosphorylation of rat cardiac M2 muscarinic receptor by chlorpyrifos oxon in vitro. Toxicology Letters 119:1, pages 21-26.
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Simon J. Evans, Thomas F. Murray & Frank L. Moore. (2000) Partial purification and biochemical characterization of a membrane glucocorticoid receptor from an amphibian brain. The Journal of Steroid Biochemistry and Molecular Biology 72:5, pages 209-221.
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Laurent Daeffler, Ahmed Chahdi, Jean‐Pierre Gies & Yves Landry. (2009) Inhibition of GTPase activity of G i proteins and decreased agonist affinity at M 2 muscarinic acetylcholine receptors by spermine and methoctramine . British Journal of Pharmacology 127:4, pages 1021-1029.
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Laurent Daeffler, Fabien Schmidlin, Jean‐Pierre Gies & Yves Landry. (2009) Inverse agonist activity of pirenzepine at M 2 muscarinic acetylcholine receptors . British Journal of Pharmacology 126:5, pages 1246-1252.
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Walter K. Vogel, Gary L. Peterson, David J. Broderick, Valerie A. Mosser & Michael I. Schimerlik. (1999) Double Mutant Cycle Analysis of Aspartate 69, 97, and 103 to Asparagine Mutants in the m2 Muscarinic Acetylcholine Receptor. Archives of Biochemistry and Biophysics 361:2, pages 283-294.
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Simon J Evans, Frank L Moore & Thomas F Murray. (1998) Solubilization and pharmacological characterization of a glucocorticoid membrane receptor from an amphibian brain. The Journal of Steroid Biochemistry and Molecular Biology 67:1, pages 1-8.
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Michael I. Schimerlik. (2001) Overview of Membrane Protein Solubilization. Current Protocols in Neuroscience 2:1.
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Walter K. Vogel, David M. Sheehan & Michael I. Schimerlik. (1997) Site-Directed Mutagenesis on the m2 Muscarinic Acetylcholine Receptor: The Significance of Tyr403 in the Binding of Agonists and Functional Coupling. Molecular Pharmacology 52:6, pages 1087-1094.
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Keith A. Wreggett & James W. Wells. (1995) Cooperativity Manifest in the Binding Properties of Purified Cardiac Muscarinic Receptors. Journal of Biological Chemistry 270:38, pages 22488-22499.
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Gary L. Peterson, Arazdordi Toumadje, W. Curtis JohnsonJr.Jr. & Michael I. Schimerlik. (1995) Purification of Recombinant Porcine m2 Muscarinic Acetylcholine Receptor from Chinese Hamster Ovary Cells. Journal of Biological Chemistry 270:30, pages 17808-17814.
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Walter K. Vogel, Valerie A. Mosser, Dylan A. Bulseco & Michael I. Schimerlik. (1995) Porcine m2 Muscarinic Acetylcholine Receptor-Effector Coupling in Chinese Hamster Ovary Cells. Journal of Biological Chemistry 270:26, pages 15485-15493.
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Cynthia R. Ward, Gregory S. Kopf & Bayard T. Storey. (2005) Solubilization and partial purification from mouse sperm membranes of the specific binding activity for 3‐quinuclidinyl benzilate, a potent inhibitor of the zona pellucida‐induced acrosome reaction. Molecular Reproduction and Development 39:4, pages 423-432.
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Ago Rinken, Kimihiko Kameyama, Tatsuya Haga & Lorentz Engström. (1994) Solubilization of muscarinic receptor subtypes from baculovirus infected sf9 insect cells. Biochemical Pharmacology 48:6, pages 1245-1251.
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Graham S. Baldwin, Rosemary Chandler, Boris Grego, Michael R. Ruibira, K.Lin Seet & Janet Weinstock. (1994) Isolation and partial amino acid sequence of A 78 kDa porcine gastrin-binding protein. International Journal of Biochemistry 26:4, pages 529-538.
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Beth Musser, Michael E. Morgan, Mark Leid, Thomas F. Murray, Joel Linden & Robert E. Vestal. (1993) Species comparison of adenosine and β-adrenoceptors in mammalian atrial and ventricular myocardium. European Journal of Pharmacology: Molecular Pharmacology 246:2, pages 105-111.
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Gerhard HILF, Christine KUPPRION, Thomas WIELAND & Karl H. JAKOBS. (2005) Dissociation of guanosine 5′‐[γ‐thio]triphosphate from guanine‐nucleotide‐binding regulatory proteins in native cardiac membranes. European Journal of Biochemistry 204:2, pages 725-731.
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Amy Hauck Newman, Joseph Covington, Marvin Oleshansky, Barrington W. Jackson, Ben Avi Weissman, Haim Leader & Peter K. Chiang. (1990) Aprophit: An irreversible antagonist for muscarinic receptors. Biochemical Pharmacology 40:6, pages 1357-1364.
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Anu KŎiv, Ago Rinken & Jaak Järv. (1990) Fluidity of detergent micelles plays an important role in muscarinic receptor solubilization. Journal of Biosciences 15:3, pages 149-152.
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Gerhard HILF, Peter GIERSCHIK & Karl H. JAKOBS. (2005) Muscarinic acetylcholine receptor‐stimulated binding of guanosine 5′‐ O ‐(3‐thiotriphosphate) to guanine‐nucleotide‐binding proteins in cardiac membranes . European Journal of Biochemistry 186:3, pages 725-731.
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Gerhard Hilf & Karl H. Jakobs. (1989) Activation of cardiac G-proteins by muscarinic acetylcholine receptors: regulation by Mg2+ and Na+ ions. European Journal of Pharmacology: Molecular Pharmacology 172:2, pages 155-163.
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M. SOKOLOVSKY. 1989. 431 509 .
Michael Schimerlik. 1990. The Muscarinic Receptors. The Muscarinic Receptors 93 124 .
Mark Leid, Paul H. Franklin & Thomas F. Murray. (1988) Labeling of A1 adenosine receptors in porcine atria with the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine. European Journal of Pharmacology 147:1, pages 141-144.
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Lawrence C. Rosenbaum, Dean A. Malencik, Sonia R. Anderson, Michael R. Tota & Michael I. Schimerlik. (2002) Phosphorylation of the porcine atrial muscarinic acetylcholine receptor by cyclic AMP-dependent protein kinase. Biochemistry 26:25, pages 8183-8188.
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Michael R. Tota, Kimberly R. Kahler & Michael I. Schimerlik. (2002) Reconstitution of the purified porcine atrial muscarinic acetylcholine receptor with purified porcine atrial inhibitory guanine nucleotide binding protein. Biochemistry 26:25, pages 8175-8182.
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Charles W. Luetje, Craig Brumwell, Marjorie Gainer Norman, Gary L. Peterson, Michael I. Schimerlik & Neil M. Nathanson. (2002) Isolation and characterization of monoclonal antibodies specific for the cardiac muscarinic acetylcholine receptor. Biochemistry 26:22, pages 6892-6896.
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Avi AshkenaziJohn W. WinslowErnest G. PeraltaGary L. PetersonMichael I. SchimerlikDaniel J. CaponJanakiraman Ramachandran. (1987) An M2 Muscarinic Receptor Subtype Coupled to Both Adenylyl Cyclase and Phosphoinositide Turnover. Science 238:4827, pages 672-675.
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Jesse Baumgold, Carl Merril & Elliot S. Gershon. (1987) Loss of pirenzepine regional selectivity following solubilization and partial purification of the putative M1 and M2 muscarinic receptor subtypes. Molecular Brain Research 2:1, pages 7-14.
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Gary L. Peterson, Larry C. Rosenbaum, David J. Broderick & Michael I. Schimerlik. (2002) Physical properties of the purified cardiac muscarinic acetylcholine receptor. Biochemistry 25:11, pages 3189-3202.
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Christine Cremo & Michael I. Schimerlik. (2002) Photoaffinity labeling of the solubilized, partially purified muscarinic acetylcholine receptor from porcine atria by p-azidoatropine methyl iodide. Biochemistry 23:15, pages 3494-3501.
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