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Journal of Liquid Chromatography Volume 15, 1992 - Issue 2
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Original Articles

A Rapid Method For Phospholipid Class Separation by HPLC using an Evaporative Light-Scattering Detector

W. S. Letter VAREX Corporation 4000 Blackburn Lane Burtonsville, Maryland, 20866
Pages 253-266 | Published online: 23 Sep 2006

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Maciej Łukawski, Paulina Dałek, Tomasz Borowik, Aleksander Foryś, Marek Langner, Wojciech Witkiewicz & Magdalena Przybyło. (2020) New oral liposomal vitamin C formulation: properties and bioavailability. Journal of Liposome Research 30:3, pages 227-234.
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Jiann‐Tsyh Lin. (2007) HPLC Separation of Acyl Lipid Classes. Journal of Liquid Chromatography & Related Technologies 30:14, pages 2005-2020.
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Carlie McClintic, David M. Remick, Jeffrey A. Peterson & Donald S. Risley. (2003) Novel Method for the Determination of Piperazine in Pharmaceutical Drug Substances Using Hydrophilic Interaction Chromatography and Evaporative Light Scattering Detection. Journal of Liquid Chromatography & Related Technologies 26:18, pages 3093-3104.
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Andrea L. Guisbert, V. Scott Sharp, Jeffrey A. Peterson & Donald S. Risley. (2000) ENANTIOMERIC SEPARATION OF AN AMPA ANTAGONIST USING A CHIROBIOTIC T™ COLUMN WITH HPLC AND EVAPORATIVE LIGHT-SCATTERING DETECTION. Journal of Liquid Chromatography & Related Technologies 23:7, pages 1019-1028.
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Jeffrey A. Peterson, Leslie J. Lorenz, Donald S. Risley & Beverly J. Sandmann. (1999) AMINO ACID ANALYSIS OF PEPTIDES USING HPLC WITH EVAPORATIVE LIGHT SCATTERING DETECTION. Journal of Liquid Chromatography & Related Technologies 22:7, pages 1009-1025.
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C.-D. Chang & D.J. Harris. (1998) Reversed-Phase High Performance Liquid Chromatographic Analysis of Cationic Lipid-Based Gene Transfer Agents. Journal of Liquid Chromatography & Related Technologies 21:8, pages 1119-1136.
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MichaelD. Lantz, DonaldS. Risley & JeffreyA. Peterson. (1997) Simultaneous Resolution and Detection of a Drug Substance, Impurities, and Counter Ion Using A Mixed-Mode HPLC Column with Evaporative Light Scattering Detection. Journal of Liquid Chromatography & Related Technologies 20:9, pages 1409-1422.
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Witold Miszkiewicz & Jan Szymanowski. (1996) Operational Characteristics of the Evaporative Light Scattering Detector Used in Analysis of Ethoxylated Alcohols. Journal of Liquid Chromatography & Related Technologies 19:7, pages 1013-1032.
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DonaldS. Risley & JeffreyA. Peterson. (1995) A High-Performance Liquid Chromatography Method for the Quantitation of Impurities in an NMDA Antagonist Using Evaporative Light Scattering Detection. Journal of Liquid Chromatography 18:15, pages 3035-3048.
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B.H. Klein & J.W. Dudenhausen. (1994) Simultaneous Determination of Phospholipid Classes and the Major Molecular Species of Lecithin in Human Amniotic Fluid by HPLC. Journal of Liquid Chromatography 17:5, pages 981-998.
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A.I. Hopia & V.-M. Ollilainen. (1993) Comparison of the Evaporative Light Scattering Detector (ELSD) and Refractive Index Detector (RID) in Lipid Analysis. Journal of Liquid Chromatography 16:12, pages 2469-2482.
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Articles from other publishers (56)

Harshika Arora, Mitch D. Culler & Eric A. Decker. (2022) Production of a High-Phosphatidylserine Lecithin That Synergistically Inhibits Lipid Oxidation with α-Tocopherol in Oil-in-Water Emulsions. Foods 11:7, pages 1014.
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Casimir C. AkohFereidoon Shahidi, P. Ambigaipalan & P.K.J.P.D. Wanasundara. 2017. Food Lipids. Food Lipids 131 166 .
Shiro Mawatari, Seira Hazeyama & Takehiko Fujino. (2016) Measurement of Ether Phospholipids in Human Plasma with HPLC-ELSD and LC/ESI-MS After Hydrolysis of Plasma with Phospholipase A 1 . Lipids 51:8, pages 997-1006.
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A.K.M. Asaduzzaman & Byung-Soo Chun. (2015) Quality characteristics of lecithin isolated from deoiled mackerel ( Scomber japonicus ) muscle using different methods. Journal of Industrial and Engineering Chemistry 21, pages 620-626.
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Jun-Ho Yun, Hye-Youn Lee, A.K.M. Asaduzzaman & Byung-Soo Chun. (2013) Micronization and characterization of squid lecithin/polyethylene glycol composite using particles from gas saturated solutions (PGSS) process. Journal of Industrial and Engineering Chemistry 19:2, pages 686-691.
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Seung-Mi Lee, A.K.M. Asaduzzaman & Byung-Soo Chun. (2012) Characterization of Lecithin Isolated from Anchovy (Engraulis japonica) Residues Deoiled by Supercritical Carbon Dioxide and Organic Solvent Extraction. Journal of Food Science 77:7, pages C773-C778.
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Abdelkader Ali-Nehari & Byung-Soo Chun. (2012) Characterization of purified phospholipids from krill (Euphausia superba) residues deoiled by supercritical carbon dioxide. Korean Journal of Chemical Engineering 29:7, pages 918-924.
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William W. Christie. 2012. Advances in Lipid Methodology. Advances in Lipid Methodology 77 107 .
H. Singh, A. Thompson, W. Liu & M. Corredig. 2012. Encapsulation Technologies and Delivery Systems for Food Ingredients and Nutraceuticals. Encapsulation Technologies and Delivery Systems for Food Ingredients and Nutraceuticals 287 318 .
Jun-Ho Yun, Seung-Mi Lee, Jung-Nam Park & Byung-Soo Chun. (2012) Particle Formation of Lecithin Process with Particles from Gas-Saturated Solutions using Supercritical Carbon Dioxide. APCBEE Procedia 2, pages 22-26.
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Jos F. Brouwers. (2011) Liquid chromatographic–mass spectrometric analysis of phospholipids. Chromatography, ionization and quantification. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1811:11, pages 763-775.
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Mónica Narváez-Rivas, Emerenciana Gallardo, José Julián Ríos & Manuel León-Camacho. (2011) A new high-performance liquid chromatographic method with evaporative light scattering detector for the analysis of phospholipids. Application to Iberian pig subcutaneous fat. Journal of Chromatography A 1218:22, pages 3453-3458.
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Md. Salim Uddin, Hideki Kishimura & Byung-Soo Chun. (2011) Isolation and Characterization of Lecithin from Squid (Todarodes pacificus) Viscera Deoiled by Supercritical Carbon Dioxide Extraction. Journal of Food Science 76:2, pages C350-C354.
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P Wanasundara & Fereidoon Shahidi. 2008. Food Lipids. Food Lipids.
Eva Persson, Lars Löfgren, Göran Hansson, Bertil Abrahamsson, Hans Lennernäs & Ralf Nilsson. (2007) Simultaneous assessment of lipid classes and bile acids in human intestinal fluid by solid-phase extraction and HPLC methods. Journal of Lipid Research 48:1, pages 242-251.
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Erlend Hvattum, Steinar Uran, Anne Gunvor Sandbæk, Anders Å. Karlsson & Tore Skotland. (2006) Quantification of phosphatidylserine, phosphatidic acid and free fatty acids in an ultrasound contrast agent by normal-phase high-performance liquid chromatography with evaporative light scattering detection. Journal of Pharmaceutical and Biomedical Analysis 42:4, pages 506-512.
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Lynda Webster, Pam Walsham, Yusuf Ahmed, Sarah Richards, Steve Hay, Mike Heath & Colin F. Moffat. (2006) Development and application of an analytical method for the determination of storage lipids, fatty acids and fatty alcohols in Calanus finmarchicus . Journal of Separation Science 29:9, pages 1205-1216.
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Onno B. Bleijerveld, Martin Houweling, Michael J. Thomas & Zheng Cui. (2006) Metabolipidomics: Profiling metabolism of glycerophospholipid species by stable isotopic precursors and tandem mass spectrometry. Analytical Biochemistry 352:1, pages 1-14.
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Donald S. Risley, Wendy Q. Yang & Jeffrey A. Peterson. (2006) Analysis of mannitol in pharmaceutical formulations using hydrophilic interaction liquid chromatography with evaporative light-scattering detection. Journal of Separation Science 29:2, pages 256-264.
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Brian W. Pack & Donald S. Risley. (2005) Evaluation of a monolithic silica column operated in the hydrophilic interaction chromatography mode with evaporative light scattering detection for the separation and detection of counter-ions. Journal of Chromatography A 1073:1-2, pages 269-275.
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Ivan I. Senin, Doris Höppner-Heitmann, Olga O. Polkovnikova, Valeriya A. Churumova, Natalya K. Tikhomirova, Pavel P. Philippov & Karl-Wilhelm Koch. (2004) Recoverin and Rhodopsin Kinase Activity in Detergent-resistant Membrane Rafts from Rod Outer Segments. Journal of Biological Chemistry 279:47, pages 48647-48653.
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A. Shah, C.C. Akoh, R.T. Toledo & M. Corredig. (2004) Isolation of a phospholipid fraction from inedible egg. The Journal of Supercritical Fluids 30:3, pages 303-313.
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Wei-Nong Zhang, Hai-Bo He, Yu-Qi Feng & Shi-Lu Da. (2004) Separation of phosphatidylcholine and phosphatidylethanolamine by using high-performance displacement chromatography. Journal of Chromatography A 1036:2, pages 145-154.
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Miroslav Kovačevič, Regina Leber, Sepp D. Kohlwein & Walter Goessler. (2004) Application of inductively coupled plasma mass spectrometry to phospholipid analysis. J. Anal. At. Spectrom. 19:1, pages 80-84.
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M. Corredig, R.R. Roesch & D.G. Dalgleish. (2003) Production of a Novel Ingredient from Buttermilk. Journal of Dairy Science 86:9, pages 2744-2750.
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A. M. Descalzo, E. M. Insani & N. A. Pensel. (2003) Light-scattering detection of phospholipids resolved by HPLC. Lipids 38:9, pages 999-1003.
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A Sala Vila, A.I Castellote-Bargalló, M Rodrı́guez-Palmero-Seuma & M.C López-Sabater. (2003) High-performance liquid chromatography with evaporative light-scattering detection for the determination of phospholipid classes in human milk, infant formulas and phospholipid sources of long-chain polyunsaturated fatty acids. Journal of Chromatography A 1008:1, pages 73-80.
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Haruo Shimada, Naoki Nemoto, Yasuo Shida, Tairo Oshima & Akihiko Yamagishi. (2002) Complete Polar Lipid Composition of Thermoplasma acidophilum HO-62 Determined by High-Performance Liquid Chromatography with Evaporative Light-Scattering Detection . Journal of Bacteriology 184:2, pages 556-563.
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Rodolfo R. Brenner, Sixta Ayala & Horacio A. Garda. (2001) Effect of dexamethasone on the fatty acid composition of total liver microsomal lipids and phosphatidylcholine molecular species. Lipids 36:12, pages 1337-1345.
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Frantz S. Deschamps, Pierre Chaminade, Danielle Ferrier & Arlette Baillet. (2001) Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography. Journal of Chromatography A 928:2, pages 127-137.
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A López-López, A.I Castellote-Bargalló & M.C López-Sabater. (2001) Direct determination by high-performance liquid chromatography of sn-2 monopalmitin after enzymatic lipase hydrolysis. Journal of Chromatography B: Biomedical Sciences and Applications 760:1, pages 97-105.
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T Seppänen-Laakso, I Laakso, H Vanhanen, K Kiviranta, T Lehtimäki & R Hiltunen. (2001) Major human plasma lipid classes determined by quantitative high-performance liquid chromatography, their variation and associations with phospholipid fatty acids. Journal of Chromatography B: Biomedical Sciences and Applications 754:2, pages 437-445.
Crossref
Ana Ves-Losada, Sabina M. Maté & Rodolfo R. Brenner. (2001) Incorporation and distribution of saturated and unsaturated fatty acids into nuclear lipids of hepatic cells. Lipids 36:3, pages 273-282.
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P. Soudant, F.-L. E. Chu & Y. Marty. (2000) Lipid class composition of the protozoan Perkinsus marinus, an oyster parasite, and its metabolism of a fluorescent phosphatidylcholine analog. Lipids 35:12, pages 1387-1396.
Crossref
Ana M. Bernasconi, Horacio A. Garda & Rodolfo R. Brenner. (2000) Dietary cholesterol induces changes in molecular species of hepatic microsomal phosphatidylcholine. Lipids 35:12, pages 1335-1344.
Crossref
R.R. Brenner, A.M. Bernasconi & H.A. Garda. (2000) Effect of experimental diabetes on the fatty acid composition, molecular species of phosphatidyl-choline and physical properties of hepatic microsomal membranes. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 63:3, pages 167-176.
Crossref
Jeffrey K Yao, Sherry Leonard & Ravinder D Reddy. (2000) Membrane phospholipid abnormalities in postmortem brains from schizophrenic patients. Schizophrenia Research 42:1, pages 7-17.
Crossref
Xiaohui Yang & John A. Koropchak. (2000) Condensation nucleation light scattering detection with microbore liquid chromatography for lipid analysis. Journal of Microcolumn Separations 12:4, pages 204-210.
Crossref
C. H. A. van de LEST, BIANCA M. van den HOOGEN, P. R. van WEEREN, J. F. H. M. BROUWERS, L. M. G. van GOLDE & A. BARNEVELD. (2010) Changes in bone morphogenic enzymes and lipid composition of equine osteochondrotic subchondral bone. Equine Veterinary Journal 31:S31, pages 31-37.
Crossref
Tatsuya Sugawara & Teruo Miyazawa. (1999) Separation and determination of glycolipids from edible plant sources by high-performance liquid chromatography and evaporative light-scattering detection. Lipids 34:11, pages 1231-1237.
Crossref
Edward P. C. Lai & Ewa Dabek-Zlotorzynska. (1999) Separation of theophylline, caffeine and related drugs by normal-phase capillary electrochromatography. Electrophoresis 20:12, pages 2366-2372.
Crossref
Jos F.H.M. Brouwers, Elisabeth A.A.M. Vernooij, Aloysius G.M. Tielens & Lambert M.G. van Golde. (1999) Rapid separation and identification of phosphatidylethanolamine molecular species. Journal of Lipid Research 40:1, pages 164-169.
Crossref
S.L Abidi. (1998) Separation procedures for phosphatidylserines. Journal of Chromatography B: Biomedical Sciences and Applications 717:1-2, pages 279-293.
Crossref
S Morera Pons, A.I Castellote Bargalló & M.C López Sabater. (1998) Analysis of human milk triacylglycerols by high-performance liquid chromatography with light-scattering detection. Journal of Chromatography A 823:1-2, pages 475-482.
Crossref
G. A. Picchioni, A. E. Watada, W. S. Conway, B. D. Whitaker & C. E. Sams. (1998) Postharvest Calcium Infiltration Delays Membrane Lipid Catabolism in Apple Fruit. Journal of Agricultural and Food Chemistry 46:7, pages 2452-2457.
Crossref
Jos F.H.M. Brouwers, Barend M. Gadella, Lambert M.G. van Golde & Aloysius G.M. Tielens. (1998) Quantitative analysis of phosphatidylcholine molecular species using HPLC and light scattering detection. Journal of Lipid Research 39:2, pages 344-353.
Crossref
Christer Silversand & Carl Haux. (1997) Improved high-performance liquid chromatographic method for the separation and quantification of lipid classes: application to fish lipids. Journal of Chromatography B: Biomedical Sciences and Applications 703:1-2, pages 7-14.
Crossref
Riku Niemi, Hannu Taipale, Marko Ahlmark, Juoko Vepsäläinen & Tomi Järvinen. (1997) Simultaneous determination of clodronate and its partial ester derivatives by ion-pair reversed-phase high-performance liquid chromatography coupled with evaporative light-scattering detection. Journal of Chromatography B: Biomedical Sciences and Applications 701:1, pages 97-102.
Crossref
J.A. Koropchak, C.L. Heenan & L.B. Allen. (1996) Direct comparison of evaporative light-scattering and condensation nucleation light-scattering detection for liquid chromatography. Journal of Chromatography A 736:1-2, pages 11-19.
Crossref
Mitsuhiro Wada, Kenichiro Nakashima, Naotaka Kuroda, Shuzo Akiyama & Kazuhiro Imai. (1996) Sensitive flow-injection method with peroxyoxalate chemiluminescence detection combined with preparative high-performance liquid chromatography for determination of choline-containing phospholipids in human serum. Journal of Chromatography B: Biomedical Sciences and Applications 678:2, pages 129-136.
Crossref
G. A. Picchioni, A. E. Watada & B. D. Whitaker. (1996) Quantitative high-performance liquid chromatography analysis of plant phospholipids and glycolipids using light-scattering detection. Lipids 31:2, pages 217-221.
Crossref
J.J. Myher & A. Kuksis. (1995) General strategies in chromatographic analysis of lipids. Journal of Chromatography B: Biomedical Sciences and Applications 671:1-2, pages 3-33.
Crossref
G.A. Picchioni, A.E. Watada, W.S. Conway, B.D. Whitaker & C.E. Sams. (1995) Phospholipid, galactolipid, and steryl lipid composition of apple fruit cortical tissue following postharvest CaCl2 infiltration. Phytochemistry 39:4, pages 763-769.
Crossref
M. N. Vaghela & A. Kilara. (1995) Quantitative analysis of phospholipids from whey protein concentrates by high-performance liquid chromatography with a narrow-bore column and an evaporative light-scattering detector. Journal of the American Oil Chemists' Society 72:6, pages 729-733.
Crossref
G.A. PICCHIONI, A.E. WATADA, S. ROY, B.D. WHITAKER & W.P. WERGIN. (2006) Membrane Lipid Metabolism, Cell Permeability, and Ultrastructural Changes in Lightly Processed Carrots. Journal of Food Science 59:3, pages 597-601.
Crossref
M. Demirbuker, P. E. Anderson & L. G. Blomberg. (2005) Miniaturized light scattering detector for packed capillary supercritical fluid chromatography. Journal of Microcolumn Separations 5:2, pages 141-147.
Crossref

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