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Biotechnology & Biotechnological Equipment Volume 30, 2016 - Issue 4
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Articles; Medical Biotechnology

Expression and purification of human epidermal growth factor (hEGF) fused with GB1

Xueming ZhengDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Jiangsu University, Zhenjiang, ChinaCorrespondence[email protected]
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,
Xin WuDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Jiangsu University, Zhenjiang, ChinaView further author information
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Xingli FuJiangsu University Health Science Center, Jiangsu University, Zhenjiang, ChinaView further author information
,
Dongping DaiDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Jiangsu University, Zhenjiang, ChinaView further author information
&
Feihu WangDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Jiangsu University, Zhenjiang, ChinaView further author information
Pages 813-818 | Received 11 Sep 2015, Accepted 14 Mar 2016, Published online: 12 Apr 2016

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Kajal Kachhawaha, Santanu Singh, Khyati Joshi, Priyanka Nain & Sumit K. Singh. (2023) Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: insights from structure-function relationship for novel applications. Preparative Biochemistry & Biotechnology 53:7, pages 728-752.
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Sriwidodo Sriwidodo, Iman Permana Maksum, Toto Subroto, Nasrul Wathoni, Anas Subarnas & Abd Kakhar Umar. (2020) Activity and Effectiveness of Recombinant hEGF Excreted by Escherichia coli BL21 on Wound Healing in Induced Diabetic Mice. Journal of Experimental Pharmacology 12, pages 339-348.
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Articles from other publishers (9)

Arthur Schveitzer Ferreira, Amanda Lopacinski, Michel Batista, Priscila Mazzocchi Hiraiwa, Natalia Fernanda Bueno, Beatriz Gomes Guimarães & Nilson I. T. Zanchin. (2022) Functional EGF domain of the human neuregulin 1α produced in Escherichia coli with accurate disulfide bonds. Molecular Biology Reports 49:12, pages 11715-11727.
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Meenakshi Venkatesan, Cameron Semper, Stig Skrivergaard, Rosa Di Leo, Nathalie Mesa, Martin Krøyer Rasmussen, Jette Feveile Young, Margrethe Therkildsen, Peter J. Stogios & Alexei Savchenko. (2022) Recombinant production of growth factors for application in cell culture. iScience 25:10, pages 105054.
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Shi-Jian Song, Hai-Ping Diao, Byeongho Moon, Areum Yun & Inhwan Hwang. (2022) The B1 Domain of Streptococcal Protein G Serves as a Multi-Functional Tag for Recombinant Protein Production in Plants. Frontiers in Plant Science 13.
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Byung Hoon Jo. (2022) An Intrinsically Disordered Peptide Tag that Confers an Unusual Solubility to Aggregation-Prone Proteins. Applied and Environmental Microbiology 88:7.
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Iman Permana Maksum, Yosua Yosua, Ahmad Nabiel, Riyona Desvy Pratiwi, Sriwidodo Sriwidodo & Ukun M.S. Soedjanaatmadja. (2022) Refolding of bioactive human epidermal growth factor from E. coli BL21(DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity. Heliyon 8:4, pages e09306.
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Arthur Schveitzer Ferreira, Amanda Lopacinski, Michel Batista, Priscila Mazzocchi Hiraiwa, Beatriz Gomes Guimarães & Nilson Ivo Tonin Zanchin. (2022) A toolkit for recombinant production of seven human EGF family growth factors in active conformation. Scientific Reports 12:1.
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Anderson A. E. Santo & Gustavo T. Feliciano. (2021) Genetic Algorithms Applied to Thermodynamic Rational Design of Mimetic Antibodies Based on the GB1 Domain of Streptococcal Protein G: An Atomistic Simulation Study. The Journal of Physical Chemistry B 125:29, pages 7985-7996.
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Yakubu OE. (2017) Review Article: Reasons for Underrating the Potential of Human Epidermal Growth Factor in Medical Applications. Journal of Analytical & Pharmaceutical Research 4:2.
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Iman Permana Maksum, Yosua Yosua, Ahmad Nabiel, Riyona Desvy Pratiwi, Sriwidodo Bardi & Ukun M.S. Soedjanaatmadja. (2021) Refolding of Bioactive Human Epidermal Growth Factor from E. Coli Bl21(DE3) Inclusion Bodies & Evaluations on its in Vitro & in Vivo Bioactivity. SSRN Electronic Journal.
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