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Cytotherapy Volume 8, 2006 - Issue 5
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Original

Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM

I Müller University Children's Hospital Tübingen, Tübingen, GermanyCorrespondence[email protected]
, MSc , MD,
S Kordowich University Children's Hospital Tübingen, Tübingen, Germany
,
C Holzwarth University Children's Hospital Tübingen, Tübingen, Germany
,
C Spano Laboratory of Cell Biology and Advanced Cancer Therapy, Department of Oncology and Hematology, University of Modena and Reggio Emilia, Italy
,
G Isensee University Children's Hospital Tübingen, Tübingen, Germany
,
A Staiber University Children's Hospital Tübingen, Tübingen, Germany
,
S Viebahn University Children's Hospital Tübingen, Tübingen, Germany
,
F Gieseke University Children's Hospital Tübingen, Tübingen, Germany
,
H Langer University Clinic for Internal Medicine III, Tübingen, Germany
,
MP Gawaz University Clinic for Internal Medicine III, Tübingen, Germany
,
EM Horwitz St Jude Children's Research Hospital, Divisions of Stem Cell Transplantation and Experimental Hematology, Tennessee, Memphis, USA
,
P Conte Laboratory of Cell Biology and Advanced Cancer Therapy, Department of Oncology and Hematology, University of Modena and Reggio Emilia, Italy
,
R Handgretinger University Children's Hospital Tübingen, Tübingen, Germany
&
M Dominici Laboratory of Cell Biology and Advanced Cancer Therapy, Department of Oncology and Hematology, University of Modena and Reggio Emilia, Italy
 show all
Pages 437-444 | Published online: 07 Jul 2009

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Khatereh Saei Arezoumand, Effat Alizadeh, Younes Pilehvar-Soltanahmadi, Mohammad Esmaeillou & Nosratollah Zarghami. (2017) An overview on different strategies for the stemness maintenance of MSCs. Artificial Cells, Nanomedicine, and Biotechnology 45:7, pages 1255-1271.
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Morten Juhl, Josefine Tratwal, Bjarke Follin, Rebekka H. Søndergaard, Maria Kirchhoff, Annette Ekblond, Jens Kastrup & Mandana Haack-Sørensen. (2016) Comparison of clinical grade human platelet lysates for cultivation of mesenchymal stromal cells from bone marrow and adipose tissue. Scandinavian Journal of Clinical and Laboratory Investigation 76:2, pages 93-104.
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Zakareya Gamie, Robert J MacFarlane, Alicia Tomkinson, Alexandros Moniakis, Gui Tong Tran, Yehya Gamie, Athanasios Mantalaris & Eleftherios Tsiridis. (2014) Skeletal tissue engineering using mesenchymal or embryonic stem cells: clinical and experimental data. Expert Opinion on Biological Therapy 14:11, pages 1611-1639.
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Lan Huang, Paul J. Critser, Brenda R. Grimes & Mervin C. Yoder. (2011) Human umbilical cord blood plasma can replace fetal bovine serum for in vitro expansion of functional human endothelial colony-forming cells. Cytotherapy 13:6, pages 712-721.
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Patrick Horn, Gudrun Bokermann, Dominik Cholewa, Simone Bork, Thomas Walenda, Carmen Koch, Wolf Drescher, Gabriele Hutschenreuther, Martin Zenke, Anthony D. Ho & Wolfgang Wagner. (2010) Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells. Cytotherapy 12:7, pages 888-898.
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Sunghoon Jung, Arindom Sen, Lawrence Rosenberg & Leo A. Behie. (2010) Identification of growth and attachment factors for the serum-free isolation and expansion of human mesenchymal stromal cells. Cytotherapy 12:5, pages 637-657.
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Giulia Grisendi, Cecilia Annerén, Luigi Cafarelli, Rita Sternieri, Elena Veronesi, Gian Luca Cervo, Stefano Luminari, Michela Maur, Antonio Frassoldati, Giovanni Palazzi, Satoru Otsuru, Franco Bambi, Paolo Paolucci, Conte Pierfranco, Edwin Horwitz & Massimo Dominici. (2010) GMP-manufactured density gradient media for optimized mesenchymal stromal/stem cell isolation and expansion. Cytotherapy 12:4, pages 466-477.
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Murat Aktas, Anja Buchheiser, Amelie Houben, Verena Reimann, Teja Radke, Kathrin Jeltsch, Patrick Maier, W. Jens Zeller & Gesine Kogler. (2010) Good manufacturing practice-grade production of unrestricted somatic stem cell from fresh cord blood. Cytotherapy 12:3, pages 338-348.
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Tino Felka, Richard Schäfer, Peter De Zwart & Wilhelm K. Aicher. (2010) Animal serum-free expansion and differentiation of human mesenchymal stromal cells. Cytotherapy 12:2, pages 143-153.
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Patrick C. Baer, Nadine Griesche, Werner Luttmann, Ralf Schubert, Arlette Luttmann & Helmut Geiger. (2010) Human adipose-derived mesenchymal stem cells in vitro: evaluation of an optimal expansion medium preserving stemness. Cytotherapy 12:1, pages 96-106.
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Bettina Lindroos, Shayne Boucher, Lucas Chase, Hannu Kuokkanen, Heini Huhtala, Riina Haataja, Mohan Vemuri, Riitta Suuronen & Susanna Miettinen. (2009) Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro. Cytotherapy 11:7, pages 958-972.
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Karolina Turnovcova, Katerina Ruzickova, Vaclav Vanecek, Eva Sykova & Pavla Jendelova. (2009) Properties and growth of human bone marrow mesenchymal stromal cells cultivated in different media. Cytotherapy 11:7, pages 874-885.
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Minoo Battiwalla & Peiman Hematti. (2009) Mesenchymal stem cells in hematopoietic stem cell transplantation. Cytotherapy 11:5, pages 503-515.
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Chiara Capelli, Agnese Salvadè, Olga Pedrini, Valentina Barbui, Elisa Gotti, Gianmaria Borleri, Benedetta Cabiati, Daniela Belotti, Paolo Perseghin, Piero Bellavita, Andrea Biondi, Ettore Biagi, Alessandro Rambaldi, Josee Golay & Martino Introna. (2009) The washouts of discarded bone marrow collection bags and filters are a very abundant source of hMSCs. Cytotherapy 11:4, pages 403-413.
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Ariane Tormin, Jan C. Brune, Eleonor Olsson, Jeanette Valcich, Ulf Neuman, Tor Olofsson, Sten-Eirik Jacobsen & Stefan Scheding. (2009) Characterization of bone marrow-derived mesenchymal stromal cells (MSC) based on gene expression profiling of functionally defined MSC subsets. Cytotherapy 11:2, pages 114-128.
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R Schäfer, M Dominici, I Müller, E Horwitz, T Asahara, Jwm Bulte, K Bieback, K Le Blanc, Hj Bühring, Mc Capogrossi, F Dazzi, R Gorodetsky, R Henschler, R Handgretinger, J Kajstura, Pj Kluger, C Lange, I von Luettichau, H Mertsching, H Schrezenmeier, Kd Sievert, D Strunk, C Verfaillie & H Northoff. (2009) Basic research and clinical applications of non-hematopoietic stem cells, 4–5 April 2008, Tübingen, Germany. Cytotherapy 11:2, pages 245-255.
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P Horn, S Bork, P Horn, S Bork, A Diehlmann, T Walenda, V Eckstein, Ad Ho & W Wagner. (2008) Isolation of human mesenchymal stromal cells is more efficient by red blood cell lysis. Cytotherapy 10:7, pages 676-685.
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AD Ho, W Wagner & W Franke. (2008) Heterogeneity of mesenchymal stromal cell preparations. Cytotherapy 10:4, pages 320-330.
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D. Spitkovsky & J. Hescheler. (2008) Adult mesenchymal stromal stem cells for therapeutic applications. Minimally Invasive Therapy & Allied Technologies 17:2, pages 79-90.
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M. Haack‐Sorensen, T. Friis, L. Bindslev, S. Mortensen, H. E. Johnsen & J. Kastrup. (2008) Comparison of different culture conditions for human mesenchymal stromal cells for clinical stem cell therapy. Scandinavian Journal of Clinical and Laboratory Investigation 68:3, pages 192-203.
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I Titorencu, Vv Jinga, E Constantinescu, Av Gafencu, C Ciohodaru, I Manolescu, C Zaharia & M Simionescu. (2007) Proliferation, differentiation and characterization of osteoblasts from human BM mesenchymal cells. Cytotherapy 9:7, pages 682-696.
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R Schäfer, M Dominici, I Müller, F Dazzi, K Bieback, K Godthardt, K Le Blanc, R Meisel, R Pochampally, R Richter, T Skutella, G Steinhoff, M Mitterberger, Hp Wendel, J Wiskirchen, R Handgretinger & H Northoff. (2007) Progress in characterization, preparation and clinical applications of non-hematopoietic stem cells, 29–30 September 2006, Tübingen, Germany. Cytotherapy 9:4, pages 397-405.
Read now
A Salvadè, D Belotti, E Donzelli, G D'Amico, G Gaipa, G Renoldi, F Carini, M Baldoni, Em Pogliani, G Tredici, A Biondi & E Biagi. (2007) GMP-grade preparation of biomimetic scaffolds with osteo-differentiated autologous mesenchymal stromal cells for the treatment of alveolar bone resorption in periodontal disease. Cytotherapy 9:5, pages 427-438.
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